165,511 results

1. pD649-HAsp-DLL4-COMP5AP-AviTag-9xHis

   Plasmid

   #157453

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   DLL4 (DLL4 Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-intron-hfCas13d-pA

   Plasmid

   #195864

   Purpose

   To express hfCas13d

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   high fidelity (hf) version of CasRx (hfCas13d)
   

   Use

   AAV

   Mutation

   Yes: GTGGAATACATTACCAACGTGGTGTACGTG

   Promoter

   CMV

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKIIa-hM3D(Gq)-mCherry-WPRE-pA(Short)

   Plasmid

   #183705

   Purpose

   AAV to express hM3D(Gq)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit

   Depositor

   Jonathan Ploski

   Insert

   hM3D(Gq)
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   CaMKIIa

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVTFrep4-creb

   Plasmid

   #182057

   Purpose

   Plasmids for production of LV-based TFactivity reporter

   Depositor

   Kentaro Abe

   Article

   Abe et al iScience. 2022 Feb 14;25(3):103927. doi:

   Insert

   Rep4 and Ref4
   

   Use

   Lentiviral

   Promoter

   synthetic minimal promoter

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. FR_GFP

   Plasmid

   #31302

   Depositor

   Gerhart Ryffel

   Article

   Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

   Insert

   green fluorescent protein
   

   Expression

   Mammalian

   Available Since

   Oct. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEX-tdTomato

   Plasmid

   #28306

   Depositor

   Edward Boyden

   Has Service

   AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   tdTomato
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB_mU6_enh-gRNA_Puro-T2A-BFP

   Plasmid

   #235571

   Purpose

   Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   sgRNA backbone
   

   Use

   CRISPR

   Available Since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmScarlet3-LaminB_C1

   Plasmid

   #189776

   Purpose

   Construct for labelling nuclear envelopes in mammaiian cells using mScarlet3-LaminB fusion

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-LaminB
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS DRR mCherry Donor EF1a BFP

   Plasmid

   #98896

   Purpose

   Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   Neomycin-T2A-mCherry-GFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. eGFP-KIF5B

    Plasmid

    #172203

    Purpose

    Expresses KIF5B tagged with eGFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

    Insert

    KIF5B (Kif5b Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C1-FLAG-Ku80

    Plasmid

    #46958

    Purpose

    Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    Ku80 (XRCC5 Human)
    

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS_4xMma PylT_FLAG-Mma PylRS

    Plasmid

    #140009

    Purpose

    Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZVsE_SC12

    Plasmid

    #219722

    Purpose

    Zika virus stable E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    ZKV sE SC12
    

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A264W, T267R, S286W, S287P, G29K, A25M

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3-U6-sgRNA-PGK-puromycin

    Plasmid

    #51133

    Purpose

    expresses sgRNA from the U6 promoter.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCBL101-RUBY

    Plasmid

    #199723

    Purpose

    Expresses betalain biosynthesis genes under CaMV 35S promoter

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    35S-RUBY
    

    Expression

    Plant

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. iCas

    Plasmid

    #84232

    Purpose

    Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

    Depositor

    Meng How Tan

    Article

    Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

    Insert

    Cas9
    

    Tags

    2A-OFP co-expression and ERT2-ERT2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hIBA1a-GFP-miR124T

    Plasmid

    #214147

    Purpose

    AAV vector to restrict GFP expression in microglia.

    Depositor

    Chun-Li Zhang

    Article

    Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

    Has Service

    AAV5

    Insert

    GFP
    

    Use

    AAV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti- V6.3 Ultra

    Plasmid

    #106172

    Purpose

    lentiviral CMV driven eGFP-P2A-T2A, Blasticidin selectable

    Depositor

    Ewa Snaar-Jagalska

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    T2A, P2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. ER-GCaMP6-210

    Plasmid

    #86919

    Purpose

    Fluorescent reporter for ER calcium signaling

    Depositor

    Timothy Ryan

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    ER-GCaMP6-210
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

    Promoter

    CAMKII

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

    Plasmid

    #62723

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV1 and AAV5

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    human synapsin promoter

    Available Since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV-HA-UTX

    Plasmid

    #24168

    Purpose

    Mammalian expression of human UTX with HA tag

    Depositor

    Kristian Helin

    Article

    Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

    Insert

    UTX (KDM6A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCLBW cox8 EGFP mCherry

    Plasmid

    #78520

    Purpose

    Fluorescent mitophagy reporter

    Depositor

    David Chan

    Article

    Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

    Insert

    none
    

    Use

    Retroviral

    Tags

    cox8 EGFP mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. P021-mPB-ENGRAM-All-in-one-PGK-BFP-P2A-Blast

    Plasmid

    #239972

    Purpose

    Updated ENGRAM plasmid that is ready for both 5' and 3'-FT ENGRAM cloning

    Depositor

    Jay Shendure

    Article

    Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-ClpX

    Plasmid

    #234733

    Purpose

    Human-codon optimized E. coli ClpX in mammalian cells

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    ClpX
    

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lck-mScarlet-I

    Plasmid

    #98821

    Purpose

    In vivo visualization of the plasma membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lck
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHR-iCasp9

    Plasmid

    #199441

    Purpose

    Express inducible Caspase9 in mammalian cells

    Depositor

    Chao Tang

    Article

    Yuan et al Cell Death Dis. 2022 Jan 10;13(1):34. do

    Insert

    inducible Caspase9 (CASP9 Human)
    

    Use

    Lentiviral

    Tags

    DmrB and mCherry

    Expression

    Bacterial and Mammalian

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDEST53-LRRK2-G2019S

    Plasmid

    #25045

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Glycine 2019 mutated to Serine

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 7xSMAD_RE::FLuc

    Plasmid

    #124531

    Purpose

    SMAD2 FLuc luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    SMAD FLuc reporter
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pLX317-EGFP

    Plasmid

    #115439

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MGAT2-RpHLuorin2

    Plasmid

    #171718

    Purpose

    Cis-/medial-Golgi expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)
    

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. dCas13d-dsRBD-APEX2

    Plasmid

    #154939

    Purpose

    TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. MSCV Puro

    Plasmid

    #68469

    Purpose

    Retroviral plasmid with multiple cloning site and puromycin resistance

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Puromycin Resistance
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    pGK

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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33. HA GSK3 beta wt pcDNA3

    Plasmid

    #14753

    Purpose

    Mammalian expression of human GSK3 beta with HA tag

    Depositor

    Jim Woodgett

    Article

    He et al Nature. 1995 Apr 13. 374(6523):617-22.

    Insert

    GSK3 beta (GSK3B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pLminP_Luc2P_RE27

    Plasmid

    #90369

    Purpose

    Type II Interferon - STAT1/STAT1 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    STAT1

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJT260 Lenti pEF-dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast

    Plasmid

    #187336

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA-dCas9-HA

    Plasmid

    #61355

    Purpose

    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SpyTag003-mKate2

    Plasmid

    #133452

    Purpose

    Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-mKate2
    

    Tags

    GSGSGS linker and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pExp-NusA

    Plasmid

    #112571

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8His and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTol2-10xUAS-Voltron

    Plasmid

    #119039

    Purpose

    UAS-driven expresion of Voltron in zebrafish

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Use

    Zebrafish expression

    Promoter

    E1b

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFLAG-YAP1

    Plasmid

    #66853

    Purpose

    Express FLAG-tagged YAP1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    YAP1 (YAP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge