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We narrowed to 22,327 results for: cas9 genes

Showing: 4801 - 4840 of 22327 results

  2. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid
    #115694
    Purpose
    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer
    Article
    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:
    Inserts
    NmeCas9
    Nme-sgRNA
    Use
    CRISPR
    Tags
    NLS and NLS, HA
    Expression
    Insect and Mammalian
    Mutation
    None and fusion of repeat/tracrRNA to make a sgRNA
    Promoter
    EF-1 alpha and U6
    Available since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    115694-plasmid-map-sequence-id-221986

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  5. A3Bi-ctd E255A -Cas9n-UGI-NLS

    Plasmid
    #109429
    Purpose
    Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109429-plasmid-map-sequence-id-212934

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  8. pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V

    Plasmid
    #187959
    Purpose
    Expressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Inserts
    Blasticidin resistance
    FKBP12_F36V
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187959-plasmid-map-sequence-id-373708

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  10. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid
    #217808
    Purpose
    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217808-plasmid-map-sequence-id-432916

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  12. pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid
    #84261
    Purpose
    Expresses AND gate ABA/GA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    GAI-tagBFP-Sp dCas9
    PYL1-VPR-p2a-GID1-ABI
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and PGK
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    84261-plasmid-map-sequence-id-160892

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  13. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

    Plasmid
    #84243
    Purpose
    Expresses ABA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    ABI-tagBFP-Sa dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    84243-plasmid-map-sequence-id-160884

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  14. pJT055 AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #187324
    Purpose
    Donor to install a dual surface marker - fluorescence reporter into the AAVS1 safe harbor using HDR
    Depositor
    Michael Bassik, Lacra Bintu
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF1a
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    187324-plasmid-map-sequence-id-369800

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  15. pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid
    #84257
    Purpose
    Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    GAI-tagBFP-Sp dCas9-ABI
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and PGK
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    84257-plasmid-map-sequence-id-160891

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  16. pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid
    #106895
    Purpose
    Expresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulation
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    GAI-tagBFP-Sp dCas9-ABI
    GID1-KRAB
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and PGK
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    106895-plasmid-map-sequence-id-201653

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  17. Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)

    Plasmid
    #217797
    Purpose
    Variant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Cdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Cdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217797-plasmid-map-sequence-id-432864

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  18. pJT340 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast

    Plasmid
    #187344
    Purpose
    dCas9 effector fusion for manipulating transcription
    Depositor
    Michael Bassik, Lacra Bintu
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast
    Use
    CRISPR and Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    KRAB WSR7EEE
    Promoter
    pEF1a
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    187344-plasmid-map-sequence-id-373294

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  19. pJT242 Lenti pEF-dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast

    Plasmid
    #187327
    Purpose
    dCas9 effector fusion for manipulating transcription
    Depositor
    Michael Bassik, Lacra Bintu
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast
    Use
    CRISPR and Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    KRAB WSR7EEE
    Promoter
    pEF1a
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    187327-plasmid-map-sequence-id-373284

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  20. pJT341 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

    Plasmid
    #195540
    Purpose
    dCas9 effector fusion for manipulating transcription
    Depositor
    Michael Bassik, Lacra Bintu
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast
    Use
    CRISPR and Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    pEF1a
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    195540-plasmid-map-sequence-id-387166

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  22. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  23. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

    Plasmid
    #64217
    Purpose
    Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A
    Depositor
    Ralf Kuehn
    Article
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Inserts
    Cas9
    hU6 promoter; BbsI sites for sgRNA
    H1 promoter; BamHI site for shRNA
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CBh, H1, and U6
    Available since
    Sept. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    64217-plasmid-map-sequence-id-123057

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  24. T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB

    Plasmid
    #110547
    Purpose
    CRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.
    Depositor
    Ichiro Matsumura
    Insert
    dCas9
    Use
    Tags
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pBAD
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    110547-plasmid-map-sequence-id-210556

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  25. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid
    #102245
    Purpose
    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.
    Depositor
    Jacob Corn
    Insert
    dCas9-VPR-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    NLS-VPR-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102245-plasmid-map-sequence-id-354473

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  33. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

    Plasmid
    #62373
    Purpose
    Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells
    Depositor
    David Liu
    Article
    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do
    Insert
    (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
    Use
    Tags
    (-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…
    Expression
    Bacterial
    Mutation
    Y67S amino acid substitution in (-30)GFP renders …
    Promoter
    T7
    Available since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    62373-plasmid-map-sequence-id-263816

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Showing: 4801 - 4840 of 22327 results