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pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST

Plasmid

#175020

Purpose

Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

Depositor

Robert de Vries

Article

Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

Insert

D/bovine/Nebraska/9-5/2012

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

s57a esterase knock out mutant

Promoter

CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJ3 SHP2 D61A

Plasmid

#8320

Depositor

Ben Neel

Article

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Expression

Mammalian

Mutation

D61A (activated mutant)

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
FLAG-ARP8

Plasmid

#15361

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

actin-related protein 8 (ACTR8 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pSNV2 CHER NLS cop1at(335-675)

Plasmid

#44972

Depositor

Thanos Halazonetis

Article

Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

Insert

mCherry-NLS-COP1 (COP1 Mustard Weed)

Tags

NLS and mCherry

Expression

Mammalian

Mutation

deleted amino acids 1-334

Promoter

pCMV IE

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.PAC

Plasmid

#57829

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/pMXs-IG

Plasmid

#51258

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Insert

3xFLAG-dCas9

Use

CRISPR and Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pTracer_Cr-TRP1

Plasmid

#64881

Purpose

Expresses CrTRP1 in mammalian cells

Depositor

Sebastián Brauchi

Article

Arias-Darraz et al Plant Cell. 2015 Jan;27(1):177-88. doi:

Insert

Transient receptor potential 1

Tags

eGFP

Promoter

CMV

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-Sestrin3

Plasmid

#72592

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN3 (SESN3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only
pAG70 BDNF 3'UTR

Plasmid

#12021

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

BDNF 3'UTR (BDNF Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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Cas12f-GE ver4.0

Plasmid

#176543

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Article

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Inserts

Cas12f-GE ver4.0
Cas12f-GE ver4.0

Expression

Mammalian

Promoter

U6 and chicken β-actin

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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Dynamin-I-K44A-6His/Flag

Plasmid

#32696

Depositor

Robert Lefkowitz

Article

Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

Insert

Dynamin I (Dnm1 Rat)

Tags

Flag and His6

Expression

Mammalian

Mutation

K44A

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pBabe puro RalA Q75L

Plasmid

#19719

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

GTPase deficient & constitutively active Gl…

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pBJ5 BRG1 DN

Plasmid

#17874

Depositor

Jerry Crabtree

Article

Khavari et al Nature. 1993 Nov 11. 366(6451):170-4.

Insert

Brg DN (SMARCA4 Human)

Expression

Mammalian

Mutation

dominant negative

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
YF-Cdc42(CA)

Plasmid

#20152

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Cdc42(CA) (CDC42 Human)

Expression

Mammalian

Mutation

Cdc42H was inserted into the multiple cloning sit…

Available Since

March 26, 2009

Availability

Academic Institutions and Nonprofits only

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pX330.Pten.b

Plasmid

#66588

Purpose

sgRNA for Pten deletion

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

Pten deletion (Pten Mouse)

Expression

Mammalian

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

Plasmid

#174892

Purpose

amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag

Plasmid

#199457

Purpose

Expression of dCas13-VPR in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

dCas13

Use

Lentiviral

Tags

VPR-T2A-HaloTag

Expression

Mammalian

Mutation

Nuclease-dead H133A and H1058A

Promoter

CAG

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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MSP1594_2x_NLS

Plasmid

#110625

Purpose

Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
None

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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CDA1-BE3

Plasmid

#100804

Purpose

Expresses CDA1-BE3 in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

CDA1-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_ORF9B_HA_BASU

Plasmid

#153981

Purpose

Express Orf9b gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

orf9b

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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CABE-T2.19

Plasmid

#193265

Purpose

Expression of CABE-T2.19 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T2.19

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-hKLF4 (Plath)

Plasmid

#17967

Depositor

Kathrin Plath

Article

Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

Kruppel-like factor 4 (gut) (KLF4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

N/A

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pAGH10 - RlucFluc minus 1 HIV-1

Plasmid

#198224

Purpose

Fluorescence reporters for -1 ribosomal frameshifting in mammalian cells

Depositor

Isha Jain

Article

Baik et al Mol Cell. 2023 Mar 16;83(6):942-960.e9.

Inserts

Renilla luciferase
Firefly luciferase

Expression

Mammalian

Promoter

SV40

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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Cas12f-GE ver3.0

Plasmid

#176508

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Article

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Inserts

Cas12f-GE ver3.0
Cas12f-GE ver3.0

Expression

Mammalian

Promoter

U6 and chicken β-actin

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2

Plasmid

#113890

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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SPLINTR GFP v1 backbone

Plasmid

#179777

Purpose

SPLINTR backbone vector

Depositor

Mark Dawson

Article

Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_eA3A-nCas9

Plasmid

#163535

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX

Plasmid

#188991

Purpose

A plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

F163L, K249R, G328S, G381V, G429D, D480G

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Sa-dCas9-NLS-3xFLAG/pcDNA3.1

Plasmid

#98041

Purpose

Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

Sa-dCas9-NLS-3xFLAG

Use

CRISPR

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

D10A + H557A

Promoter

CMV

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ3 SHP2 CS

Plasmid

#8319

Depositor

Ben Neel

Article

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Expression

Mammalian

Mutation

C459S, mutation of essential catalytic cysteine

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG HDAC5 S259A/S498A

Plasmid

#32216

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser259Ala, Ser498Ala

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pAAV_GWRev_Dest

Plasmid

#86954

Purpose

Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. With cre, insert will flip backwards, causing it to turn off (cre-off)

Depositor

Joseph Dougherty

Article

Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
DCT.VV.Orf170

Plasmid

#12858

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf170

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pCMV6M-Pak1 H83L H86L

Plasmid

#12211

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

PBD (p21-binding domain) mutant: H83L, H86L (see …

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-hA3F-BE3

Plasmid

#113414

Purpose

Expresses hA3F-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3F-BE3 (APOBEC3F S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPRv1_UQCRC2

Plasmid

#177982

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting UQCRC2

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting UQCRC2 (UQCRC2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-haA3A-CBE-VA

Plasmid

#220893

Purpose

Express haA3A-CBE-VA (with SpCas9) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-VA

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-haA3A-CBE-VA-NG

Plasmid

#220896

Purpose

Express haA3A-CBE-VA-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-VA-NG

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

Plasmid

#174891

Purpose

amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-hSOX2 (Plath)

Plasmid

#17965

Depositor

Kathrin Plath

Article

Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

SRY (sex determining region Y)-box 2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

N/A

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
MDH1-miR-31-PGK-GFP-Blast

Plasmid

#31515

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-31 (Mir31 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pRK5 GFP RagB Q99L

Plasmid

#112749

Purpose

express GFP-RagB (Q99L mutant)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagB (Q99L) (RRAGB Human)

Tags

eGFP

Expression

Mammalian

Mutation

Q99L

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_GW_Dest

Plasmid

#86955

Purpose

Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. Without cre, insert is backwards in reference to promoter.

Depositor

Joseph Dougherty

Article

Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe puro RalA S31N

Plasmid

#19718

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

S31N mutant for dominant neg function. NOTE: lo…

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf45

Plasmid

#12733

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf45

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3 Ub2-UBB+1

Plasmid

#23968

Depositor

Nico Dantuma

Article

Lindsten et al J Cell Biol. 2002 Apr 29. 157(3):417-27.

Insert

Ubiquitin B +1 transcript (UBB Human)

Expression

Mammalian

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEx-mCherry-LINXa3

Plasmid

#73614

Purpose

Light-induced nuclear export switch for microscopy in mammalian cells.

Depositor

Brian Kuhlman

Article

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

LINXa3

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

Chicken B-Actin

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SARS_COV2_ORF3b_HA_BASU

Plasmid

#153992

Purpose

Express Orf3b gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

orf3b

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-dCpf1-BE-YE

Plasmid

#107686

Purpose

Expresses dLbCpf1-BE-YE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE-YE

Expression

Mammalian

Mutation

APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

Promoter

CMV

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo

Plasmid

#78352

Purpose

Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-KRAB

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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