Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

179,553 results

Showing: 11701 - 11750 of 179553 results

  6. pHN781

    Plasmid
    #178828
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Tags
    His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178828-plasmid-map-sequence-id-351929

    Enlarge

  7. pAAV-hSyn1-eYFP (AAV Retrograde)

    Viral prep
    #117382-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Publication
    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:
    Promoter
    Syn
    Tags
    eYFP
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  8. VPS13D^EGFP

    Plasmid
    #174109
    Purpose
    The insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.
    Depositor
    Pietro De Camilli
    Publication
    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized VPS13D
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    With an internal EGFP tag in aminoacid position 1…
    Promoter
    CMV
    Available since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    174109-plasmid-map-sequence-id-345250

    Enlarge

  10. H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

    Plasmid
    #129631
    Purpose
    Visualization of Akt and ERK activity
    Depositor
    Dorus Gadella
    Publication
    Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    129631-plasmid-map-sequence-id-260221

    Enlarge

  12. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid
    #170977
    Purpose
    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.
    Depositor
    Miki Ebisuya
    Publication
    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CShroom3 (Shroom3 Mouse)
    Use
    Gateway Cloning
    Tags
    SspB and mCherry
    Mutation
    Isoform X2, deleted aminoacids 1 - 1388
    Available since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170977-plasmid-map-sequence-id-336791

    Enlarge

  17. H2B-mGreenLantern

    Plasmid
    #164464
    Purpose
    Localization of mGreenLantern fluorescent protein to histone 2B
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mGreenLantern
    Tags
    Human Histone 2B (D26G and V119I mutations)
    Expression
    Mammalian
    Mutation
    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…
    Promoter
    CMV
    Available since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    164464-plasmid-map-sequence-id-322386

    Enlarge

  19. pAAV-nEF-Con/Foff 2.0-iC++-EYFP (AAV8)

    Viral prep
    #137156-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-iC++-EYFP (#137156). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-iC++-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of chloride-conducting channelrhodopsin iC++-EYFP (inhibited in presence of Flp) for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    EYFP (Cre-dependent)
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only

  25. Sc++

    Plasmid
    #155011
    Purpose
    Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells
    Depositor
    Joseph Jacobson
    Publication
    Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sc++
    Expression
    Mammalian
    Mutation
    T1227K, Loop Substitution from S. anginosus
    Promoter
    EF1ɑ
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    155011-plasmid-map-sequence-id-308829

    Enlarge

  30. pcDNA3.1-GGamma9-GFP2

    Plasmid
    #140991
    Purpose
    Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGamma9-GFP2 (GNGT2 Human)
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    140991-plasmid-map-sequence-id-521071

    Enlarge

  32. pGBW-m4137384

    Plasmid
    #149543
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike) for VSV pseudotyping
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Human)
    Expression
    Mammalian
    Mutation
    H. sapiens recode
    Promoter
    CMV
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    149543-plasmid-map-sequence-id-306267

    Enlarge

  33. pGBW-m4046828

    Plasmid
    #145742
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    145742-plasmid-map-sequence-id-298346

    Enlarge

  35. pBluescript-PB

    Plasmid
    #133885
    Purpose
    piggyBac ITR's in high copy number pBluescript backbone plasmid
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Piggybac
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    133885-plasmid-map-sequence-id-274419

    Enlarge

  36. CIDAR MoClo Extension, Volume I

    Plasmid kit
    #1000000161
    Purpose
    Plasmids to efficiently build a wide variety of gene expression constructs. Can be used as a stand-alone toolkit or with the CIDAR MoClo Parts Kit (Addgene #1000000059).
    Depositor
    Richard Murray
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only

  41. TOM20*-SBP-GFP

    Plasmid
    #120173
    Purpose
    Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)
    Depositor
    Juan Bonifacino
    Publication
    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)
    Tags
    SBP and eGFP
    Expression
    Mammalian
    Mutation
    Truncated TOM20: 1-30 aa
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    120173-plasmid-map-sequence-id-247521

    Enlarge

  43. pGP-AAV-syn-jGCaMP7c variant 1513-WPRE (AAV1)

    Viral prep
    #105321-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP7c variant 1513-WPRE (#105321). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7c variant 1513-WPRE plasmid DNA. Synapsin-driven jGCaMP7c expression. jGCaMP7c exhibits high contrast between peak fluorescence and resting fluorescence. It is useful for activity imaging of large populations of densely-labeled neurons because background fluorescence from inactive neurons is reduced. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only

  48. SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

    Plasmid
    #123615
    Purpose
    CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3(R33A/K34A)-P2A-EGFP
    Expression
    Mammalian
    Mutation
    R33A/K34A in rAPOBEC1, D10A in SpCas9
    Promoter
    CAG
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    123615-plasmid-map-sequence-id-239343

    Enlarge

Showing: 11701 - 11750 of 179553 results