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164,909 results

Showing: 10601 - 10640 of 164909 results

  4. TRUPATH Triple GaoB

    Plasmid
    #196052
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196052-plasmid-map-sequence-id-387470

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  5. Talin-TS

    Plasmid
    #83376
    Purpose
    FRET probe for measuring tension on Talin molecule
    Depositor
    Martin Schwartz
    Article
    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d
    Insert
    Talin1 (Tln1 Mouse)
    Use
    Retroviral
    Tags
    EGFP-F40-tagRFP (tension sensor module)
    Expression
    Mammalian
    Mutation
    Tension sensor module is inserted between V447 an…
    Promoter
    CMV
    Available since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    83376-plasmid-map-sequence-id-158760

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  7. PG1424PGA

    Plasmid
    #74652
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PG1424PGA
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. pET_RTX(exo-)-6xHis

    Plasmid
    #145028
    Purpose
    pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag
    Depositor
    Andrew Ellington
    Article
    Bhadra et al BioProtocol 2021
    Insert
    RTX(exo-)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    N210D
    Promoter
    T7
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    145028-plasmid-map-sequence-id-292638

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  19. pHnS 2comp KI;Empty

    Plasmid
    #240302
    Purpose
    Empty vector
    Depositor
    Yuki Ogawa
    Insert
    Empty gRNA
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    240302-plasmid-map-sequence-id-478528

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  20. pAAV 3xgRNA KO;Template

    Plasmid
    #240310
    Purpose
    KO:Template
    Depositor
    Yuki Ogawa
    Insert
    Template gRNA
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    Available since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240310-plasmid-map-sequence-id-478217

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  21. pNluc-hbb

    Plasmid
    #239753
    Purpose
    Template for in vitro transcription of nanoluciferase flanked by the human haemoglobin beta 5' and 3' UTRs.
    Depositor
    Catherine Jopling
    Article
    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3
    Insert
    Nanoluciferase flanked by human haemoglobin beta 5' and 3' UTRs
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    T7 (included as part of insert)
    Available since
    July 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    239753-plasmid-map-sequence-id-477838

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  22. 8522-E06

    Plasmid
    #234291
    Purpose
    Gateway ORF Entry clone of human Tgfbr1 with stop codon (for native or N-terminal fusions); T204D mutation
    Depositor
    Dominic Esposito
    Insert
    Tgfbr1 T204D (Tgfbr1 Mouse)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    T204D
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234291-plasmid-map-sequence-id-468906

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  23. pYPQAT42

    Plasmid
    #223414
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpRY-D10A-ZmUbi-gRNA scaffold-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223414-plasmid-map-sequence-id-454172

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  24. pYPQAT21

    Plasmid
    #223393
    Purpose
    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by 2x35s and the sgRNA was driven by AtU3 promoter.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223393-plasmid-map-sequence-id-453501

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  25. pYPQAT09

    Plasmid
    #223381
    Purpose
    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-SpRY-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223381-plasmid-map-sequence-id-453508

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  28. Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)

    Plasmid
    #207356
    Purpose
    Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.
    Depositor
    Marianne Carlon
    Article
    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d
    Insert
    N1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207356-plasmid-map-sequence-id-408417

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  30. B-Sniper SpCas9

    Plasmid
    #207361
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-Sniper SpCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207361-plasmid-map-sequence-id-410129

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  31. pTXBI-mClover-YTH domain

    Plasmid
    #177123
    Purpose
    Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Article
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTH domain) (YTHDC1 Human)
    Use
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    YTH domain only (IDR1 and IDR2 deletion)
    Promoter
    T7
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    177123-plasmid-map-sequence-id-348690

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  33. pAAV-hSyn-DO-ChroME2f-ST-P2A-GCaMP8s

    Plasmid
    #227786
    Purpose
    Cre off - Cation channelrhodopsin ChroME2f fused to GCaMP8m and targeted to the neuronal soma and proximal dendrites
    Depositor
    Hillel Adesnik
    Insert
    DO-ChroME2f-ST-P2A-GCaMP8s
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    227786-plasmid-map-sequence-id-458443

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  36. EF1a- gfp-2a-puro

    Plasmid
    #129443
    Purpose
    expression of GFP and Puro R
    Depositor
    Oskar Laur
    Insert
    gfp-puro
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129443-plasmid-map-sequence-id-256478

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  38. psiCHECK2-let-7 MT

    Plasmid
    #78261
    Purpose
    psiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene
    Depositor
    Joanne Weidhaas
    Article
    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10
    Insert
    mutated let-7 target site
    Use
    Luciferase; Microrna activity
    Tags
    Expression
    Mammalian
    Mutation
    nucleotides 2-5 and 10-11 changed from CTCC to GA…
    Promoter
    Available since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    78261-plasmid-map-sequence-id-149478

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Showing: 10601 - 10640 of 164909 results