163,819 results

1. pcDNA3-Lyn-AKAR4

   Plasmid

   #61620

   Purpose

   Lipid raft-targeted FRET biosensor for monitoring PKA activity

   Depositor

   Jin Zhang

   Article

   Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

   Insert

   Lyn-AKAR4
   

   Use

   Tags

   Cerulean, N-terminal targeting sequence from Lyn …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

   Plasmid

   #234952

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. cytoplasmic EKAR (Cerulean-Venus)

   Plasmid

   #18679

   Depositor

   Karel Svoboda

   Article

   Harvey et al PNAS 2008 Dec 9;105(49):19264-9

   Insert

   ERK Activity Reporter
   

   Use

   Tags

   monomeric Cerulean and monomeric Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-GRAB_DA1m (AAV9)

   Viral Prep

   #113049-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_DA1m (#113049). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_DA1m plasmid DNA. Synapsin-driven expression of GRAB-DA1m dopamine sensor in neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Yulong Li

   Article

   Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

   Promoter

   Syn

   Tags

   None

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

5. pCMV/SB11

   Plasmid

   #26552

   Depositor

   Perry Hackett

   Article

   Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

   Insert

   Sleeping Beauty
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Methionine 243 to Glutamine Threonine 136 to Arg…

   Promoter

   Available since

   Oct. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221_SLC22A5

   Plasmid

   #132048

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC22A5 (SLC22A5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pENN.AAV.CamKII 0.4.Cre.SV40 (AAV5)

   Viral Prep

   #105558-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4.Cre.SV40 plasmid DNA. CamKII-driven Cre expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   CaMKII

   Tags

   None

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pLCS105

   Plasmid

   #209358

   Purpose

   Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal Dendra2 fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMax_mOrange

   Plasmid

   #177827

   Purpose

   Host Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mOrange
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUFlip-floxed2A-mRFP-; gcry1:BFP -1

    Plasmid

    #173887

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; gcry1: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CaMKIIa-mCherry (AAV9)

    Viral Prep

    #114469-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    CaMKII

    Tags

    mCherry

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

12. Watermelon

    Pooled Library

    #155257

    Purpose

    Lineage tracing library

    Depositor

    Joan Brugge, Aviv Regev

    Article

    Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

    Expression

    Mammalian

    Use

    Lentiviral

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

13. TFORF0481

    Plasmid

    #142638

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HIF1A (HIF1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF3518

    Plasmid

    #144994

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NHLH2 (NHLH2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40

    Plasmid

    #105540

    Purpose

    AAV expression of EGFP-Cre from hSyn promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP-Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHTC-HaloTag_C9orf72:1-481

    Plasmid

    #210987

    Purpose

    Mammalian expression of full-length human C9orf72. C-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    C9orf72:M1-F481 (C9orf72 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60229

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. TCARZ-CBh-GFP-SV40PA-GF3-R4A22

    Plasmid

    #203767

    Purpose

    For production of template RNA for use in PRINT, GFP under CBh promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for GFP
    

    Use

    Ivt template

    Tags

    Expression

    Mutation

    Promoter

    CBh

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTol2-10xUAS-Voltron

    Plasmid

    #119039

    Purpose

    UAS-driven expresion of Voltron in zebrafish

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    E1b

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSMPUW_6.17_Ex_Ef1a-v5-EGFP-SV40-Zeo

    Plasmid

    #128453

    Purpose

    lentiviral expression vector with Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and ZeoResistance gene

    Depositor

    Lindy Barrett

    Insert

    Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Accessory plasmid 1

    Plasmid

    #128569

    Purpose

    for mixing with SPECS library to produce lentivirus

    Depositor

    Timothy Lu

    Article

    Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

    Insert

    ECFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMT525

    Plasmid

    #131392

    Purpose

    L1RP retrotransposition reporter

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    L1RP GFP-AI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Hamdoun lab FP fusion plasmids

    Plasmid Kit

    #1000000033

    Purpose

    Screen fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed, e.g. zebrafish, sea urchin, frog (Xenopus), or worm (C. elegans)

    Depositor

    Amro Hamdoun

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

24. pDONR221_SLC9A2

    Plasmid

    #132222

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC9A2 (SLC9A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pTol1-UAS:ChrimsonR-tdTomato

    Plasmid

    #124231

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    ChrimsonR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMRS-LacI-GFP

    Plasmid

    #220198

    Purpose

    mini-Tn7 GFP expression vector - LacI/pLac promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    LacI/pLac

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PRS_8-eGFP

    Plasmid

    #192589

    Purpose

    AAV-vector for fluorescent labeling of noradrenergic neurons

    Depositor

    Simon Wiegert

    Article

    Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRSX8

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV-shRNA-ctrl

    Plasmid

    #85741

    Purpose

    shRNA ctrl

    Depositor

    Hongjun Song

    Article

    Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

    Insert

    shRNA ctrl
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGGF_RT_FAST_RFP

    Plasmid

    #136979

    Purpose

    Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

    Depositor

    Klaus Theres

    Article

    Rossmann et al Plant J. 2019 Oct 30. doi: 10.1111/tpj.1

    Insert

    seed coat marker RFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

    Promoter

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

    Plasmid

    #125235

    Purpose

    Expresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDONR223_CYP3A5_WT

    Plasmid

    #81823

    Purpose

    Gateway Donor vector containing CYP3A5 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CYP3A5 (CYP3A5 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAG-hHDAC6

    Plasmid

    #21099

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    Histone Deacetylase 6 (HDAC6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

33. pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

    Plasmid

    #117484

    Purpose

    Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    SpyCatcher002-6aa-sfCherry2(1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MCS-3_Endofin_LgBiT

    Plasmid

    #182574

    Purpose

    This plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.

    Depositor

    Bradley McConnell

    Article

    Reyes-Alcaraz et al Commun Biol. 2022 Mar 8;5(1):212. doi: 1

    Insert

    zinc finger FYVE-type (ZFYVE16 Human)
    

    Use

    Tags

    LgBiT (Large BiT)

    Expression

    Mammalian

    Mutation

    FYVE domain Q739-K806

    Promoter

    Herpes simplex virus (HSV)

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-ExRai-AMPKAR

    Plasmid

    #192446

    Purpose

    Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PPDK

    Plasmid

    #64037

    Purpose

    Expression of Pyruvate phosphate dikinase (PPDK) in E. coli

    Depositor

    Vern Schramm

    Article

    Sturm et al Anal Chem. 2009 Apr 15;81(8):2847-53. do

    Insert

    pyruvate phosphate dikinase
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pCAG-Synapto-pHluorin

    Plasmid

    #168510

    Purpose

    Expression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    Synaptophysin-pHluorin (Syp Mouse)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-CAG-mRhubarb713

    Plasmid

    #197207

    Purpose

    Expresses the protein of mRhubarb713 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    mRhubarb713
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTUM4

    Plasmid

    #230905

    Purpose

    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

    Depositor

    Arne Skerra

    Article

    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

    Inserts

    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-ER-AKAR4

    Plasmid

    #64733

    Purpose

    Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    AKAR4
    

    Use

    Tags

    Cerulean, N-terminal 27 amino acids from CYP450, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge