165,637 results

1. pAAV-hSyn-dF-HA-KORD-IRES-mCitrine

   Plasmid

   #65417

   Purpose

   AAV packaging vector for Cre-dependent, hSyn driven KOR DREADD expression.

   Depositor

   Bryan Roth

   Article

   Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

   Has Service

   AAV8

   Insert

   hSyn-dF-HA-KORD-IRES-mCitrine (OPRK1 Human)
   

   Use

   AAV

   Tags

   HA and mCitrine

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRsetB-PercevalHR

   Plasmid

   #49081

   Purpose

   Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

   Depositor

   Gary Yellen

   Article

   Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

   Insert

   PercevalHR
   

   Use

   Tags

   7xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXS-IRES-Blast ACSL4

   Plasmid

   #173164

   Purpose

   Retroviral vector to express sgRNA resistant human ACSL4

   Depositor

   Kivanc Birsoy

   Article

   Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

   Insert

   Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Synonymous mutations at sgRNA sites

   Promoter

   MMLV

   Available since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMM534

   Plasmid

   #112530

   Purpose

   pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

   Depositor

   Timothy Lu

   Article

   Mimee et al Science. 2018 May 25;360(6391):915-918.

   Insert

   luxCDABE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PL(HrtO)

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PermaPhos Kit

   Plasmid Kit

   #1000000226

   Purpose

   This kit is used for expressing recombinant proteins with site-specific, non-hydrolyzable phosphoserine (nhpSer) in an engineered self-sufficient E. coli genetic code expansion (GCE) expression system

   Depositor

   Ryan Mehl

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

6. mTagBFP2-Annexin-12

   Plasmid

   #55276

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   Annexin (A4) (ANXA4 Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. TALED_Right-ND1-AD

   Plasmid

   #183900

   Purpose

   Expresses TALED_Right-ND1-AD in mammalian cells

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

   Insert

   TALED for ND1 mutation
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHIV-INK4A-zsGreen

   Plasmid

   #110728

   Purpose

   Mammalian Expression of p16INK4A IRES zsGreen

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   p16INK4A IRES zsGreen (CDKN2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF1230

   Plasmid

   #144092

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   AFF3 (AFF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-link-ymScarletI3-Kan

    Plasmid

    #224077

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selection

    Depositor

    Bas Teusink

    Insert

    ymScarletI3
    

    Use

    Tags

    ymScarletI3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pProEx Rac1(G12C) CL

    Plasmid

    #224269

    Purpose

    Protein purification of Rac1 (G12C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G12C

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SP1-SB100X

    Plasmid

    #154887

    Purpose

    Sleeping Beauty transposase fused to SP1

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

    Insert

    SP1-Sleeping Beauty
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD649-HAsp-JAG1-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #157024

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    JAG1 (JAG1 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. CNR1-DuET

    Plasmid

    #213213

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CNR1 (CNR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBa.Halo-LDLR

    Plasmid

    #162717

    Purpose

    Mammalian expression of human LDLR

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    LDLR (LDLR Human)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta actin

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

    Plasmid

    #135814

    Purpose

    Fluorescent reporter for knock-in of TdTomato into the TH locus

    Depositor

    Lee Rubin, Tim Ahfeldt

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
    

    Use

    Tags

    p2a-TdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAC_C_LTK

    Plasmid

    #187762

    Purpose

    MAC-tagged gene expression of human LTK

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    LTK (LTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TGT-His

    Plasmid

    #138201

    Purpose

    Expresses E. Coli TGT with a C-terminal His Tag

    Depositor

    Neal Devaraj

    Article

    Busby et al Methods Enzymol. 2020;641:373-399. doi:

    Insert

    E Coli tRNA Guanine Transglycosylase-His
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEThT-RvLEAM

    Plasmid

    #90033

    Purpose

    To express His-tagged RvLEAM in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

    Insert

    RvLEAM
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-RhoA

    Plasmid

    #23224

    Depositor

    Channing Der

    Article

    Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pDONR223-EGFR

    Plasmid

    #23935

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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22. hCRISPRa-v2, genome-wide, 10 sgRNAs/gene

    Pooled Library

    #1000000091

    Purpose

    Genome-wide CRISPR gRNA pooled library for activation of human genes

    Depositor

    Jonathan Weissman

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

23. VPR-ps-dSpCas9

    Plasmid

    #102855

    Purpose

    A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    dSpCas9, pdDronpa1.2
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS and VP64-p64-Rta transcription activator

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SP6-VEE-GFP

    Plasmid

    #58976

    Purpose

    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Insert

    EGFP
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    26S subgenomic promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLminP_Luc2P_RE25

    Plasmid

    #90367

    Purpose

    Notch - NIDC/CBF1 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Notch

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ELP-TEMP/pcDNA3.1(-)

    Plasmid

    #178434

    Purpose

    Genetically encoded ratiometric fluorescent thermometer

    Depositor

    Takeharu Nagai

    Article

    Vu et al Sci Rep. 2021 Aug 13;11(1):16519. doi: 1

    Insert

    ELP-TEMP
    

    Use

    Tags

    No tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBABE HAII

    Plasmid

    #14738

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-EML4-ALK variant 3a

    Plasmid

    #183829

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) in mammalian cells

    Depositor

    Aaron Hata

    Article

    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBiT3.1-N_SCAP:1-905

    Plasmid

    #211228

    Purpose

    Mammalian expression of full-length human SCAP isoform 2. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    SCAP:M1-D905 (SCAP Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    isoform 2. V798I natural variant (VAR_012203)

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TRUPATH Triple Ga15

    Plasmid

    #196060

    Purpose

    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET22-HT/plsA

    Plasmid

    #73625

    Purpose

    Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

    Depositor

    T. J. Kappock, James Williamson

    Article

    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

    Insert

    adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145

    Plasmid

    #111731

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q145 (polyQ repeat)

    Promoter

    CMV and P10

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCX-cMyc

    Plasmid

    #19772

    Purpose

    Non-integrating (episomal) expression of mouse c-Myc

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Science. 2008 Nov 7. 322(5903):949-53.

    Insert

    c-Myc (Myc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPM_Pveg-sfgfp

    Plasmid

    #121503

    Purpose

    Integration vector that expresses gfp in S. mutans

    Depositor

    Robert Shields

    Article

    Shields et al Appl Environ Microbiol. 2019 May 17. pii

    Insert

    Superfolder GFP
    

    Use

    S. mutans integration vector, integrates at the m…

    Tags

    Expression

    Mutation

    Promoter

    Pveg

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. wt dynamin 2 pEGFP

    Plasmid

    #34686

    Depositor

    Sandra Schmid

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D406G, V664A, H692R and Q758R

    Promoter

    CMV

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MiniCoopR 2xU6:gRNA, mitfa:Cas9

    Plasmid

    #118844

    Purpose

    Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Type

    Empty backbone

    Use

    CRISPR; Tol2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pKD4_mCherry

    Plasmid

    #187386

    Purpose

    To chromosomally tag genes of interest with mCherry. FRT-mCherry-kan-FRT

    Depositor

    Roi Avraham

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCRISPaint-TagBFP-PuroR

    Plasmid

    #80969

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    TagBFP
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CMV-ER-LAR-GECO1

    Plasmid

    #61244

    Purpose

    Expresses LAR-GECO1 in the endoplasmic reticulum in mammalian cells

    Depositor

    Robert Campbell

    Article

    Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

    Insert

    LAR-GECO1
    

    Use

    Tags

    ER-retention sequence: KDEL and ER-targeting sequ…

    Expression

    Mammalian

    Mutation

    Substitutions relative to R-GECO1: V51W/I113V/N35…

    Promoter

    CMV

    Available since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR

    Plasmid

    #157744

    Purpose

    knock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expression

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    chlorotoxin containing chimeric antigen receptor targeting glioblastoma
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge