299,785 results

1. pUC18T-mini-Tn7T-Gm-ecfp

   Plasmid

   #65030

   Purpose

   GmR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   ecfp
   

   Expression

   Bacterial

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC18T-mini-Tn7T-Gm-ecfp

   Plasmid

   #65030

   Purpose

   GmR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   ecfp
   

   Expression

   Bacterial

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GFP-ROCK1

   Plasmid

   #187296

   Purpose

   Express GFP ROCK1

   Depositor

   Alpha Yap

   Article

   Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

   Insert

   Rock1 (a.k.a. 1110055K06Rik, Rock-I) (Rock1 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP-ROCK1

   Plasmid

   #187296

   Purpose

   Express GFP ROCK1

   Depositor

   Alpha Yap

   Article

   Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

   Insert

   Rock1 (a.k.a. 1110055K06Rik, Rock-I) (Rock1 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TFORF1842

   Plasmid

   #143324

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   YWHAZ (YWHAZ Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TFORF1842

   Plasmid

   #143324

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   YWHAZ (YWHAZ Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR223-TLK2

   Plasmid

   #23629

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   TLK2 (TLK2 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pDONR223-TLK2

   Plasmid

   #23629

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   TLK2 (TLK2 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pET31b-T7-Spinach2-Ct

   Plasmid

   #79158

   Purpose

   Expresses the Ct biosensor under a T7 promoter

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Insert

   T7-tRNA-Spinach2-Ct
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET31b-T7-Spinach2-Ct

    Plasmid

    #79158

    Purpose

    Expresses the Ct biosensor under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    T7-tRNA-Spinach2-Ct
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pN1/G3BP1-iRFP

    Plasmid

    #129339

    Purpose

    Stress granule marker

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    iRFP (from Dr. M. Matsuda)
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pN1/G3BP1-iRFP

    Plasmid

    #129339

    Purpose

    Stress granule marker

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    iRFP (from Dr. M. Matsuda)
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV1)

    Viral Prep

    #26969-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

    Promoter

    CaMKII

    Tags

    EYFP

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

14. CpxR-GFP

    Plasmid

    #220150

    Purpose

    To track the natural CpxR promoter's transcriptional dynamics

    Depositor

    Andre Sanches Ribeiro

    Article

    Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

    Insert

    CpxR promoter only
    

    Expression

    Bacterial

    Available Since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lentiCRISPR v2-Nr4a1 double gRNAs

    Plasmid

    #162791

    Purpose

    Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Promoter

    hU6

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lentiCRISPR v2-Nr4a1 double gRNAs

    Plasmid

    #162791

    Purpose

    Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Promoter

    hU6

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SEAP (Protein secretion reporter) in pLX317

    Plasmid

    #233290

    Purpose

    SEAP reporter (used for monitoring protein secretion)

    Depositor

    Sefi Rosenbluh

    Article

    Xi et al Proc Natl Acad Sci U S A. 2025 Apr 15;12

    Insert

    SEAP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3.1(+)-NEMOb

    Plasmid

    #189934

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOb
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3.1(+)-NEMOb

    Plasmid

    #189934

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOb
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKm

    Plasmid

    #170331

    Purpose

    backbone vector for clone construction

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKm

    Plasmid

    #170331

    Purpose

    backbone vector for clone construction

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PX459v3

    Plasmid

    #178799

    Purpose

    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PX459v3

    Plasmid

    #178799

    Purpose

    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mEmerald-Talin-C-18

    Plasmid

    #62763

    Purpose

    Localization: Talin/Focal Adhesions, C1 Cloning Vector, Excitation:487, Emission:509

    Depositor

    Michael Davidson

    Insert

    Talin
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. mEmerald-Talin-C-18

    Plasmid

    #62763

    Purpose

    Localization: Talin/Focal Adhesions, C1 Cloning Vector, Excitation:487, Emission:509

    Depositor

    Michael Davidson

    Insert

    Talin
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. FUGW-APPIce-T2A-mCherry

    Plasmid

    #190806

    Purpose

    Express Icelandic mutant APP from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Insert

    APP (APP Human)
    

    Use

    Lentiviral

    Mutation

    Icelandic (A673T)

    Promoter

    Ubi C

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FUGW-APPIce-T2A-mCherry

    Plasmid

    #190806

    Purpose

    Express Icelandic mutant APP from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Insert

    APP (APP Human)
    

    Use

    Lentiviral

    Mutation

    Icelandic (A673T)

    Promoter

    Ubi C

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 EYFP H148Q/I152L

    Plasmid

    #25872

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q/I152L
    

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with H148Q and I152L m…

    Available Since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1 EYFP H148Q/I152L

    Plasmid

    #25872

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q/I152L
    

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with H148Q and I152L m…

    Available Since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBABEpuro-ERBB2 A775_G776insV, G776C

    Plasmid

    #40979

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    A775_G776insV, G776C

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pBABEpuro-ERBB2 A775_G776insV, G776C

    Plasmid

    #40979

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    A775_G776insV, G776C

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

32. pAS_4xMma PylT sfGPF 150TAG

    Plasmid

    #154766

    Purpose

    plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG in GFP reporter, U25C in PylT

    Promoter

    EF1

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAS_4xMma PylT sfGPF 150TAG

    Plasmid

    #154766

    Purpose

    plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG in GFP reporter, U25C in PylT

    Promoter

    EF1

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET29b_1_anti-GFP-nanobody

    Plasmid

    #210394

    Purpose

    Express anti-GFP nanobody in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    1_anti-GFP-nanobody
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pEGFP-C3-ZO-2

    Plasmid

    #27422

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Available Since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pEGFP-C3-ZO-2

    Plasmid

    #27422

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Available Since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

37. pcDNA3.1-p300

    Plasmid

    #23252

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    p300 (EP300 Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1-p300

    Plasmid

    #23252

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    p300 (EP300 Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFBOH-MHL_EIF3B:127-814

    Plasmid

    #210909

    Purpose

    Insect expression of human EIF3B fragment D127 to E814. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    EIF3B:D127-E814 (EIF3B Human)
    

    Tags

    MHHHHHHSSGRENLYFQGMQ

    Expression

    Insect

    Mutation

    Y760C sequence conflict

    Promoter

    Polyhedrin

    Available Since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. FUGW-3xHA-KCNJ6-mCherry

    Plasmid

    #197032

    Purpose

    Express 3xHA-tagged human KCNJ6 (Girk2), co-expressed with mCherry, from UbiC promoter

    Depositor

    Ronald Hart

    Insert

    KCNJ6 (KCNJ6 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge