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Addgene

We narrowed to 18,777 results for: IRE

Showing: 9801 - 9850 of 18777 results
  1. pcDNA5/FRT/TO/SH/GW-Mst1

    Plasmid
    #84299
    Purpose
    Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.
    Depositor
    Insert
    Mst1 (STK4 Human)
    Use
    Tags
    HA, SBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pRAN357

    Plasmid
    #120898
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein
    Insert
    cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptet
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pGEX-2T-FRB-W2027F

    Plasmid
    #73341
    Purpose
    Point mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTOR
    Depositor
    Insert
    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    Use
    Tags
    GST-tagged
    Expression
    Bacterial
    Mutation
    FRB domain only aa2015-2114; W2027F
    Promoter
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
  4. pLVX-TetOneZeo-ORF18(E36A_L37A)-3xFLAG

    Plasmid
    #129758
    Purpose
    Dox inducible expression of KSHV ORF18-3xFLAG (E36A_L37A) in mammalian cells
    Depositor
    Insert
    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    changed Glutamic acid 36 to Alanine and Leucine 3…
    Promoter
    TRE3Gs and Tet inducible promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pGEX-2T FRB Y2105A

    Plasmid
    #68726
    Purpose
    Point mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTOR
    Depositor
    Insert
    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    Use
    Tags
    GST-tagged
    Expression
    Bacterial
    Mutation
    FRB domain only aa2015-2114; Y2105A
    Promoter
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
  6. pNB785

    Plasmid
    #60164
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.
    Depositor
    Insert
    FLuc-yEVenus-PEST
    Use
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    SIC1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
  7. pNB787

    Plasmid
    #60152
    Purpose
    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.
    Depositor
    Insert
    FLuc-yEVenus-PEST
    Use
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    LEU1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. GST-PC-2-CTS

    Plasmid
    #86068
    Purpose
    A bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.
    Depositor
    Insert
    Polycystin-2 (Pkd2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    contains the region from 1-15
    Promoter
    tac
    Available since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. pNB789

    Plasmid
    #60166
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.
    Depositor
    Insert
    FLuc-yEVenus-PEST
    Use
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    RNR1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 9801 - 9850 of 18777 results