161,799 results

1. Lck-Halo_pTwistCMVBetaGlobWPRENeo

   Plasmid

   #221900

   Purpose

   Expresses Halo7 targeted to the plasma membrane

   Depositor

   Janelia Integrative Imaging

   Insert

   Lck-Halo7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. RoL10X_pET29b

   Plasmid

   #215427

   Purpose

   Expression construct for RoL10X gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   RoL10X
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V209L/D262G/E190C/E238C/F173Y/V175F/G177C/Q197H/F…

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. ThcCut1_pET29b

   Plasmid

   #215429

   Purpose

   Expression construct for ThcCut1 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   ThcCut1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GPR182-DuET

   Plasmid

   #213272

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR182 (GPR182 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPLICSFAST-ER-PM-P2A

   Plasmid

   #214893

   Purpose

   Expresses equimolar Plasma Membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PM Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-ER-PM-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. px552-U6-gRNA1-U6-gRNA2-CMV-eGFP

   Plasmid

   #211760

   Purpose

   Paired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

   Depositor

   Glenn Yiu

   Article

   Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

   Insert

   gRNA1 and gRNA2 targeting VEGF-A (VEGFA M. mulatta (rhesus macaque), Mouse, Human)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF0751

   Plasmid

   #143729

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HIVEP1 (HIVEP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-AM

   Plasmid

   #206513

   Purpose

   AAV packaging vector containing AAV2 rep and AM cap and partial AAV2 p5 promoter

   Depositor

   Mark Kay

   Article

   Gonzalez-Sandoval et al Nat Commun. 2023 Apr 28;14(1):2448. doi:

   Insert

   AM capsid
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   p5

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-Ruby2-NS

   Plasmid

   #166569

   Purpose

   Multisite Gateway middle entry clone encoding mRuby2. Parton lab clone DJA

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   Ruby2
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. acta1-mRuby2-VHH[GFP]

    Plasmid

    #163856

    Purpose

    Zebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRX

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    VHH[GFP]
    

    Use

    In vitro transcription of rna

    Tags

    Ruby2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT3TS-Cavin4b-TurboID

    Plasmid

    #163851

    Purpose

    In vitro transcription of Cavin4b-TurboID. Parton lab clone JTA

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    Cavin4b (cavin4b Zebrafish)
    

    Use

    In vitro transcription of rna

    Tags

    TurboID

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT3TS-CD44b-TurboID

    Plasmid

    #163850

    Purpose

    In vitro transcription of CD44b-TurboID. Parton lab clone JTJ

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    CD44b (cd44b Zebrafish)
    

    Use

    In vitro transcription of rna

    Tags

    TurboID

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT3TS-BASU-EGFP

    Plasmid

    #163845

    Purpose

    In vitro transcription of BASU-EGFP. Parton lab clone JRR

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    BASU
    

    Use

    In vitro transcription of rna

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2plus-TDP-43 M337V

    Plasmid

    #162609

    Purpose

    Allows for transcription of mutant TDP-43-M337V mRNA for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M337V

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGMC00005

    Plasmid

    #166722

    Purpose

    sgRNA against human ASNS

    Depositor

    Raj Chari

    Article

    Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

    Insert

    ASNS (ASNS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGMC00006

    Plasmid

    #166723

    Purpose

    sgRNA against human ASNS

    Depositor

    Raj Chari

    Article

    Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

    Insert

    ASNS (ASNS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJOE4847.2

    Plasmid

    #135086

    Purpose

    rhamnose-inducible expression vector

    Depositor

    Josef Altenbuchner

    Article

    Motejadded et al Biotechnol Lett. 2009 Apr;31(4):543-9. d

    Insert

    MalE-6xHis-Ulp1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLC30A7

    Plasmid

    #132148

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC30A7 (SLC30A7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pLENTI_RPS10_K139R

    Plasmid

    #127137

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS10 (RPS10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K139R

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLENTI_RPS10_K138R

    Plasmid

    #127136

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS10 (RPS10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K138R

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pmU6-gRNA

    Plasmid

    #53187

    Purpose

    Expresses the S. pyogenes sgRNA from the mouse U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pEGFP-2xFYVE

    Plasmid

    #140047

    Purpose

    Expresses GFP-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. Lifeact-mCherry

    Plasmid

    #193300

    Purpose

    mammalian transient expression - expression of Lifeact-mCherry

    Depositor

    Klaus Hahn

    Article

    Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

    Insert

    Lifeact
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV4 p65

    Plasmid

    #21966

    Depositor

    Warner Greene

    Article

    Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

    Insert

    NFkB p65 subunit (RELA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    This construct was the product of 3 subcloning pr…

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pIVT-R2Tg-WT

    Plasmid

    #238105

    Purpose

    For IVT of wildtype R2Tg editor, codon optimized for mammalian expression.

    Depositor

    Feng Zhang

    Article

    Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

    Insert

    Wildtype R2Tg, mammalian codon optimized
    

    Use

    For ivt

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Hsp90 HA

    Plasmid

    #22487

    Depositor

    William Sessa

    Article

    Garcia-Cardena et al Nature. 1998 Apr 23. 392(6678):821-4.

    Insert

    Hsp90 HA (HSP90AB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJRH-1180 U6-reci Gag-pol v2

    Plasmid

    #201915

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Gag-pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. lenti_dCas9-KRAB-MeCP2

    Plasmid

    #122205

    Purpose

    Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Lentiviral

    Tags

    KRAB-MeCP2

    Expression

    Mammalian

    Mutation

    Cas9 (D10A, H840A)

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PPRE X3-TK-luc

    Plasmid

    #1015

    Purpose

    PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

    Depositor

    Bruce Spiegelman

    Article

    Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

    Insert

    PPRE
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV enAsCas12f-YHAM-sgRNA_v7

    Plasmid

    #208356

    Purpose

    Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-YHAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F48Y/S188H/V232A/E316M

    Promoter

    CMV

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMRX-No-HaloTag7-mGFP-LC3

    Plasmid

    #184901

    Purpose

    Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Use

    Tags

    HaloTag7 and monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pML104-KanMx4

    Plasmid

    #83476

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    KanMX4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLSL.R.Ly

    Plasmid

    #227697

    Purpose

    Reengineered geminiviral replicon vector

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNK497

    Plasmid

    #219754

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Paxillin-pEGFP

    Plasmid

    #15233

    Depositor

    Rick Horwitz

    Article

    Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

    Insert

    paxillin (PXN Chicken)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGP-AAV-syn-jGCaMP8m-WPRE

    Plasmid

    #162375

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #190033

    Purpose

    vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized hfCas13X
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y672A,Y676A

    Promoter

    CBh, SV40, hU6

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LentiV_Cas9_puro

    Plasmid

    #108100

    Purpose

    Lentiviral expression plasmid of spCas9 with puromycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pT3EF1aH-myr-Akt

    Plasmid

    #179909

    Purpose

    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

    Depositor

    Xin Chen

    Article

    Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMevT

    Plasmid

    #17815

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge