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Addgene
Showing: 10601 - 10640 of 160936 results

  3. AYBEv3

    Plasmid
    #193967
    Purpose
    An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Insert
    ecTadA8e-nSpCas9(D10A)-MPG(v3)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G163R, N169S, S198A, K202A, G203A, S206A, K210A
    Promoter
    hU6, EF1a, CBh
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    193967-plasmid-map-sequence-id-381640

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  8. FUGW-VGLUT1-pHluorin

    Plasmid
    #207470
    Purpose
    Lentiviral expression of VGLUT1-pHluorin
    Depositor
    Robert Edwards
    Insert
    VGLUT1 (Slc17a7 Rat)
    Use
    Lentiviral
    Tags
    pHluorin
    Expression
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    207470-plasmid-map-sequence-id-410343

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  10. cytoplasmic NAD+ biosensor

    Plasmid
    #186787
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm
    Depositor
    Michael Cohen
    Article
    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d
    Insert
    cytoplasmic NAD+ biosensor
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186787-plasmid-map-sequence-id-368056

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  11. pLKO-Tet-On-ARNTL-shRNA2

    Plasmid
    #166914
    Purpose
    Lentivirus for inducible knockdown of human ARNTL(BMAL1)
    Depositor
    Roland Friedel
    Insert
    ARNTL shRNA (BMAL1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    166914-plasmid-map-sequence-id-331230

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  14. TFORF1497

    Plasmid
    #143126
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    LYL1 (LYL1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143126-plasmid-map-sequence-id-397859

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  15. pelBHisTEV

    Plasmid
    #157741
    Purpose
    empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Use
    Tags
    PelB signal sequence + His10-tag + TEV protease c…
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    157741-plasmid-map-sequence-id-334005

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  17. pORF1-Flag

    Plasmid
    #190565
    Purpose
    Mammalian expression plasmid encoding the first open reading frame (ORF1, expressing the protein ORF1p) of human L1 (L1.3); carries a C-terminus Flag tag.
    Depositor
    Anthony Furano
    Article
    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112
    Insert
    ORF1 (from L1.3)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    190565-plasmid-map-sequence-id-393221

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  21. pET28a-CohIII-CBM-HIS-ybbR

    Plasmid
    #60866
    Purpose
    Characteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tag
    Depositor
    Hermann Gaub
    Article
    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1
    Inserts
    CohIII
    CBM
    Use
    Tags
    6xHIS, HRV 3C, and ybbR
    Expression
    Bacterial
    Mutation
    Cysteine 63 to Serine in CBM module
    Promoter
    T7
    Available since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    60866-plasmid-map-sequence-id-98102

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  33. p667-UBC-CDK12-V5_IDG-K

    Plasmid
    #135245
    Purpose
    Gateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK12-V5 (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135245-plasmid-map-sequence-id-268084

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  34. TFORF1847

    Plasmid
    #142868
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ETS1 (ETS1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    142868-plasmid-map-sequence-id-385800

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  35. pBXNPHM3

    Plasmid
    #110099
    Purpose
    E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage site
    Depositor
    Markus Seeger
    Article
    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1
    Type
    Empty backbone
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110099-plasmid-map-sequence-id-258124

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Showing: 10601 - 10640 of 160936 results