161,202 results

1. TetO-FUW-Klf4

   Plasmid

   #20322

   Depositor

   Rudolf Jaenisch

   Article

   Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

   Insert

   Klf4 (Klf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas3cRh

   Plasmid

   #133773

   Purpose

   Expresses all necessary components of Type I-C CRISPR-Cas system

   Depositor

   Joseph Bondy-Denomy

   Article

   Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

   Inserts

   RhaR (rhaR )
   RhaS (rhaS )
   pRhaBAD
   I-C crRNA
   Cas3
   Cas5
   Cas8
   Cas7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV.Syn.GCaMP6f.WPRE.SV40

   Plasmid

   #100837

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

   Promoter

   Synapsin

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-SIRT6

   Plasmid

   #102325

   Purpose

   Human SIRT6 for mammalian expression

   Depositor

   Hening Lin

   Article

   Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

   Insert

   SIRT6 (SIRT6 Human)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFastBac-CHD7

   Plasmid

   #170141

   Purpose

   Expresses CHD7 in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRIS-PITChv2-dTAG-BSD (BRD4)

   Plasmid

   #91795

   Purpose

   PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   FKBP_F36V-2xHA-P2A-BSD
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promotorless

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUGW

   Plasmid

   #14883

   Purpose

   3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresion

   Depositor

   David Baltimore

   Article

   Lois et al Science. 2002 Feb 1. 295(5556):868-72.

   Insert

   flap-Ub promoter-GFP-WRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-hPOMC1-26-sbGLuc-P2A-dTomato

   Plasmid

   #176704

   Purpose

   neural activity dependent release of sbGLuc from presynaptic vesicles

   Depositor

   Ute Hochgeschwender

   Article

   Prakash et al Commun Biol. 2022 Jan 11;5(1):33. doi: 1

   Inserts

   Gaussia luciferase, slow burn
   dTomato
   

   Use

   AAV

   Tags

   P2A and hPOMC1-26

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. KARS_pNIC-Bio3

   Plasmid

   #153051

   Purpose

   Expression construct for producing Avi tagged Lysyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   KARS (KARS1 Human)
   

   Use

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-Gfi1

    Plasmid

    #158531

    Purpose

    Expresses human Gfi1 in mammalian cells

    Depositor

    Neil Segil

    Article

    Menendez et al Elife. 2020 Jun 30;9. pii: 55249. doi: 1

    Insert

    Growth Factor Independent Protein 1 (GFI1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBabe-Hygro-GFP

    Plasmid

    #61215

    Purpose

    Retrovirus expressing GFP

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUltra

    Plasmid

    #24129

    Purpose

    3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interest

    Depositor

    Malcolm Moore

    Article

    Lou et al PLoS One. 2012;7(3):e33093. doi: 10.1371

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pREDusk-MCS

    Plasmid

    #188970

    Purpose

    For red light-repressed bacterial expression of gene of interest

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115209

    Purpose

    Expresses PbuCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PbuCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+) NAPstar3b

    Plasmid

    #232736

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar3b in mammalian cells.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar3b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Tet-O-FUW-Myt1l

    Plasmid

    #27152

    Depositor

    Marius Wernig

    Article

    Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

    Insert

    Myt1l (Myt1l Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-hPhyBY276H-mCherry-HRasCT

    Plasmid

    #100540

    Purpose

    Expresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome B (1-908 aa, Y276H mutation) (PHYB Mustard Weed)
    

    Use

    Tags

    mCherry fused with HRas C-terminus for plasma mem…

    Expression

    Mammalian

    Mutation

    Human codon optimized PhyB (1-908 aa), Y276H muta…

    Promoter

    CAG promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-Wnt6

    Plasmid

    #35913

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt6 (WNT6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent Mutation G (567) --> A

    Promoter

    CMV

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiGuide Blast

    Plasmid

    #199622

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFC333

    Plasmid

    #87844

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    ble (bleomycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-nef-CIAO2-TVA-mCherry

    Plasmid

    #149297

    Purpose

    Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing.

    Depositor

    Edward Callaway

    Article

    Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

    Insert

    TVA:mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Start codon removed.

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDH-SIRT6-H133Y

    Plasmid

    #102327

    Purpose

    Human SIRT6 H133Y for mammalian expression

    Depositor

    Hening Lin

    Article

    Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

    Insert

    SIRT6 (SIRT6 Human)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    changed histidine 133 to tyrosine

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLH_Scr15

    Plasmid

    #100537

    Purpose

    CEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.

    Depositor

    Bernd Müller-Röber

    Article

    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

    Inserts

    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    CreC, CreN, and SV40 NLS

    Expression

    Yeast

    Mutation

    N-terminal version of PhyB (AA 1-621)/ Mutation o…

    Promoter

    FBA1 and TDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115216

    Purpose

    Expresses Pin2Cas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    Pin2Cas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKM19

    Plasmid

    #123655

    Purpose

    Encodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cells

    Depositor

    Kevin Maringer

    Article

    Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pIEx

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Cdc2-HA

    Plasmid

    #1888

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdc2 (CDK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Cytoplasmic cpGFP

    Plasmid

    #215709

    Purpose

    Expresses cpGFP in the cytoplasm

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    cpGFP
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and cytoplasmic localization sequence

    Expression

    Mammalian

    Mutation

    circularly permuted GFP

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115208

    Purpose

    Expresses PinCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PinCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pArk313

    Plasmid

    #200257

    Purpose

    Ark313 rep/cap

    Depositor

    Justin Eyquem

    Article

    Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

    Insert

    Ark313 Rep/Cap
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBabe-puro-RIPK3

    Plasmid

    #78829

    Purpose

    retroviral expression of RIPK3

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

31. pDONR223-PRPS1

    Plasmid

    #23588

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PRPS1 (PRPS1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

32. NIR-AG

    Plasmid

    #210728

    Purpose

    NIR light-activated GSDMD

    Depositor

    Vladislav Verkhusha

    Article

    Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

    Insert

    NIR-AG (GSDMD Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115207

    Purpose

    Expresses BzoCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    BzoCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNIC28-BSA4-G-L11

    Plasmid

    #127830

    Purpose

    Production of recombinant human ribosomal protein L11

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPL11 (RPL11 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. NbAlfa-GFP2

    Plasmid

    #201485

    Purpose

    BRET2 detection of ALFA-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbAlfa-GFP2
    

    Use

    Tags

    GFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBabe-puro-mRipk3

    Plasmid

    #78830

    Purpose

    retroviral expression of mRIPK3

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

37. pCrepusculo

    Plasmid

    #190156

    Purpose

    Regulates bacterial expression of target gene in blue-light-repressed manner. Based on Nakamurella multipartita PAL.

    Depositor

    Andreas Moeglich

    Article

    Ranzani et al ACS Synth Biol. 2022 Oct 21;11(10):3482-

    Type

    Empty backbone

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pENTR221-FOS

    Plasmid

    #79511

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human FOS CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    FOS (FOS Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pWUR 1+2 tetO mut89

    Plasmid

    #80102

    Purpose

    Encodes a set of wild-type Cas1+2 and a mutant set of Cas1+2

    Depositor

    George Church

    Article

    Shipman et al Science. 2016 Jun 9. pii: aaf1175.

    Inserts

    Cas1 (cas1 )
    Cas2 (cas2 )
    Cas1 (cas1 )
    Cas2 (cas2 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q24H, P202Q, G241D, E276D, L297Q

    Promoter

    Available since

    Dec. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCR3.1-mutLuc

    Plasmid

    #106458

    Purpose

    luciferase reporter, CMV promoter (3 NFkB sites mutated)

    Depositor

    Georg Weber

    Article

    He et al Biochem Biophys Res Commun. 2004 Aug 13;

    Insert

    luciferase reporter, CMV promoter (3 NFkB sites mutated)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    3 NFkB sites mutated

    Promoter

    Available since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge