We narrowed to 8,601 results for: Ott
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Plasmid#86750PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertAtg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 1-200PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Venus-MDGA2
Plasmid#82544PurposeOverexpression vector for Venus-MDGA2 in neuronsDepositorInsertMDGA2 (Mdga2 Rat)
UseTagsVenus fluorescent proteinExpressionMammalianMutationPromoterchicken beta actinAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBF_HsPepT2
Plasmid#73944PurposeXenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tagDepositorInsertPepT2 (SLC15A2 Human)
UseMake rna for xenopus oocyte injectionTagsFlagExpressionMutationPromoterbeta globinAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)
Plasmid#99229PurposeMammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.DepositorInsertpr-mEos2
UseTagsLifeactExpressionMammalianMutationA69T for primed conversion applicationsPromoterCMVAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-CDC42
Plasmid#109531PurposeMuscle specific zebrafish expression of mKate2 fused to CDC42DepositorInsertCDC42 (cdc42 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMS1943
Plasmid#82565PurposeHigh copy number plasmid containing Ple266 (S100B MiniPromoter) insert.DepositorInsertPle266
UsePleiades promoter project [sic, pleaides plieades]TagsExpressionMutationPromoterAvailable sinceSept. 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1997
Plasmid#49127PurposeAAV plasmid with SLC6A4 (Ple198) promoter driving expression of iCre.DepositorInsertssAAV-Ple198-iCre
UseAAVTagsExpressionMammalianMutationPromoterSLC6A4Available sinceMay 7, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
actc1b-mKate2-CDC42-DN
Plasmid#109532PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative CDC42DepositorInsertCDC42 (cdc42 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationDNPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
scADGFP
Plasmid#114434PurposeEpilepsy promoter enhanced with Arc elements driving GFP with SV40E polyADepositorInsertGreen fluorescent protein
UseAAVTagsER export sequence FCYENEVExpressionMammalianMutationPromoterNovel synthetic activity-dependent promoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEMS2143
Plasmid#104356PurposeAAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter. Contains WPRE.DepositorInsertssAAV-smCBA-EmGFP-WPRE
UseAAVTagsExpressionMutationPromotersmCBAAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD4 (OTU, aa 22-196)
Plasmid#61413PurposeExpresses human OTUD4 (OTU domain) in E. coli.DepositorInsertOTUD4 (OTU domain-containing protein 4) (OTUD4 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-21 and aa 197-1114.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTarget-RFP-GFP operon
Plasmid#192279PurposeRFP GFP fusion protein expressed under a constitutive promoterDepositorInsertRFP GFP
UseTagsExpressionBacterialMutationPromoterPlacIqAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9 PE2*-3HA
Plasmid#169854PurposeMammalian Expression, SpyCas9 PE2* prime editor with 3HA-tagDepositorInsertSpCas9-H840A-star-M-MLV-3HA
UseTags3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…ExpressionMammalianMutationPromotercmvAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A mKate2-hu_ncOGT D554N
Plasmid#154283PurposeEntry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2ExpressionMutationD554N mutation (Ser/Thr Glycosylation Impaired)PromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-Cezanne2 (OTU+UBA, aa 1-462)
Plasmid#61582PurposeExpresses human Cezanne2 (OTU+UBA domains) in E. coli.DepositorInsertCezanne2 (OTUD7A Human)
UseTagsHis6-GST-3CExpressionBacterialMutationIsoform 2. Deleted aa 463-933.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEMS2031
Plasmid#49134PurposeAAV plasmid with OLIG1 (Ple305) promoter driving expression of iCre.DepositorInsertssAAV-Ple305-iCre
UseAAVTagsExpressionMammalianMutationPromoterOLIG1Available sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA
Plasmid#47455PurposeLITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.DepositorInsertNLS(alpha-imp)-CRY2PHR-NLS-VP64
UseAAVTags2A_GFPExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1YFP8
Plasmid#41669DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
UseTagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; 8 C-terminal-m…PromoterCMVAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-DysF
Plasmid#109492PurposeMuscle specific zebrafish expression of mKate2 fused to dysferlinDepositorInsertDysF (dysf Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Actin-C-18
Plasmid#53978PurposeLocalization: Actin, Excitation: 487, Emission: 509DepositorInsertActin (ACTB Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE
Plasmid#102986PurposeAAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombinationDepositorInsertsmembrane-targeted SNAP protein after FLP-mediated recombination
Nuclear-targeted eGFP after FLP-mediated recombination
UseAAV; Adeno associated viral vectorTagsExpressionMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-std
Plasmid#109423PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB-TAG-HES7
Plasmid#130935PurposePiggyBac vector for DOX-inducible human HES7-IRES-EGFP expression in mammalian cellsDepositorInsertHES7 (HES7 Human)
UseTagsExpressionMammalianMutationPromoterTetOAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mKikGR1 (mKikGR1-V69T)
Plasmid#99227PurposeBacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).DepositorInsertpr-mKikGR1
UseTagsExpressionBacterialMutationmonomeric variant with mutation V69T (Eos notatio…PromoterT7Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC6A2
Plasmid#131891PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A2 (SLC6A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLCKO_PSMD1_sgRNA_5
Plasmid#74181Purposelentiviral vector expressing sgRNA targeting PSMD1DepositorInsertPSMD1 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEThT-RvCAHS12
Plasmid#192468PurposeBacterial expression of RvCAHS12DepositorInsertCAHS12
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1
Plasmid#121831PurposepMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/LSD1 (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
UbG76V-eGFP-V5His_15aa
Plasmid#23969DepositorInsertUb-G76V-eGFP-V5HIS_15aa (UBB Human)
UseTagseGFPExpressionMammalianMutationG76V mutation in Ubiquitin. Ub fused to N-termin…PromoterAvailable sinceApril 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
p6348 MSCV-CMV-Flag-HA-Brd4 1047-1362
Plasmid#31354DepositorInsertBrd4 1047-1362 (BRD4 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HtrA2-EGFP S306A
Plasmid#14122DepositorInsertHtrA2 (HTRA2 Human)
UseTagsEGFPExpressionMammalianMutationS306APromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
(p)odr-3::GFP::EGL-4
Plasmid#100897PurposeGFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.DepositorInsert56-2741: odr-3 promoter
UseTagsExpressionWormMutationPromoterodr-3Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40
Plasmid#163698PurposeAAV vector expressing CaMPARI2_F391W primarily in excitatory neurons in rodent cortexDepositorInsertCaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40
UseAAVTagsFLAG-HA-myc and NES_hisExpressionMammalianMutationPromoterCaMKII fragmentAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
p6347 MSCV-CMV-Flag-HA-Brd4-444-722
Plasmid#31353DepositorInsertBrd4 444-722 (BRD4 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1977
Plasmid#49109PurposeAAV plasmid with C8ORF46 (Ple251) promoter driving expression of iCre.DepositorInsertssAAV-Ple251-icre
UseAAVTagsExpressionMammalianMutationPromoterC8ORF46Available sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
myc-Atg14 S29D
Plasmid#86757PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationS29DPromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSRW1JZ
Plasmid#129528PurposeC. elegans codon-optimized FlincG3 (GFP-based cGMP sensor) expressed in C. elegans ASE neuron pairDepositorInsertFlincG3
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP1
Plasmid#129504PurposeExpresses AvicFP1 constitutively in E. coli (most strains)DepositorInsertAvicFP1
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCKO_PSMD1_sgRNA_1
Plasmid#74180Purposelentiviral vector expressing sgRNA targeting PSMD1DepositorInsertPSMD1 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS1725
Plasmid#105870PurposeHigh copy number plasmid containing iCreERT2-FFrt-KanR-FFrt insertDepositorInsertiCreERT2-FFrt-KanR-FFrt
UseTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-CDC42-DN
Plasmid#109584PurposeMultisite gateway vector for 3' tagging with dominant negative CDC42DepositorInsertCDC42 (cdc42 Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMS1996
Plasmid#49126PurposeAAV plasmid with UGT8 (Ple267) promoter driving expression of iCre.DepositorInsertssAAV-Ple267-iCre
UseAAVTagsExpressionMammalianMutationPromoterUGT8Available sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNCST-AausFP2
Plasmid#129500PurposeExpresses AausFP2 constitutively in E. coli (most strains)DepositorInsertAausFP2
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 S29A
Plasmid#86756PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationS29APromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausGFP
Plasmid#129503PurposeExpresses AausGFP constitutively in E. coli (most strains)DepositorInsertAausGFP
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP2
Plasmid#129505PurposeExpresses AvicFP2 constitutively in E. coli (most strains)DepositorInsertAvicFP2
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p6349 MSCV-CMV-Flag-HA-Brd4 1224-1362
Plasmid#31355DepositorInsertBrd4 1224-1362 (BRD4 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS2157
Plasmid#70118PurposePromoterless AAV plasmid with MCS to insert promoter for expression of EmGFP. Contains WPRE.DepositorInsertssAAV-MCS-EmGFP-WPRE
UseAAVTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L
Plasmid#121833PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/Dnmt3a-3L (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Cln6
Plasmid#78109Purposemammalian expression of mouse Cln6 with C terminal EGFP fusionDepositorInsertCln6 (Cln6 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only