159,352 results

1. Flag-TRIM24

   Plasmid

   #28138

   Depositor

   Michelle Barton

   Article

   Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

   Insert

   TRIM24 (TRIM24 Human)
   

   Use

   Tags

   FLAGX2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-CMV-PEmax-IRES-ZeoR

   Plasmid

   #207353

   Purpose

   Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   Prime editor max (PEmax) enzyme
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LentiV_Cas9_puro

   Plasmid

   #108100

   Purpose

   Lentiviral expression plasmid of spCas9 with puromycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS promoter

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCH49 (crRNA expression vector)

   Plasmid

   #217338

   Purpose

   CROP-seq crRNA expression vector with GFP and neo marker

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   AsDR-filler-AsDR8, GFP-neo
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG415GPD-ccdB

   Plasmid

   #14146

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. RFP-ATF4

   Plasmid

   #125237

   Purpose

   ATF4 expression vector

   Depositor

   Rozita Yarmand

   Article

   Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

   Insert

   ATF4 (ATF4 Human)
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. HDAC6 Flag

   Plasmid

   #13823

   Purpose

   Mammalian expression of human histone deacetylase 6 with flag tag

   Depositor

   Eric Verdin

   Article

   Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

   Insert

   HDAC6 (HDAC6 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

   Plasmid

   #83841

   Purpose

   Fluorescent probe for G1/S transition and M/G1 transition

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRES-EGFP-puro

   Plasmid

   #45567

   Depositor

   Michael McVoy

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   IRES-EGFP-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   Phcmv (strong human cytomegalovirus immediate ear…

   Available since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0756

    Plasmid

    #141685

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    JUND (JUND Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. gCH128 (non-targeting crRNA)

    Plasmid

    #217339

    Purpose

    Non-targeting crRNA

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    crNT-3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRDA_887

    Plasmid

    #201164

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3.3 N-MYC TIP60

    Plasmid

    #213003

    Purpose

    expresses human TIP60

    Depositor

    Sheng-cai Lin

    Article

    Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

    Insert

    KAT5 (KAT5 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AiP5003 pAAV-Syn-DIO-TVA66T-dTom-CVS-N2cG

    Plasmid

    #176285

    Purpose

    Cre-dependent AAV virus

    Depositor

    Shenqin Yao, The Allen Institute for Brain Science

    Article

    Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

    Insert

    TVA66T-dTomato-rabies Glycoprotein
    

    Use

    Tags

    dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCyCBE

    Plasmid

    #226505

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in cyanobacteria

    Depositor

    Weizhong Chen

    Article

    Li et al Commun Biol. 2024 Aug 14;7(1):994. doi:

    Inserts

    APOBEC1-Cas9D10A
    sgRNA
    sacB gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti6/V5-p53_R175H

    Plasmid

    #22936

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R175H Mutant

    Promoter

    Available since

    March 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-HcRed

    Plasmid

    #11152

    Purpose

    Mammalian expression vector for expression of HcRed

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Insert

    HcRed1 from Heteractis crispa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR_PGK_LaG16_2_synNotch_Gal4VP64

    Plasmid

    #85421

    Purpose

    Lentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic Notch

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    LaG16_2 GFP nanobody synNotch Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW57.1 CMYC

    Plasmid

    #164145

    Purpose

    Doxycycline inducible expression of c-MYC

    Depositor

    Richard Possemato

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Doxycycline inducible promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPR

    Plasmid

    #42875

    Purpose

    A crRNA expression plasmid for targeting a specific sequence.

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    CRISPR-BsaI
    

    Use

    CRISPR; E.coli

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-lyso-pHluorin

    Plasmid

    #70113

    Purpose

    Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    lyso-pHluorin (Cd63 Aequorea victoria, Mouse)
    

    Use

    Tags

    HA-Tag and pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHCMV_DENV1_PrME

    Plasmid

    #207263

    Purpose

    Mammalian expression of Dengue virus 1 PrME protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    DENV1_PrME (POLY Dengue virus 1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-hSyn-ASAP3-WPRE

    Plasmid

    #132331

    Purpose

    expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAGD335

    Plasmid

    #219499

    Purpose

    pAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoter

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Eco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-MbPylRS(DiZPK)

    Plasmid

    #91706

    Purpose

    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

    Depositor

    P. Chen

    Article

    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

    Inserts

    pylS
    pylT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

    Promoter

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ML1Nwt_in_pEGFP-C1

    Plasmid

    #67797

    Purpose

    Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain wildtype version) fused to EGFP

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    ML1N*2-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLPT107

    Plasmid

    #85525

    Purpose

    Repressilator with triple reporter

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Inserts

    mCfp
    mVenus
    tetR
    lacI
    cI
    mKate2
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-PGK-Hygro-PIP-FUCCI

    Plasmid

    #138714

    Purpose

    Lentiviral expression of the cell cycle marker PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Lentiviral

    Tags

    mCherry and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Factor IX donor

    Plasmid

    #182141

    Purpose

    Factor IX donor for twinPE and Bxb1 mediated insertion at ALB

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MIG-Luciferase-IRES-GFP

    Plasmid

    #75021

    Purpose

    Encodes the expression of Luciferase and GFP

    Depositor

    Saradhi Mallampati

    Insert

    Luciferase
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEGFP-C1-AR Q48

    Plasmid

    #86428

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Poly-glutamine expansion, G130D

    Promoter

    CMV

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TFORF0793

    Plasmid

    #142692

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ATF5 (ATF5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHCMV_FLUAV_HA

    Plasmid

    #207273

    Purpose

    Mammalian expression of Influenza A virus HA protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    FLUAV_HA (HA Influenza A virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1 PRDM16

    Plasmid

    #15503

    Depositor

    Bruce Spiegelman

    Article

    Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPYL-ABI

    Plasmid

    #233650

    Purpose

    Cellular colocalization assay (PYL/ABI) for the CIP OP (opabactin) or the CIP ABA (abscisic acid)

    Depositor

    Richard Wombacher

    Article

    Poschko et al ACS Chem Biol. 2025 Jan 27. doi: 10.1021

    Insert

    TOMM20-mCherry-PYL-P2A-eGFP-ABI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBR.DT-A

    Plasmid

    #35955

    Depositor

    Jacqueline Lees

    Article

    Danielian et al Oncogene. 2008 Nov 20;27(51):6561-70. Ep

    Insert

    diphtheria toxin A
    

    Use

    Bac recombineering

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRSETB/mStayGold(c4)

    Plasmid

    #212018

    Purpose

    For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    mStayGold(c4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. piggyBAC-H2B mCherry-XL (R125K)

    Plasmid

    #189926

    Purpose

    Expresses nuclear-localized mCherry-XL (R125K) in mammalian cells

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

    Insert

    mCherry-XL (R125K)
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    differs from mCherry by R125K, W143S, I161V, Q163…

    Promoter

    CMV7

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCDNA3.1(+)-NEMOc

    Plasmid

    #189933

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pT18mobsacB

    Plasmid

    #72648

    Purpose

    sucrose counter-selection allelic exchange vector for Pseudomonas syringae

    Depositor

    Brian Kvitko

    Type

    Empty backbone

    Use

    Sucrose counter-selection allelic exchange vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 18, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge