167,601 results

1. pcDNA3.1-NINJ1

   Plasmid

   #201180

   Purpose

   Transient expression of NINJ1 gene in mammalian cells

   Depositor

   Jonathan Kagan

   Article

   Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

   Insert

   Ninjurin 1 (Ninj1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWZL-Blast FAPwt

   Plasmid

   #210643

   Purpose

   Expresses the FAP (Fibroblast Activation Protein) protein.

   Depositor

   Jing Yang

   Article

   Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

   Insert

   Fibroblast Activation Protein Alpha (FAP Human)
   

   Use

   Retroviral

   Mutation

   none

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF1a-BirA-V5-neo

   Plasmid

   #100548

   Purpose

   Expresses C-terminal V5-tagged BirA biotin ligase

   Depositor

   Jian Xu

   Article

   Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

   Insert

   C-terminal V5-tagged BirA
   

   Tags

   V5, His

   Expression

   Mammalian

   Promoter

   Human EF1a

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Human SAM library in lentiSAMv2 backbone

   Pooled Library

   #1000000078

   Purpose

   Genome-wide CRISPR gRNA pooled library for activation of human genes

   Depositor

   Feng Zhang

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

5. DsRed-rab11 WT

   Plasmid

   #12679

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB11A (Homo sapiens) (RAB11A Human)
   

   Tags

   DsRed and HA

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pROSA26-PA

   Plasmid

   #21271

   Depositor

   Frank Costantini

   Article

   Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

   Insert

   ROSA26 (Gt(ROSA)26Sor Mouse)
   

   Use

   Mouse Targeting

   Available Since

   Oct. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV enAsCas12f-HKRA-sgRNA_v7

   Plasmid

   #208357

   Purpose

   Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

   Depositor

   Atsushi Hoshino

   Article

   Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

   Insert

   enAsCas12f-HKRA
   

   Expression

   Mammalian

   Mutation

   I123H/D195K/D208R/V232A

   Promoter

   CMV

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2547

   Plasmid

   #144039

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CEBPD (CEBPD Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CaMKIIa-mCherry (AAV Retrograde)

   Viral Prep

   #114469-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   CaMKII

   Tags

   mCherry

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

10. paavCAG-iCre

    Plasmid

    #51904

    Purpose

    For AAV expression of iCre recombinase

    Depositor

    Jinhyun Kim

    Article

    Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

    Insert

    improved Cre recombinase
    

    Use

    AAV and Cre/Lox

    Promoter

    CAG

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ152

    Plasmid

    #69303

    Purpose

    Gateway entry vector with pco-dCas9-VP64

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pco-dCas9-VP64 (Plant codon-optimized)
    

    Use

    CRISPR

    Mutation

    VP64 activator fusion; NLS fusion

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMEH303 NLS dTomato 6x high affinity MS2 loops

    Plasmid

    #168215

    Purpose

    Mammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loops

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    NLS dTomato
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. ABE8e(TadA-8e V106W)

    Plasmid

    #138495

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e V106W)-nSpCas9
    

    Expression

    Mammalian

    Mutation

    TadA-8e V106W

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDualIN-SMN2

    Plasmid

    #214430

    Purpose

    Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN2 EXON 7 (SMN2 Human)
    

    Expression

    Mammalian

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF3007

    Plasmid

    #144483

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SMAD1 (SMAD1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. mito-dendra2

    Plasmid

    #55796

    Purpose

    photo-activatable mitochondrial marker for expression in mammalian cells

    Depositor

    David Chan

    Article

    Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

    Insert

    mito-Dendra2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. KARS_pNIC-Bio3

    Plasmid

    #153051

    Purpose

    Expression construct for producing Avi tagged Lysyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    KARS (KARS1 Human)
    

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. FH-TET3-pEF

    Plasmid

    #49446

    Purpose

    This plasmid expresses full-length human TET3.

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    Tet methylcytosine dioxygenase 3 (TET3 Human)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV Myc T58A puro

    Plasmid

    #18773

    Depositor

    Scott Lowe

    Article

    Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

    Insert

    myc T58A (MYC Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T58A

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-GRAB-gDA3h

    Plasmid

    #208699

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_gDA3h
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV_hSyn-DIO-PdCO-mScarlet-WPRE

    Plasmid

    #198511

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Has Service

    AAV1 and AAV5

    Insert

    PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pSW002-Pc-mTurquoise2

    Plasmid

    #108235

    Purpose

    Broad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    Pc promoter - mTurquoise2
    

    Expression

    Bacterial

    Mutation

    codon optimized for experession in P. fluorescens

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. ERM-APEX2

    Plasmid

    #79055

    Purpose

    Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    APEX2 anchored to the cytosolic face of the ER membrane
    

    Use

    Lentiviral

    Tags

    Residues 1-27 of P450 oxidase 2C1 and V5

    Expression

    Bacterial and Mammalian

    Promoter

    CMV immearly promotor

    Available Since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. NeonGreen-C1-ORP5

    Plasmid

    #220071

    Purpose

    overexpresses ORP5 with a neonGreen fluorophore

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    OSBPL5 (OSBPL5 Human)
    

    Expression

    Mammalian

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-HDAC2-H141A

    Plasmid

    #115346

    Purpose

    Expresses mouse HDAC2 cDNA with H141A point mutation

    Depositor

    Javier Gonzalez-Maeso

    Article

    Kurita et al Nat Neurosci. 2012 Sep;15(9):1245-54. do

    Insert

    Histone deacetylase 2 (Hdac2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H141A

    Promoter

    CMV

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. mEos3.2-C1

    Plasmid

    #54550

    Purpose

    Localization: C1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Tao Xu, Michael Davidson

    Article

    Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

    Type

    Empty backbone

    Tags

    mEos3.2

    Expression

    Mammalian

    Available Since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. Paxillin-pEGFP

    Plasmid

    #15233

    Depositor

    Rick Horwitz

    Article

    Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

    Insert

    paxillin (PXN Chicken)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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28. CD44S pBabe-puro

    Plasmid

    #19127

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    CD44 standard (CD44 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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29. pVDL9.3

    Plasmid

    #168299

    Purpose

    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

    Depositor

    Víctor de Lorenzo

    Article

    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

    Insert

    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    

    Expression

    Bacterial

    Promoter

    LacI-Plac

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pET15b His6-FKBPF36V

    Plasmid

    #73180

    Purpose

    Expressing FKBPF36V protein in washing out shield-1 ligand for FKBPdd

    Depositor

    Thomas Wandless

    Article

    Egeler et al J Biol Chem. 2011 Sep 9;286(36):31328-36

    Insert

    FKBP(F36V) (FKBP1A Human)
    

    Tags

    His 6x

    Expression

    Bacterial

    Mutation

    F36V

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

31. pDONR223-ERBB4

    Plasmid

    #23875

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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32. pcDNA5/FRT/TO-GAlphaQ-RLuc8

    Plasmid

    #140982

    Purpose

    Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaQ-RLuc8 (GNAQ Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Inzolia library in pRDA_052

    Pooled Library

    #209551

    Purpose

    The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

    Depositor

    John Doench

    Article

    Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

34. pBPnlsLexA::GADflUw

    Plasmid

    #26232

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    nlsLexA::GADfl
    

    Expression

    Insect

    Available Since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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35. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (AAV9)

    Viral Prep

    #100054-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (#100054). In addition to the viral particles, you will also receive purified pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 plasmid DNA. CAG-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    CAG

    Tags

    mCherry

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

36. pLenti-CMV-MCS-GFP-SV-puro

    Plasmid

    #73582

    Purpose

    Third generation lentiviral vector expressing GFP and puromycin

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP tag

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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37. CAV1-mCherry

    Plasmid

    #27705

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    caveolin-1 (CAV1 Canis lupus familiaris)
    

    Tags

    monomeric Cherry

    Expression

    Mammalian

    Available Since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pC1-HyPerRed-mito

    Plasmid

    #60247

    Purpose

    Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HyPerRed-mito
    

    Tags

    Mitochondrial targeting signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLVX-Bsd-CMV-MDM4

    Plasmid

    #202669

    Purpose

    MDM4 overexpression vector with blasticidin resistance

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 regulator of p53 (MDM4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAdTrack-CMV FLAG Rev-Erb alpha

    Plasmid

    #22745

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    Rev Erb alpha (NR1D1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Jan. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge