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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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169,450 results

Showing: 10001 - 10050 of 169450 results

  1. pBJ2

    Plasmid
    #167131
    Purpose
    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter
    Depositor
    Akira Mine
    Article
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Expression
    Bacterial
    Promoter
    Kanamycin promoter
    Available Since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167131-plasmid-map-sequence-id-330437

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  2. pAcGFP-N1_CBP

    Plasmid
    #221903
    Purpose
    Transient expression of GFP-tagged CBP in mammalian cells
    Depositor
    Daniel Bose
    Insert
    CBP (CREBBP Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    N/A
    Available Since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    221903-plasmid-map-sequence-id-437447

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  4. PB-CMV-MCS-Puro-EGFP

    Plasmid
    #219795
    Purpose
    Mammalian expression plasmids using piggyBac Transposon System
    Depositor
    Keiichi Nakayama
    Type
    Empty backbone
    Use
    Piggybac transposon
    Expression
    Mammalian
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    219795-plasmid-map-sequence-id-441250

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  8. pME-dCas9-BFP-KRAB

    Plasmid
    #221502
    Purpose
    CRISPRi machinery fish codon optimized
    Depositor
    Alessandro Ori
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Mutation
    D10A and H839A point mutations
    Available Since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221502-plasmid-map-sequence-id-439413

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  18. pNL [NlucP/TCF/LEF-RE/Hygro] Vector

    Plasmid
    #236824
    Purpose
    Express LEF response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    LEF
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Mutation
    None
    Promoter
    minP
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236824-plasmid-map-sequence-id-471826

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  22. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Expression
    Mammalian
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

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  23. pAAV-hSyn-EGFP (AAV11 trial size)

    Viral Prep
    #50465-AAV11.T
    Purpose
    Ready-to-use AAV11 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only

  26. hSYN-mito-dsRED2

    Plasmid
    #173069
    Purpose
    mitochondria targeted dsRED2 expressed off of human Synapsin I promoter.
    Depositor
    Thomas Schwarz
    Insert
    Mito
    Use
    Lentiviral
    Tags
    dsRED
    Expression
    Mammalian
    Promoter
    human synapsin 1
    Available Since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    173069-plasmid-map-sequence-id-345051

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  27. TFORF3132

    Plasmid
    #144608
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ESR2 (ESR2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144608-plasmid-map-sequence-id-346764

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  30. TFORF2744

    Plasmid
    #144225
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    T (TBXT Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144225-plasmid-map-sequence-id-404099

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  39. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197026
    Purpose
    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized enOsCas12f1
    Expression
    Mammalian
    Mutation
    D52R / T132R
    Promoter
    CBh, CMV, hU6
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197026-plasmid-map-sequence-id-394112

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  40. TFORF0983

    Plasmid
    #143739
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KLF5 (KLF5 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143739-plasmid-map-sequence-id-407888

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  42. pDisplay-GRAB_HTR6-1.0

    Plasmid
    #180005
    Purpose
    Expresses the 5-HT sensor GRAB_HTR6-1.0 in mammalian cells
    Depositor
    Yulong Li
    Insert
    5-HT sensor GRAB_HTR6-1.0
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    180005-plasmid-map-sequence-id-358496

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  46. pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry

    Plasmid
    #203652
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire-IRES-H2B-mCherry
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203652-plasmid-map-sequence-id-402483

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  50. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV PHP.eB)

    Viral Prep
    #20298-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available Since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 10001 - 10050 of 169450 results