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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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171,853 results

Showing: 11701 - 11750 of 171853 results

  8. psd44-Lactadherin46

    Plasmid
    #46830
    Purpose
    expression of human lactadherin in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    MFGE8 (MFGE8 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    PGK promoter
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    46830-plasmid-map-sequence-id-410396

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  9. NEDD4

    Plasmid
    #25141
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    nedd4.0001.0152 (NEDD4 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. pBBR1.cas9-NT

    Plasmid
    #197871
    Purpose
    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)
    Depositor
    Baojun Wang
    Article
    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72
    Inserts
    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    Expression
    Bacterial
    Available Since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197871-plasmid-map-sequence-id-396456

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  12. pAD-SPS6-Pman

    Plasmid
    #133906
    Purpose
    Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.
    Depositor
    Oscar Kuipers
    Article
    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57
    Insert
    sfGFP(SPS6)
    Use
    E.coli/bacillus spp. shuttle vector
    Expression
    Bacterial
    Promoter
    Pman
    Available Since
    Nov. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    133906-plasmid-map-sequence-id-261727

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  14. pHREAC

    Plasmid
    #134908
    Purpose
    Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.
    Depositor
    George Lomonossoff
    Article
    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    134908-plasmid-map-sequence-id-342556

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  15. pT3EF1aH-myr-Akt

    Plasmid
    #179909
    Purpose
    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.
    Depositor
    Xin Chen
    Article
    Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:
    Insert
    Akt1 (Akt1 Mouse)
    Tags
    HA and Myr
    Expression
    Mammalian
    Available Since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    179909-plasmid-map-sequence-id-357764

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  20. pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

    Plasmid
    #128386
    Purpose
    Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption
    Depositor
    Craig Duvall
    Article
    Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss
    Inserts
    NLuc-3xGGGGS-Gal8-NCRD (LGALS8 Human, Synthetic)
    Gal8-NCRD-3xGGGGS-CLuc398 (LGALS8 Human)
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    C-terminal luc2 fragment and N-terminal luc2 frag…
    Expression
    Mammalian
    Mutation
    N terminal carbohydrate recognition domain fused …
    Promoter
    EF1A and IRES
    Available Since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    128386-plasmid-map-sequence-id-263327

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  21. NanoBRET MLL1/WDR5 Assay Donor Vector, MLL1

    Plasmid
    #237139
    Purpose
    Express MLL1-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    MLL1 (KMT2A Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237139-plasmid-map-sequence-id-473338

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  27. Anti-V5 [IPI-SV5-Pk1] (Recombinant Antibody)

    Antibody
    #218107-rAb
    Purpose
    Anti-V5 tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to GKPIPNPLLGLDST sequence.
    Depositor
    Institute for Protein Innovation (IPI)
    Article
    Randall et al J Gen Virol. 1987 Nov;68 ( Pt 11):2769-8
    Recommended Applications
    Western Blot
    Source Species
    Rabbit
    Isotype
    IgG
    Trial Size
    Not available to purchase
    Available Since
    May 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by Institute for Protein Innovation (IPI)

  28. pINDUCER20_mStrawberry_Atg4BC74A

    Plasmid
    #161735
    Purpose
    Doxycycline-inducible expression of Atg4B(C74A) fused with mStrawberry to inhibit autophagy
    Depositor
    Alec Kimmelman
    Article
    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:
    Insert
    Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    mStrawberry
    Expression
    Mammalian
    Mutation
    220-222 TGC (Cys) is changed to GCA (Ala)
    Available Since
    Dec. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161735-plasmid-map-sequence-id-320115

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  33. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid
    #197502
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197502-plasmid-map-sequence-id-386612

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  39. pPD96_32

    Plasmid
    #1504
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, ATG-MitLS, gfpN, IVSR, gfpS65C, IVSS, IVST,…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1504-plasmid-map-sequence-id-115

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  47. TFORF1974

    Plasmid
    #143988
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TBX3 (TBX3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143988-plasmid-map-sequence-id-385902

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Showing: 11701 - 11750 of 171853 results