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pAdEasy-1

Plasmid

#16400

Purpose

Adenoviral vector for use in AdEasy System.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

Plasmid

#71236

Purpose

Express sgRNA and dCas9-KRAB from lentiviral vector

Depositor

Charles Gersbach

Publication

Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

Inserts

humanized dCas9-KRAB T2A Puro
sgRNA

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

140 Articles

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pTargetF

Plasmid

#62226

Purpose

Constitutive expression of sgRNA without donor editing template DNA

Depositor

Sheng Yang

Publication

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Insert

sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pij23119

Available since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

72 Articles

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SBE4-Luc

Plasmid

#16495

Purpose

Luciferase reporter containing four copies of the Smad binding site

Depositor

Bert Vogelstein

Publication

Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

Insert

Smad binding element

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

82 Articles

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lentiSAMv2

Plasmid

#75112

Purpose

lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Publication

Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EF1A

Available since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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pET16B.Pfu

Plasmid

#12509

Purpose

Bacterial expression of Pfu DNA polymerase

Depositor

Sung-Hou Kim

Publication

Goldman et al Acta Crystallogr D Biol Crystallogr. 199

Insert

Pfu DNA polymerase

Expression

Bacterial

Mutation

Y218H, Q462R, F533L, K559I and V683A (mutations w…

Available since

Sept. 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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FUW-OSKM

Plasmid

#20328

Purpose

Lentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generation

Depositor

Rudolf Jaenisch

Publication

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Myc, Sox2, Pou5f1, Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pAdTrack-CMV

Plasmid

#16405

Purpose

Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

49 Articles

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pHAGE TRE dCas9-KRAB

Plasmid

#50917

Purpose

Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Publication

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and KRAB

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive)

Promoter

TRE

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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lentiCRISPRv2 blast

Plasmid

#98293

Purpose

Variant of lentiCRISPRv2 that confers blasticidin S resistance

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

65 Articles

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pMXs-hKLF4

Plasmid

#17219

Purpose

Retroviral expression of human KLF4

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

KLF4 (KLF4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

120 Articles

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pmScarlet_C1

Plasmid

#85042

Purpose

Mammalian expression of mScarlet

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

mScarlet

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

72 Articles

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pMXs-hSOX2

Plasmid

#17218

Purpose

Retroviral expression of human SOX2

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

SOX2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

113 Articles

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CoV2-Spike-D614G

Plasmid

#177960

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

Depositor

Jennifer Doudna

Publication

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )

Expression

Mammalian

Mutation

D614G

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pmRFP-LC3

Plasmid

#21075

Purpose

Mammalian expression of rat LC3 fused to mRFP

Depositor

Tamotsu Yoshimori

Publication

Kimura et al Autophagy. 2007;3(5):452-60.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

mRFP

Expression

Mammalian

Mutation

First ATG is not included.

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

95 Articles

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HDAC3 Flag

Plasmid

#13819

Purpose

Mammalian expression of human histone deacetylase 3 with flag tag

Depositor

Eric Verdin

Publication

Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

Insert

HDAC3 (HDAC3 Human)

Tags

flag

Expression

Mammalian

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pXR003: CasRx gRNA cloning backbone

Plasmid

#109053

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

Depositor

Patrick Hsu

Publication

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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lentiCRISPRv2 hygro

Plasmid

#98291

Purpose

Variant of lentiCRISPRv2 that confers hygromycin resistance

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

84 Articles

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PB-TRE-dCas9-VPR

Plasmid

#63800

Purpose

SP-dCas9-VPR with doxycycline-inducible expression

Depositor

George Church

Publication

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

dCas9-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

TRE

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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phU6-gRNA

Plasmid

#53188

Purpose

Expresses the S. pyogenes sgRNA from the human U6 promoter

Depositor

Charles Gersbach

Publication

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

47 Articles

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