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Showing: 4651 - 4700 of 33749 results

  4. pSUMO-β′-COPI-WD40

    Plasmid
    #180753
    Purpose
    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1
    Insert
    β′-COPI-WD40 (SEC27 Budding Yeast)
    Tags
    N-terminal strep tag, His-tag, and SUMO tag
    Expression
    Bacterial
    Mutation
    Encodes residues 1-301; deleted 302-889; mutated …
    Promoter
    T7
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    180753-plasmid-map-sequence-id-356139

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  9. pTYB11-PTBP1-RRM2CCtoSS

    Plasmid
    #89154
    Purpose
    recombinant expression of PTBP1-RRM2 C250S,C251S in e.coli
    Depositor
    Frédéric Allain
    Article
    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n
    Insert
    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
    Tags
    Intein and chitin binding domain
    Expression
    Bacterial
    Mutation
    mutations: Cys 250 to Ser, Cys 251 to Ser
    Promoter
    T7
    Available Since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89154-plasmid-map-sequence-id-175723

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  15. CIBN-rTetR

    Plasmid
    #183919
    Purpose
    Optogenetic CIBN localizer domain coupled to reverse Tet repressor; binds tetO sites upon doxycycline addition; CIBN is bound by PHR upon blue light exposure
    Depositor
    Karsten Rippe
    Article
    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22
    Insert
    CIBN-rTetR (CIB1 Synthetic, Mustard Weed)
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    June 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183919-plasmid-map-sequence-id-364132

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  16. Flag-M4-AR-W741C

    Plasmid
    #171247
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor mutant that binds to bicalutamide: AR-Trp741Cys (AR-W741C)
    Depositor
    Steven Balk
    Article
    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.
    Insert
    3xFlag-tagged human androgen receptor Trp741Cys mutant (AR Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Trp741Cys (AR-W741C)
    Promoter
    CMV
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171247-plasmid-map-sequence-id-336010

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  18. MLM3720

    Plasmid
    #49944
    Purpose
    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a control
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x FLAG Tet1CDmut RH-3 (RHOXF2 Human)
    Use
    TALEN
    Tags
    3x FLAG
    Mutation
    catalytic domain of TET1 inactive (H1671Y and D16…
    Promoter
    EF1a
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  24. pLKO.1-shGBP1.1.mKO2

    Plasmid
    #85209
    Purpose
    TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.
    Depositor
    Sandra Martha Gomes Dias
    Article
    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1
    Insert
    GBP1 (guanylate binding protein 1) (GBP1 Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    RNA polymerase III promoter for human U6 snRNA fo…
    Available Since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85209-plasmid-map-sequence-id-169780

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  25. JA740

    Plasmid
    #49939
    Purpose
    Expresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in EGFP for use as a control
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD GFP (TET1 Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  29. MLM3762

    Plasmid
    #49947
    Purpose
    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a control
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x FLAG Tet1CDmut RH-4 (RHOXF2 Human)
    Use
    TALEN
    Tags
    3x FLAG
    Mutation
    catalytic domain of TET1 inactive (H1671Y and D16…
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  33. JA524

    Plasmid
    #49938
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in EGFP for use as a control
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD GFP (TET1 Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  35. pTXB1-hMeCP2-R270X

    Plasmid
    #48092
    Purpose
    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-R270X
    Depositor
    Huda Zoghbi
    Article
    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10
    Insert
    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    Expression
    Bacterial
    Mutation
    Mxe intein/chitin binding domain tagged, expresse…
    Available Since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  38. pTXB1-hMeCP2-G273X

    Plasmid
    #48093
    Purpose
    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-G273X
    Depositor
    Huda Zoghbi
    Article
    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10
    Insert
    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    Expression
    Bacterial
    Mutation
    Mxe intein/chitin binding domain tagged, expresse…
    Available Since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  41. pDGB3_Omega1_Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos (GB1677)

    Plasmid
    #160641
    Purpose
    Module for estradiol-inducible exp of the PhiC31 integrase gene. Includes TU for constitutive exp of an estradiol-inducible transcription activator and TU for estradiol-inducible exp of PhiC31.
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    ERLexABDGal4AD / PhiC31
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos
    Available Since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160641-plasmid-map-sequence-id-321669

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  47. pCW-CKS2-HA

    Plasmid
    #247439
    Purpose
    HA-tagged mouse CKS2 cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.
    Depositor
    Richard Treisman
    Article
    Nielsen et al J Cell Sci. 2025 Nov 7:jcs.264444. doi:
    Inserts
    CKS2 (Cks2 Mouse)
    IRES2:EGFP
    Use
    Lentiviral; Dox-inducible
    Tags
    HA
    Expression
    Mammalian
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247439-plasmid-map-sequence-id-491536

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  48. pCW-Myoferlin-HA

    Plasmid
    #247440
    Purpose
    HA-tagged human Myoferlin cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.
    Depositor
    Richard Treisman
    Article
    Nielsen et al J Cell Sci. 2025 Nov 7:jcs.264444. doi:
    Inserts
    Myoferlin (MYOF Human)
    IRES2:EGFP
    Use
    Lentiviral; Dox-inducible
    Tags
    HA
    Expression
    Mammalian
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247440-plasmid-map-sequence-id-490943

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  50. pCMV-scFv-GCN4-StayGold-IRESv4-HaloTag-tdMCP-tdP2A-tdPCP-mCherry

    Plasmid
    #241143
    Purpose
    Expresses Anti-GCN4 scFv-StayGold, HaloTag-tdMCP, and tdPCP-mCherry to track mature and nascent SunTag-tagged proteins and track MS2 and PP7-tagged mRNA
    Depositor
    Tim Stasevich
    Article
    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi
    Inserts
    Anti-GCN4 scFv
    tdMCP
    tdPCP
    Tags
    (n2)oxStayGold(c4) E147D, 3x mCherry, and HaloTag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    241143-plasmid-map-sequence-id-487014

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Showing: 4651 - 4700 of 33749 results