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169,450 results

Showing: 6701 - 6750 of 169450 results

  1. pQ-Elo-HB-C-A(1-772)

    Plasmid
    #171085
    Purpose
    Co-expresses of WT-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26 amino acids for expression purp…
    Promoter
    T5 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171085-plasmid-map-sequence-id-335501

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  5. pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid
    #50468
    Purpose
    Gs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Insert
    rM3D-IRES-mCitrine
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50468-plasmid-map-sequence-id-75484

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  13. mCherry-ZO1-C-14

    Plasmid
    #55165
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ZO1 (TJP1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55165-plasmid-map-sequence-id-194785

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  14. hprtSAGFP

    Plasmid
    #41594
    Purpose
    In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences
    Depositor
    Maria Jasin
    Article
    Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.
    Insert
    SA-GFP reporter
    Use
    Mouse Targeting; Dsb repair reporter substrate
    Expression
    Mammalian
    Available Since
    Dec. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    41594-plasmid-map-sequence-id-228244

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  15. pSBbi-GP

    Plasmid
    #60511
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60511-plasmid-map-sequence-id-222915

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  24. lentiCRISPR v2-dCas9

    Plasmid
    #112233
    Purpose
    Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2
    Depositor
    Thomas Gilmore
    Article
    Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do
    Inserts
    dCas9
    Porumycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Changed: Aspartic acid 10 to Glycine, Histidine 8…
    Promoter
    EFS-NS
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112233-plasmid-map-sequence-id-213540

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  31. p(+)MV-NSE-FlagP-M502-3p

    Plasmid
    #58748
    Purpose
    Expresses recombinant measles virus with Flag-P gene tagged si2 (siP) target sequence
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Tags
    Flag
    Promoter
    T7
    Available Since
    Aug. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    58748-plasmid-map-sequence-id-90880

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  35. pAAV-S5E2-ChR2-mCherry (AAV1)

    Viral Prep
    #135634-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-S5E2-ChR2-mCherry (#135634). In addition to the viral particles, you will also receive purified pAAV-S5E2-ChR2-mCherry plasmid DNA. Expression of ChR2-mCherry under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jordane Dimidschstein
    Article
    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.
    Tags
    mCherry
    Available Since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only

  37. ZJOM65

    Plasmid
    #133654
    Purpose
    Integration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-VPH1-mCherry (VPH1 Budding Yeast)
    Tags
    mCherry
    Expression
    Yeast
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133654-plasmid-map-sequence-id-267529

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  43. pmScarlet3H-C1

    Plasmid
    #242975
    Purpose
    C1 Cloning vector for mammalian cells incorporating mScarlet3 -H red fluorescent protein
    Depositor
    Dorus Gadella
    Insert
    mScarlet3-H
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242975-plasmid-map-sequence-id-492373

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  44. snuABE4.2

    Plasmid
    #246014
    Purpose
    snuABE4.1 with MLH1-SB fused to the C-terminus via P2A.
    Depositor
    Sangsu Bae
    Article
    Im et al bioRxiv 2025.07.19.664718
    Insert
    Pediculus humanus ADAR deaminase domain (E438Q, I536S, E637F mutant)
    Tags
    MLH1-SB
    Expression
    Mammalian
    Mutation
    E438Q, I536S, E637F, with 10 amino acids truncati…
    Promoter
    CMV
    Available Since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    246014-plasmid-map-sequence-id-492308

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  45. snuABE4.1

    Plasmid
    #246013
    Purpose
    snuABE4.1 composed of PhADAR(E438Q, I536S, E637F) with 10a.a truncation fused to SpCas9 nickase [R221K, N394K, H840A] via an SV40 linker and further fused to La protein.
    Depositor
    Sangsu Bae
    Article
    Im et al bioRxiv 2025.07.19.664718
    Insert
    Pediculus humanus ADAR deaminase domain (E438Q, I536S, E637F mutant)
    Expression
    Mammalian
    Mutation
    E438Q, I536S, E637F, with 10 amino acids truncati…
    Promoter
    CMV
    Available Since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    246013-plasmid-map-sequence-id-492306

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  46. snuABE3.1

    Plasmid
    #246011
    Purpose
    snuABE3.1 composed of PhADAR(E438Q) fused to SpCas9 nickase [R221K, N394K, H840A] via an SV40 linker and further fused to La protein.
    Depositor
    Sangsu Bae
    Article
    Im et al bioRxiv 2025.07.19.664718
    Insert
    Pediculus humanus ADAR deaminase domain (E438Q mutant)
    Expression
    Mammalian
    Mutation
    E438Q
    Promoter
    CMV
    Available Since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    246011-plasmid-map-sequence-id-492301

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  47. pAG mIgG2a Anti-c-Myc [9E10]

    Plasmid
    #242955
    Purpose
    Mammalian expression plasmid for Anti-c-Myc [9E10] fused to mouse IgG2a heavy chain constant region; to be used with pAG mKappa Anti-c-Myc light chain [9E10] (Plasmid 242956) to make the antibody.
    Depositor
    Meghan Rego, Addgene Research Program
    Insert
    Anti-c-Myc [9E10] fused to mouse IgG2a heavy chain
    Tags
    Mouse IgG2a
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    242955-plasmid-map-sequence-id-484469

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  48. pAG mKappa Anti-c-Myc [9E10]

    Plasmid
    #242956
    Purpose
    Mammalian expression plasmid for Anti-c-Myc [9E10] fused to mouse kappa light chain constant region; to be used with pAG mIgG2a Anti-c-Myc [9E10] heavy chain (Plasmid 242955) to make the antibody.
    Depositor
    Meghan Rego, Addgene Research Program
    Insert
    Anti-c-Myc [9E10] light chain variable region fused to mouse kappa light chain constant region
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    242956-plasmid-map-sequence-id-485246

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Showing: 6701 - 6750 of 169450 results