167,318 results

1. pLJM1-Lyso-FLAG-GFP-Sac1-Cat-WT

   Plasmid

   #134645

   Purpose

   lysosome-targeted Sac1 catalytic domain wild type

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   Sac1 catalytic domain (77-520 aa) (SACM1L Human)
   

   Use

   Lentiviral

   Tags

   FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…

   Expression

   Mammalian

   Mutation

   contains only amino acids 77-520 (Please see depo…

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. plenti-ef1a-dCasRx-FTO-HA-T2A-BSD

   Plasmid

   #177120

   Purpose

   dCasRx mediated RNA tethering of FTO

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   dCasRx-FTO
   

   Use

   Lentiviral

   Tags

   HA and SV40 NLS

   Mutation

   dCasRX contains R239A/H244A/R858A/H863A mutations…

   Promoter

   Ef1a

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLZRS-IRES-deltaNGFR

   Plasmid

   #72930

   Purpose

   retroviral expression with FACS-selectable marker (extracellular part of NGFR)

   Depositor

   Esther van de Vosse

   Article

   van_de_Vosse et al Blood. 2009 May 7;113(19):4548-55. doi:

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-HIF2alpha-P405A/P531A-pBabe-puro

   Plasmid

   #19006

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: P405A and P531A

   Available Since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK465 (mAB-Hygro)

   Plasmid

   #214391

   Purpose

   mAID-BromoTag for C-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   mAID-BrpmpTag-Hygro
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-dLight1.1 (AAV5)

   Viral Prep

   #111067-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-CAG-dLight1.1 (#111067). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.1 plasmid DNA. CAG-driven expression of dLight1.1 dopamine sensor. This sensor is recommended for photometry in vivo. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Available Since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pAAV FLEX Synaptophysin GFP

   Plasmid

   #137188

   Purpose

   Cre-dependent expression of synaptophysin-GFP

   Depositor

   Matthew Nolan

   Article

   Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

   Insert

   Synaptophysin GFP (Syp Mouse)
   

   Use

   AAV

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIG-601_NY-ESO-1_TCR_GFP

   Plasmid

   #207482

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   GFP, NY-ESO-1_TCR
   

   Use

   CRISPR

   Mutation

   Potential silent point mutation in GFP sequence

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-HA-pcDNA3.1-

   Plasmid

   #52535

   Purpose

   enables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteins

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   FLAG-HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMG051 MBP-β-catenin-His, GST-CK1α

    Plasmid

    #154072

    Purpose

    Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Inserts

    CTNNB1 (CTNNB1 Human)
    CSNK1A1 (CSNK1A1 )
    

    Tags

    GST, His6, and MBP

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT525

    Plasmid

    #131392

    Purpose

    L1RP retrotransposition reporter

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    L1RP GFP-AI
    

    Expression

    Mammalian

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. shp53 pLKO.1 puro

    Plasmid

    #19119

    Purpose

    3rd gen lentiviral vector for knocking down p53 gene expression

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    small hairpin RNA against tumor protein p53 (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9_T2A_mCherry sg1-sg2

    Plasmid

    #192479

    Purpose

    All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP

    Plasmid

    #198863

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human TERT gene

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    TERT sgRNA (TERT Human)
    

    Expression

    Mammalian

    Promoter

    u6

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-hfYFP

    Plasmid

    #186523

    Purpose

    Hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Hyperfolder YFP
    

    Expression

    Mammalian

    Mutation

    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiGuideFE-Puro

    Plasmid

    #170069

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    Cas9 guide RNA scaffold with the F+E scaffold modification
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCXLE-Sox2AV-T2A-Klf4-E2A-Myc

    Plasmid

    #210017

    Purpose

    episomal SKM (expressing mouse Sox2-A61V + Klf4 + Myc) for integration-free naïve reset.

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Sox2AV-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
    

    Expression

    Mammalian

    Mutation

    Sox2A61V is a point mutant of Sox2 that enhances …

    Promoter

    CAG

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDUAL SRBI (GFP)

    Plasmid

    #86979

    Purpose

    Lentiviral expression construct encoding SR-B1 and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    SCARB1 (SCARB1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV/hPGK

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-GFP-Kir2.1

    Plasmid

    #107184

    Purpose

    mammalian expression of Kir2.1

    Depositor

    Bikash Pattnaik

    Insert

    KCNJ2 (KCNJ2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-HIF2α wt-pBabe-Puro

    Plasmid

    #26055

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMK486 (Hygro-P2A-2mAID)

    Plasmid

    #214398

    Purpose

    2mAID for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Hygro-P2A-2mAID
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pSiMPlk_N + pSiMPlk_C

    Plasmid

    #134310

    Purpose

    Maintenance of 2 plasmids with a single antibiotic in E. coli

    Depositor

    Barbara Di Ventura

    Article

    Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

    Insert

    kanR_gp41-1 N-intein + kanR_gp41-1 C-intein
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. mTagBFP2-PCNA-19-SV40NLS-4

    Plasmid

    #55320

    Purpose

    Localization: Nucleus, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    PCNA-19 SV40NLS (PCNA Human)
    

    Tags

    3xSV40 NLS and mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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24. pMK464 (mAB-Neo)

    Plasmid

    #214390

    Purpose

    mAID-BromoTag for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    mAID-BromoTag-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pMSCV40-EF1a-hBCL10-SO

    Plasmid

    #75256

    Purpose

    retroviral expression vector for expression of StrepOne-Tagged human BCL10

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    Bcl10 (BCL10 Human)
    

    Use

    Retroviral

    Tags

    StrepOne Tag

    Expression

    Mammalian

    Promoter

    pMSCV-LTRs, EF1a

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. TRE:GFP

    Plasmid

    #163036

    Purpose

    FLiCRE reporter gene for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    GFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pMK449 (BromoTag-mClover-BSD)

    Plasmid

    #214379

    Purpose

    BromoTag-mClover for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BromoTag-mClover-BSD
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. iRFP-H2A

    Plasmid

    #158691

    Purpose

    This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    iRFP670
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. Cys-3HB-SPYcatcher

    Plasmid

    #214838

    Purpose

    This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

    Insert

    Cys-3HB-SPYcatcher
    

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. U6-sgRNA-GFP

    Plasmid

    #200068

    Purpose

    For the knock down of GFP with an astrocyte specific mCherry expression

    Depositor

    Baljit Khakh

    Article

    Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

    Insert

    U6-sgRNA-GFP
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    GfaABC1D-mCherry

    Promoter

    U6, GfaABC1D

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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