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12,913 results

Showing: 4701 - 4750 of 12913 results

  1. pHybr_r2a>mG2b-srt-his

    Plasmid
    #124804
    Purpose
    HDR template to knock-in mouse IgG2b Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2b producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2b
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124804-plasmid-map-sequence-id-244314

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  9. tdTomato-MCHR1-N-10

    Plasmid
    #58114
    Purpose
    Localization: Cilium, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    MCHR1
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pET-3a_mb2m

    Plasmid
    #211368
    Purpose
    Expresses murine b2m
    Depositor
    Adnane Achour
    Insert
    b2m (B2m Mouse)
    Expression
    Bacterial
    Available Since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    211368-plasmid-map-sequence-id-425189

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  13. pB80-KIF1A(1-365)-VVD52C-mCherry-SSPB(micro)

    Plasmid
    #174633
    Purpose
    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVD52C-mCherry-SSPB(micro) (Kif1a Mouse, Synthetic)
    Tags
    mCherry-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174633-plasmid-map-sequence-id-346989

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  15. pHybr_r2a>mG2a-srt-his

    Plasmid
    #124803
    Purpose
    HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2a
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124803-plasmid-map-sequence-id-244318

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  17. mEmerald-NPHP1-N-18

    Plasmid
    #54204
    Purpose
    Localization: Cilium, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    NPHP1
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. AAV-KrasWT/sgKras/Cre

    Plasmid
    #99848
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99848-plasmid-map-sequence-id-193786

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  26. pBS-mSema3C

    Plasmid
    #16426
    Depositor
    Jonathan Raper
    Insert
    pBS-Sema3C (Sema3c Mouse)
    Use
    In situ template
    Mutation
    None
    Available Since
    Dec. 14, 2007
    Availability
    Academic Institutions and Nonprofits only

  30. Cntn2-Fc-His

    Plasmid
    #72066
    Purpose
    Expresses the extracellular region of the Contactin 2 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Cntn2 (Cntn2 Mouse)
    Tags
    Fc-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72066-plasmid-map-sequence-id-137383

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  35. RSRF-Luc-2mut

    Plasmid
    #31819
    Depositor
    Astar Winoto
    Article
    Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.
    Insert
    Mutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    two CTATATTTAG RSRF sites mutated to CGATATTTCG
    Promoter
    mutated MEF2-dependent (-307 to -242 of Nur77/NR4…
    Available Since
    Aug. 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  37. mEmerald-H1-10

    Plasmid
    #54109
    Purpose
    Localization: Nucleus/Histones, Excitation: 488, Emission: 509
    Depositor
    Michael Davidson
    Insert
    H1 (H1f0 Mouse)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. mCherry-Alpha1-Catenin-N-18

    Plasmid
    #54977
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Alpha1-Catenin
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  42. Ntng1.a-AP-His

    Plasmid
    #71984
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform a protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.a (Ntng1 Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    71984-plasmid-map-sequence-id-131170

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  45. pAAV-DYRK3-2A-mCherry

    Plasmid
    #118287
    Purpose
    Expresses mouse DYRK3 tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (Dyrk3 Mouse)
    Use
    AAV
    Tags
    2A peptide and mCherry
    Expression
    Mammalian
    Promoter
    CMV with beta global intron
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118287-plasmid-map-sequence-id-229469

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Showing: 4701 - 4750 of 12913 results