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169,450 results

Showing: 11701 - 11750 of 169450 results

  2. SpyTag-sfGFP

    Plasmid
    #184226
    Purpose
    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpyTag-sfGFP
    Tags
    His6, SpyTag, and TEV protease cleavage site
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184226-plasmid-map-sequence-id-361605

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  6. L4857 dsRedExpress2 reporter, VEGFR2 NTEVp, VEGFR1 CTEVp in PiggyBac Transposon Vector

    Plasmid
    #244187
    Purpose
    PiggyBac transposon vector for expression of dsRed-Express2 synTF promoter; constitutive expression of VEGFR2 NTEVp chain, VEGFR1 CTEVp chain, and mNeonGreen-P2A-PuroR selection marker
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    dsRed-Express2 under synTF responsive promoter; VEGFR2 NTEVp chain with WT NTEVp; VEGFR1 CTEVp chain; mNeonGreen-P2A-PuroR
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244187-plasmid-map-sequence-id-489486

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  7. HaloTag-STAG1 Vector

    Plasmid
    #238689
    Purpose
    Express HaloTag-STAG1 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    STAG1 (STAG1 Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238689-plasmid-map-sequence-id-479895

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  8. HaloTag-RAD21 Vector

    Plasmid
    #238693
    Purpose
    Express HaloTag-RAD21 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    RAD21 (RAD21 Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238693-plasmid-map-sequence-id-479889

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  9. HaloTag-STAG2 Vector

    Plasmid
    #238685
    Purpose
    Express HaloTag-STAG2 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    STAG2 (STAG2 Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238685-plasmid-map-sequence-id-479924

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  23. pME-Cas9-T2A-GFP

    Plasmid
    #63155
    Purpose
    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.
    Depositor
    Leonard Zon
    Article
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Insert
    Cas9-T2A-GFP
    Use
    CRISPR; Tol2 middle entry vector for gateway
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    63155-plasmid-map-sequence-id-220487

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  25. eGFP-hCOL1A1

    Plasmid
    #140110
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag, K20…
    Promoter
    CMV
    Available Since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140110-plasmid-map-sequence-id-276323

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  34. pCBL101.1

    Plasmid
    #199724
    Purpose
    A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene. BsaI site was removed.
    Depositor
    Kan Wang
    Article
    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:
    Insert
    Multiple Cloning Site, NPTII
    Expression
    Plant
    Mutation
    "GAGACC" to "GATACC"
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199724-plasmid-map-sequence-id-391213

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  35. pGWB402SN

    Plasmid
    #224551
    Purpose
    Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.
    Depositor
    Paul Schulze-Lefert
    Article
    Lawson et al bioRxiv 2024.07.14.603419
    Type
    Empty backbone
    Tags
    HA-Twin-Strep
    Expression
    Plant
    Promoter
    2 X 35S
    Available Since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    224551-plasmid-map-sequence-id-444736

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  36. pDONR221-FURIN

    Plasmid
    #158442
    Purpose
    Gateway Cloning compatible entry vector for the human FURIN gene.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    FURIN (FURIN Human)
    Use
    Gateway entry vector
    Available Since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158442-plasmid-map-sequence-id-308251

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  43. QQR1 variant

    Plasmid
    #138564
    Purpose
    Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    QQR1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138564-plasmid-map-sequence-id-274793

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  46. pSNAP-CaaX Control Plasmid

    Plasmid
    #101133
    Purpose
    Expression of SNAP-Tag and CaaX box (Farnesylation Signal) in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Insert
    CaaX Box
    Tags
    SNAP-tag (SNAP26m)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101133-plasmid-map-sequence-id-194147

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  47. pCMV-ABE8e-eVRQR-P2A-EGFP (HES1425)

    Plasmid
    #242657
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with neVRQR(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8e-SpCas9-VRQR(S55R)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA, eVRQR mutations in SpCas…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242657-plasmid-map-sequence-id-488990

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  48. pAS_4xMma PylT_FLAG-PcKRS

    Plasmid
    #140022
    Purpose
    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    PcKRS (pylS Methanosarcina bakeri)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    M241F, A267S, Y271C, L274M
    Promoter
    EF1
    Available Since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140022-plasmid-map-sequence-id-297729

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  49. STING-V1

    Plasmid
    #124262
    Purpose
    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Tags
    Venus1
    Expression
    Mammalian
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124262-plasmid-map-sequence-id-241476

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  50. pDual_dsCas9_Blast

    Plasmid
    #214681
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214681-plasmid-map-sequence-id-428811

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Showing: 11701 - 11750 of 169450 results