We narrowed to 6,120 results for: nls
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Plasmid#103827PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorUseTagsExpressionMammalianMutationhGCN5: A566G (E189G), G585T (K195N), G726A (silen…PromoterCMVAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only
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DsRed-CPI-17-T38A-NLS
Plasmid#100792PurposeMammalian expression of Fluorescent CPI-17 neutral mutation nuclearDepositorInsertprotein phosphatase 1 regulatory inhibitor subunit 14A (PPP1R14A Human)
UseTagsDsRedExpressionMammalianMutationT38APromoterCMVAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
DsRed-CPI-17-T38E-NLS
Plasmid#100790PurposeMammalian expression of Fluorescent CPI-17 phospho mimetic nuclearDepositorInsertCPI-17-T38E-NLS (PPP1R14A Human)
UseTagsDsRedExpressionMammalianMutationT38EPromoterCMVAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2 3XFLAG-EGFP-NLS
Plasmid#87778PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK-HygroDepositorInserteGFP
UseTags3xFLAG and NLSExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2 3XFLAG-MKK7-EE-NLS
Plasmid#87777PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK-HygroDepositorInsertMKK7-EE
UseTags3xFLAG and NLSExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS
Plasmid#87776PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK-HygroDepositorInsertMKK4 (MAP2K4 Human)
UseTags3xFLAG and NLSExpressionMutationPromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-mCE NLS
Plasmid#82465PurposeExpresses myc tag and mRNA capping enzyme without Nuclear Localization Sequence (NLS) from C terminusDepositorInsertCapping Enzyme (Rngtt Mouse)
UseTagsMycExpressionMammalianMutationdeleted amino acids Nuclear Localization SignalPromoterCMVAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-3xFLAG-NLS-TPP1-OB
Plasmid#79060PurposeRetroviral expression of 3xFLAG tagged, nuclear localized wild-type TPP1-OB folding domainDepositorInsertTPP1 (TPP1 Human)
UseRetroviralTags3xFLAG and NLSExpressionMammalianMutationWild-type TPP-OB folding domainPromoterCMVAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1L-dC-NLS
Plasmid#51840PurposeFlag-CHES1L mutant lacking the C-terminal, with remaining NLSDepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsFlag and NLSExpressionMutationcontains CHES1 amino acids 1-205PromoterAvailable sinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1LdFC-NLS
Plasmid#51838PurposeFlag-CHES1L mutant lacking the forkhead binding site and C-terminal domain, with remaining NLSDepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsFlag and NLSExpressionMutationcontains CHES1 amino acids 1-109PromoterAvailable sinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)
Plasmid#196703PurposePlasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.DepositorInserthuman RNaseH1 D210N
UseTagsExpressionMammalianMutationD210N, catalytically inactivePromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-miniTurbo-YFP-NLS-STOP-L2
Plasmid#127362Purposegateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choiceDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, NLS, V5, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIG-V5-Snail2-IRES-nls-GFP
Plasmid#44282DepositorInsertV5-Snail2 (SNAI2 Chicken)
UseTagsV5ExpressionMammalianMutationPromoterCMV-IE-enhancer and chicken beta-actin promoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR
Plasmid#154003PurposeVector expressed CasRx and sgRNA_LACZ for AAV packageDepositorInsertCasRx
UseAAV and CRISPRTagsExpressionMutationPromoterEFSAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
phage UBC NLS-HA-2XMCP-tagRFPt
Plasmid#64541PurposeUsed to label reporter mRNAs. Lentiviral expression of the MCP protein fused to tagRFP.DepositorInsertMCP
UseLentiviralTagsHA, NLS, and tagRFP-TExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
phage ubc nls ha pcp gfp
Plasmid#64539PurposeUsed to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.DepositorInsertPCP
UseLentiviralTagsEGFP, FactorXa site, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBGreen-FIRE-puro
Plasmid#139195PurposeLuminescent reporter of ERK activityDepositorInsertCBG99
UseLuciferase and RetroviralTagsFra1 PEST domain fragment (FIRE) and nuclear loca…ExpressionMammalianMutationSee depositor comments belowPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
PET-B-Sniper SpCas9-NLS-6xHis
Plasmid#207377PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-Sniper SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationF539S, M763I, K890N, amino acids 1005-1013 replac…PromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-Sniper SpCas9-NLS-6xHis
Plasmid#207374PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertSniper SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationF539S, M763I, K890NPromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311/NLS-Cas13d-P2A-Blast
Plasmid#212196PurposeCasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40DepositorInsertCasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
UseCRISPR and LentiviralTagsHA, NLS, and blasticidin S deaminaseExpressionMammalianMutationPromoterEF-1α promoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-zeo_3xHA-TurboID-NLS
Plasmid#200022PurposeFor lentiviral transduction of 3xHA-TurboID-NLS under control of a doxycyline-inducible promoterDepositorInsert3xHA-TurboID-NLS
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GsAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
3XFLAG-VP64-SadCas9-NLS-VP64
Plasmid#135338Purpose3xFLAG 2xVP64 SadCas9 for single guide cloningDepositorInsertSadCas9
UseTags3xFLAG-VP64-SV40 NLS and NLS-VP64ExpressionMammalianMutationD10A and N580APromoterCMVAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVf-EnhCB-lacZnls miR-122 3x
Plasmid#35644DepositorInsert3 miR-122 target sites
UseAAVTagsExpressionMutationPromoterCBAvailable sinceApril 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-EBFP2-NLS (pRW1447)
Plasmid#216219PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAY5 (mAID-EGFP-NLS piggyBac)
Plasmid#140532PurposemAID-EGFP-NLS piggyBacDepositorInsertmAID-EGFP-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS
Plasmid#71396Purpose2Gi2R assayDepositorInserts2xGFP:NES
2xRFP:NLS
UseLentiviralTagsIRESExpressionMammalianMutationPromoterEF1alpha and IRESAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP
Plasmid#86827PurposeExpresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAsDepositorInsertFLAG-NLS-MS2-eGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-6xNLS-E174R/S542R (pRG232)
Plasmid#149723PurposeLentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG
Plasmid#225245PurposeMammalian expression of iPASnFR-H in the nucleusDepositorInsertiPASnFR-H
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
P2A-NLS-tdtomato-EPG backbone
Plasmid#215548PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFPDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUBC-SV40NLS-Pfv-Sapphire-PURO
Plasmid#203654PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.DepositorArticleInsertPfV
UseLentiviralTagsSapphireExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-2xflox-BRX-eGFP-NLS
Plasmid#217535PurposeAAV vector to drive the expression of eGFP in genetically defined cell populations under the control of the 4xBRE regulatory element of SMAD1 transcription factor and miniXon splicing casette in vivoDepositorArticleInsert4X BRE reporter and miniXon casette (Smad1 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterBMP response element (BRE)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NLS-iPASnFR-L-mScarlet3-3xFLAG
Plasmid#225246PurposeMammalian expression of iPASnFR-L in the nucleusDepositorInsertiPASnFR-L
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
NLS-Laconic/pCMV-myc-nuc
Plasmid#46307Purposea nuclear-targeted, genetically-encoded sensor for lactateDepositorInsertNLS-Laconic
UseLactate biosensorTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28-SpRY-NLS-6xHis (gbr2101)
Plasmid#181743Purposebacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tagDepositorInsertbacterial codon optimized SpRY
UseIn vitro transcription; t7 promoterTagsBPNLS(SV40)-6xHis and MKIEE MBP tagExpressionBacterialMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterT7Available sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
SBE-NLS-mCherry PGK-rtTA3
Plasmid#195332PurposeLentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3DepositorInsertsNLS-mCherry
rtTA3
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhPGK and miniCMVAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-3xSNAP-NLS
Plasmid#236388PurposeOverexpression of PCP-3xSNAP in human cellsDepositorInsertPCP-3xSNAP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC NLS HA stdMCP stdHalo
Plasmid#104999PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labelingDepositorInsertstdMCP-stdHalo
UseLentiviralTagsHA tag, NLS, Two synonymous copies of the Halo-ta…ExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
phage-ubc-nls-ha-tdPCP-gfp
Plasmid#40650DepositorInserttdPCP-gfp
UseLentiviralTagsEGFP, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C (UBC)Available sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
His-CL7-2NLS-SpyCas9(dS)-A22p3star
Plasmid#222970PurposeBacterial expression of A22p-fused SpyCas9 constructDepositorInsertHis-CL7-2NLS-SpyCas9(dS)-A22p3
UseCRISPRTagsCL7 and HisExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP
Plasmid#31484DepositorInsertEF1s HA NLS Sce(opt) T2A IFP1.4
UseLentiviralTagsHA and NLSExpressionMutation"Opt" indicates the I-SceI coding seque…PromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV 4xSTAR-mNeonGreen-NLS-blast
Plasmid#136258PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 4 repeats
UseLentiviralTagsmNeonGreen-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-NLS-copGFP-EF1-BlastiS
Plasmid#132772PurposeSpills into the cytoplasm during nuclear envelope rupture.DepositorInsertNLS-copGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-DEVD-mCardinal-NLS
Plasmid#164052PurposeFluorescent reporter for caspase-3DepositorInsertNES-mCardinal-NLS
UseTagskozak sequenceExpressionMammalianMutationPromoterCMV promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
phage-ubc-nls-ha-tdMCP-gfp
Plasmid#40649DepositorInserttdMCP-gfp
UseLentiviralTagsEGFP, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C (UBC)Available sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS
Plasmid#199252PurposeExpression construct encoding the high specificity eSpCas9(1.1)-D10A nickaseDepositorInsertHigh specificity eSpCas9(1.1)-D10A nickase
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10A K848A K1003A R1060APromoterCAG promoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-PyronicSF/pCMV-myc-nuc
Plasmid#124814PurposeHighly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.DepositorInsertPyronicSF
UseTagsNuclear Localization SignalExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SV40NLSf-mScarletP2A-hLuc
Plasmid#218793PurposeAAV reporter genome plasmidDepositorInsertNLSf-mScarletP2A-hLuc
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV Marina-T2A-nls-mCherry
Plasmid#74216Purposegenetically-encoded fluorescent voltage indicatorDepositorInsertfluorescent protein voltage sensor
UseTagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PET-HiFi SpCas9-NLS-6xHis
Plasmid#207376PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHiFi SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR691APromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only