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pAAV-pMecp2-dSaCas9-VP160-pU6-sgRNA

Plasmid

#158987

Purpose

Vector D encodes pAAV-pMecp2-dSaCas9-VP160-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP160

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-sgGAL4-1

Plasmid

#46915

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgGAL4-1
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV and U6

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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KIF20A D5.2 gRNA

Plasmid

#90718

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIF20A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

KIF20A (Guide Designation D5.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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G1397 DddAtox-C–SaKKH-Cas9(D10A)

Plasmid

#157840

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

G1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Use

CRISPR

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_KDM1A_1

Plasmid

#86297

Purpose

Encodes gRNA for 3' target of human KDM1A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM1A (KDM1A Human)

Use

CRISPR

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KDM1A_2

Plasmid

#86298

Purpose

Encodes gRNA for 3' target of human KDM1A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM1A (KDM1A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBBK84 pHomL-RPL18Bp-PDR16-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179068

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Scarlet(rfp), a marker for the lipid droplet

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PDR16-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK79 pHomL-RPL18Bp-Scarlet(rfp)-Link-YPT7-SSA1t-HomR (AmpR)

Plasmid

#179063

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-YPT7, a marker for the vacuole, late endosome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-YPT7

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-u(S)Cas9

Plasmid

#127383

Purpose

Expresses Cas9 at high level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(S)-Cas9

Use

CRISPR

Expression

Insect

Promoter

UAST

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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FEN1 C10.3 gRNA

Plasmid

#90689

Purpose

3rd generation lentiviral gRNA plasmid targeting human FEN1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FEN1 (Guide Designation C10.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_eA3A-nCas9

Plasmid

#163535

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHelper-T7IS1

Plasmid

#140631

Purpose

Expresses ShCAST under the T7 promoter and an empty sgRNA

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64T-hLbCpf1

Plasmid

#107187

Purpose

plasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

hLbCpf1

Use

CRISPR

Tags

NLS-3xHA

Promoter

sp6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pT3TS-zAsCpf1

Plasmid

#107185

Purpose

plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

AsCpf1

Use

CRISPR

Tags

NLS-3xHA

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA p53, mitfa:Cas9

Plasmid

#118841

Purpose

Targets tp53 and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

tp53 gRNA (tp53 Zebrafish)

Use

CRISPR; Tol2

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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Sa-dCas9-NLS-3xFLAG/pcDNA3.1

Plasmid

#98041

Purpose

Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

Sa-dCas9-NLS-3xFLAG

Use

CRISPR

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

D10A + H557A

Promoter

CMV

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB3052(gRNA X-4, XI-3, XII-5)

Plasmid

#73294

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PB_EF1a-CasRx-msfGFP-2A-Blast

Plasmid

#226010

Purpose

CasRx protein for RNA targeting in piggyBac backbone

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Insert

CasRx RNA-targeting nuclease

Use

CRISPR

Tags

msfGFP

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBBK80 pHomL-RPL18Bp-Scarlet(rfp)-Link-VAM3-SSA1t-HomR (AmpR)

Plasmid

#179064

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VAM3, a marker for the vacuole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-VAM3

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-CBE1

Plasmid

#195367

Purpose

dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAG

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

hA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI

Use

CRISPR; Gateway compatible ha3a/y130f-xten plus 3…

Tags

3xflag

Expression

Plant

Promoter

ZmUBI1

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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SMC6 G4.4 gRNA

Plasmid

#90899

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC6 (Guide Designation G4.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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SMC6 G3.4 gRNA

Plasmid

#90898

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC6 (Guide Designation G3.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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BRCA1 A9.2 gRNA

Plasmid

#90548

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA1 (Guide Designation A9.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_AHR_2

Plasmid

#101077

Purpose

Encodes gRNA for 3' target of human AHR

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against AHR (AHR Human)

Use

CRISPR

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAH-CTX1-rhadCas9-native

Plasmid

#129392

Purpose

Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

S. pyogenes dCas9

Use

CRISPR

Expression

Bacterial

Mutation

Aspartate 10 to Alanine, Histidine 840 to Alanine

Promoter

PrhaBAD

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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FEN1 C9.3 gRNA

Plasmid

#90688

Purpose

3rd generation lentiviral gRNA plasmid targeting human FEN1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FEN1 (Guide Designation C9.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK90 pHomL-TEF2p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)

Plasmid

#179074

Purpose

Vector containing a yeast integration cassette for expressing TEF2p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TEF2p-Scarlet(rfp)-Link-2xPH

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CHEK2 C3.2 gRNA

Plasmid

#90628

Purpose

3rd generation lentiviral gRNA plasmid targeting human CHEK2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CHEK2 (Guide Designation C3.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR348 FANCF

Plasmid

#47510

Purpose

human gRNA expression vector targeting FANCF

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-FANCF (FANCF Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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Rad51AP1 B12.3 gRNA

Plasmid

#90869

Purpose

3rd generation lentiviral gRNA plasmid targeting human Rad51AP1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

Rad51AP1 (Guide Designation B12.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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AA027

Plasmid

#216007

Purpose

Fragmid fragment: (Cas protein) nearly PAM-less

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9-SpRY_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSL0374 (His-MBP-Cas8_Cas7_Cas6)

Plasmid

#130639

Purpose

Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCas8, VchCas7, VchCas6

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on Cas8

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pC012 LwCas13a locus with nontargeting spacer

Plasmid

#91904

Purpose

LwCas13a locus into pACYC184 with nontargeting spacer

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LwCas13a with non-targeting spacer

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 16, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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MSP2253

Plasmid

#70704

Purpose

Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Mutation

E782K, N968K and R1015H in SaCas9

Promoter

T7

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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OmEF1aCas9P2ApuroSB

Plasmid

#165484

Purpose

Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells

Depositor

Dietmar Kueltz

Article

Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

Insert

Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)

Use

CRISPR; Transposon

Promoter

Oreochromis mossambicus EF1 alpha promoter (OmEF1…

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC5-dual-dCas9VP48-sgTetO

Plasmid

#48237

Purpose

Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9VP48 and sgTetO

Use

CRISPR

Tags

HA-tag and VP48

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCas3-Sm

Plasmid

#208001

Purpose

Expresses all necessary components of Type I-C CRISPR-Cas system

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Inserts

RhaR (rhaR )
RhaS (rhaS )
pRhaBAD
I-C crRNA
Cas3
Cas5
Cas8

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_Anc689-nCas9

Plasmid

#163526

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

Anc689-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSDest2-NmeCas9-NLS-3XHA-NLS

Plasmid

#87448

Purpose

Mammalian expression of wild type Nme Cas9

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Nme Cas9

Use

CRISPR

Tags

3XHA, SV40 NLS, and cMyc-like NLS

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
LIN52 D6.4 gRNA

Plasmid

#90735

Purpose

3rd generation lentiviral gRNA plasmid targeting human LIN52

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

LIN52 (Guide Designation D6.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 24,790 results for: SPR