163,398 results

1. gRNA_Cloning Vector

   Plasmid

   #41824

   Purpose

   An empty gRNA expression vector, used to create a gRNA to a specific sequence.

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   AflII site for Gibson Assembly

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPath-Violacein-pT7native

   Plasmid

   #200002

   Purpose

   Violacein pathway. pT7-native CDS sequence

   Depositor

   Farren Isaacs

   Article

   Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

   Insert

   Pathways
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ACER

   Pooled Library

   #226117

   Purpose

   The Advanced Catalogue of Epigenetic Regulators (ACER) library allows scientists to study how different epigenetic proteins influence gene activity and contribute to diseases.

   Depositor

   Rui Lu

   Article

   Zhou et al Blood. 2024 Aug 16:blood.2023023644. doi

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

4. Anti-Biotin [IPI-M33] (Recombinant Antibody)

   Antibody

   #218102-rAb

   Purpose

   Anti-Biotin chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to biotin.

   Depositor

   Institute for Protein Innovation (IPI)

   Article

   Institute for Protein et al Unpublished

   Recommended Applications

   Western Blot

   Reactivity

   Synthetic

   Source Species

   Rabbit

   Isotype

   IgG1

   Trial Size

   Not available to purchase

   Available since

   May 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

5. pLV-EF1A-CD19.8h.8TM.28.3z

   Plasmid

   #200670

   Purpose

   Modular CD8hinge-CD8transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD8HD-CD8TM-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENN.AAV.CMVs.Pl.Cre.rBG (AAV rh10)

   Viral Prep

   #105537-rh10

   Purpose

   Ready-to-use AAV rh10 (similar to AAV10) particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   CMV

   Tags

   None

   Available since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

7. Desmoglein2-mCherry

   Plasmid

   #36991

   Depositor

   Kathleen Green

   Article

   Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

   Insert

   Dsg2 (DSG2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMXs-Oct3/4

   Plasmid

   #13366

   Purpose

   Retroviral expression of mouse Oct3/4

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1 Dendra2

   Plasmid

   #103967

   Purpose

   Mammalian expression of fluorescent protein Dendra2 used for transfection efficiency control

   Depositor

   Qing Deng

   Article

   Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

   Insert

   Dendra2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUCP20T-dsRedExpress

    Plasmid

    #78470

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    dsRedExpress
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti PGK Blast DEST (w524-1)

    Plasmid

    #19065

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMSCV-mLin28A

    Plasmid

    #26357

    Depositor

    George Daley

    Article

    Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

    Insert

    lin-28 homolog A (Lin28a Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pRK5-FLAG-Sestrin2

    Plasmid

    #72595

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN2 (SESN2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1 mycBioID

    Plasmid

    #35700

    Purpose

    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    cmv

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHCMV-LCMV-WE

    Plasmid

    #15793

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    Lymphocytic choriomeningitis virus glycoprotein LCMVGP gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR221-NR1H2

    Plasmid

    #79513

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H2 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    LXR BETA (NR1H2 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS416-yZ3EV-Z3pr (RB3578)

    Plasmid

    #69099

    Purpose

    URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr

    Depositor

    David Botstein

    Inserts

    yZ3EV cassette
    Z3 promoter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-TLR7-YFP

    Plasmid

    #13022

    Depositor

    Doug Golenbock

    Insert

    TLR7 (TLR7 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Bassik lab Human CRISPR-Cas9 Deletion Library - Apoptosis and cancer

    Pooled Library

    #101926

    Purpose

    Bassik lab Human CRISPR-Cas9 Deletion Library - Apoptosis and cancer. Coexpresses mCherry.

    Depositor

    Michael Bassik

    Article

    Morgens et al Nat Commun. 2017 May 5;8:15178. doi: 10.

    Expression

    Mammalian

    Use

    CRISPR

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

20. mNeonGreen-APEX3

    Plasmid

    #202590

    Purpose

    Gamma-retroviral vector that expresses mNeonGreen fused to APEX3.

    Depositor

    Reuben Harris

    Article

    Becker et al J Mol Biol. 2023 May 12:168145. doi: 10.

    Insert

    mNeonGreen-APEX3-V5 (APX1 )
    

    Use

    Retroviral

    Tags

    V5 epitope and mNeonGreen

    Expression

    Mammalian

    Mutation

    APEX2 L242A

    Promoter

    MESV LTR

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ACE2

    Plasmid

    #164219

    Purpose

    Expresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection marker

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    silent mutation to remove internal EcoRI site (GA…

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (AAV8)

    Viral Prep

    #137141-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (#137141). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of ChR2(E123T/T159C)-EYFP (inhibited in presence of Cre) for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    EYFP (Flp-dependent)

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

23. pAR357_SFP_pCDF-1b

    Plasmid

    #122851

    Purpose

    Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    Sfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAX FLAG APP

    Plasmid

    #30154

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wild type splice variant 751

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pOpen-T4 PNK

    Plasmid

    #165520

    Purpose

    The most commonly used polynucleotide kinase.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    T4 PNK
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pRK5-FLAG-USP5

    Plasmid

    #61742

    Purpose

    express N-terminally FLAG tagged human USP5

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    USP5 (USP5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCDH-PGK

    Plasmid

    #72268

    Purpose

    Express gene of interest under PGK (phosphoglycerate kinase I) promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pHAGE-CDK12

    Plasmid

    #116723

    Purpose

    Lentiviral expression of CDK12

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    CDK12 (CDK12 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR221-SLC5A5_STOP

    Plasmid

    #161348

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC5A5 (SLC5A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pINDUCER dCas9-TET1CDMut

    Plasmid

    #101920

    Purpose

    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.

    Depositor

    Danwei Huangfu

    Article

    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

    Insert

    dCas9-TET1CD Mut
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Mutated TET1 catalytic domain

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge