Skip to main content
Addgene
Showing: 901 - 950 of 159683 results

  8. DT960

    Plasmid
    #80412
    Purpose
    Expresses 960 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
    Depositor
    Thomas Cooper
    Article
    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub
    Insert
    human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80412-plasmid-map-sequence-id-151397

    Enlarge

  10. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid
    #73440
    Purpose
    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE-P4A6-vioC-P3A2-vioD
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73440-plasmid-map-sequence-id-135339

    Enlarge

  14. pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (AAV1)

    Viral Prep
    #83897-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (#83897). In addition to the viral particles, you will also receive purified pAAV-hDlx-GqDREADD-dTomato-Fishell-4 plasmid DNA. hDlx-driven expression of GqDREADD (hM3D) and nuclear localized dTomato (physically separate) for activation of interneurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    hDlx
    Tags
    dTomato
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV-hSyn-GRAB_OT1.0 (AAV1)

    Viral Prep
    #185386-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-GRAB_OT1.0 (#185386). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_OT1.0 plasmid DNA. hSyn-driven expression of the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Promoter
    Syn
    Tags
    None
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  27. Lenti-LucOS

    Plasmid
    #22777
    Depositor
    Tyler Jacks
    Insert
    OVA-SIY
    Use
    Cre/Lox and Lentiviral
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    22777-plasmid-map-sequence-id-419746

    Enlarge

  33. pFC334

    Plasmid
    #87846
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    argB
    yA specific sgRNA
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    A. nidulans gpdA promoter with Hammerhead ribozym…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87846-plasmid-map-sequence-id-174613

    Enlarge

  35. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid
    #50831
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    Use
    Baculoviral
    Tags
    His, Myc-6xHis, and WPRE
    Expression
    Insect
    Mutation
    Y39TAG and able to charge various unnatural amino…
    Promoter
    CAG, CMV, and U6 and H1
    Available since
    June 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    50831-plasmid-map-sequence-id-193569

    Enlarge

  38. GPR52-Tango

    Plasmid
    #66362
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR52 (GPR52 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  40. pMBI

    Plasmid
    #17816
    Depositor
    Jay Keasling
    Article
    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802
    Insert
    mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 28, 2008
    Availability
    Academic Institutions and Nonprofits only

  46. AICSDP-36: AAVS1-mEGFP (PGK)

    Plasmid
    #114404
    Purpose
    Homology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP
    Depositor
    Allen Institute for Cell Science
    Insert
    AAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114404-plasmid-map-sequence-id-220815

    Enlarge

Showing: 901 - 950 of 159683 results