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We narrowed to 3,815 results for: LYC;

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Showing: 41 - 80 of 3815 results

  2. SARS-CoV-2 S HexaPro

    Plasmid
    #154754
    Purpose
    Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant
    Depositor
    Jason McLellan
    Article
    Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505
    Insert
    Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Available Since
    July 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154754-plasmid-map-sequence-id-304640

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  9. pcDNA3-SARS-CoV-2-S-RBD-8his

    Plasmid
    #145145
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    Tags
    8his tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145145-plasmid-map-sequence-id-324821

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  11. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

    Plasmid
    #103871
    Purpose
    dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103871-plasmid-map-sequence-id-259670

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  12. pLV-dCas9-KRAB-PGK-HygR

    Plasmid
    #83890
    Purpose
    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Mutation
    D10A and H840A
    Promoter
    Human Ubiquitin C Promoter and mouse phosphoglyce…
    Available Since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83890-plasmid-map-sequence-id-240902

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  15. GPD-roGFP

    Plasmid
    #49436
    Purpose
    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells
    Depositor
    Paul Schumacker
    Article
    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi
    Insert
    GPD-roGFP2
    Use
    Adenoviral
    Tags
    Glycerol phosphate dehydrogenase, 5' to roGF…
    Expression
    Mammalian
    Mutation
    C48S, Q80R, S147C, and Q204C in Clontech's G…
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49436-plasmid-map-sequence-id-251833

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  16. pcDNA3-SARS-CoV-2-S-RBD-Fc

    Plasmid
    #141183
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and S…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141183-plasmid-map-sequence-id-288058

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  17. pPGK-Cre-bpA

    Plasmid
    #11543
    Purpose
    Expresses Cre recombinase from mouse PGK promoter in mammalian cells.
    Depositor
    Klaus Rajewsky
    Insert
    Phosphoglycerate kinase 1 promoter
    Use
    Cre/Lox
    Expression
    Mammalian
    Promoter
    mouse PGK
    Available Since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only
    11543-plasmid-map-sequence-id-6971

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  18. pET29a-YS14

    Plasmid
    #66890
    Purpose
    Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)
    Depositor
    Jung-Hyun Min
    Article
    Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.
    Inserts
    His6-Thrombin-Xenopus laevis histones H2A
    Xenopus laevis histones H2B
    Xenopus laevis histones H3
    Xenopus laevis histones H4-Thrombin
    Tags
    H2A, H2B, H3, H4, His6 tag, and Thrombin
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    66890-plasmid-map-sequence-id-305064

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  22. pMA3790

    Plasmid
    #70110
    Purpose
    mtDNA elimination/production of rho-0 cells
    Depositor
    Mikhail Alexeyev
    Article
    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi
    Inserts
    EGFP
    hUNG1 Y147A
    Neo
    Tags
    None, human OTC mitochondrial targeting sequence,…
    Expression
    Mammalian
    Mutation
    Y147A, aa's 1-77 removed
    Promoter
    CMV, EF1a, and SV40 and native
    Available Since
    May 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    70110-plasmid-map-sequence-id-399654

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  33. pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

    Plasmid
    #103870
    Purpose
    High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    103870-plasmid-map-sequence-id-199178

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  35. lentiCRISPR v2-dCas9

    Plasmid
    #112233
    Purpose
    Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2
    Depositor
    Thomas Gilmore
    Article
    Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do
    Inserts
    dCas9
    Porumycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Changed: Aspartic acid 10 to Glycine, Histidine 8…
    Promoter
    EFS-NS
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112233-plasmid-map-sequence-id-213540

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  40. pAAV-hSyn-GRAB_NE1m

    Plasmid
    #123308
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons
    Depositor
    Yulong Li
    Article
    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d
    Has Service
    AAV Retrograde and AAV9
    Insert
    GPCR activation based NE sensor GRAB_NE1m
    Use
    AAV
    Mutation
    contains a glycine-to-threonine mutation at posit…
    Promoter
    hSyn
    Available Since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    123308-plasmid-map-sequence-id-240666

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Showing: 41 - 80 of 3815 results