167,910 results

1. pT22-GluCNZ-6His

   Plasmid

   #213504

   Purpose

   Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.

   Depositor

   John Denu

   Article

   Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12

   Insert

   Glucanase (glucan endo-1,3-beta-glucosidase)
   

   Tags

   6His tag

   Expression

   Bacterial

   Mutation

   1-36 Signal Sequence Deletion

   Promoter

   T7

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. His6_MBP_FMRP_P451S

   Plasmid

   #198693

   Purpose

   Expression of human FMRP protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FMR1 (FMR1 Human)
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed proline 451 to serine

   Promoter

   T7

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. hCathepsin S

   Plasmid

   #11251

   Purpose

   Mammalian expression of human Cathepsin S

   Depositor

   Hyeryun Choe

   Article

   Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

   Insert

   cathepsin S (CTSS Human)
   

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pEGFP×2-N1

   Plasmid

   #86775

   Purpose

   A mammalian expression vector with double EGFP at C-terminus.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3 Flag Jnk1a1

   Plasmid

   #13798

   Depositor

   Roger Davis

   Article

   Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

   Insert

   Jnk1a1 (MAPK8 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCVpuro-Venus

   Plasmid

   #96940

   Purpose

   Retroviral plasmid containing Venus for labeling of cells

   Depositor

   Lee Grimes

   Article

   Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

   Insert

   Venus Fluorescent Protein
   

   Expression

   Mammalian

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3xFLAG SARS-CoV2 RdRp.co

   Plasmid

   #154008

   Purpose

   Expression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cells

   Depositor

   Patrick Moore

   Article

   Meng et al J Med Virol. 2023 Jan;95(1):e28246. doi:

   Insert

   Codon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)
   

   Tags

   3xFLAG tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pDONR223_TMPO_WT

   Plasmid

   #81818

   Purpose

   Gateway Donor vector containing TMPO , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   TMPO (TMPO Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-TadA8e-BLM

   Plasmid

   #221226

   Purpose

   Express HACE Editor as a fusion of TadA-8e adenosine deaminase and BLM helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   TadA8e-BLM
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL-p21UTR

    Plasmid

    #20014

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR (Cdkn1a Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pVITRO-HPV33 L1L2

    Plasmid

    #52493

    Purpose

    Expresses HPV33 L1 and L2 in mammalian cells

    Depositor

    Richard Roden

    Article

    Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

    Inserts

    HPV33 L1
    HPV33 L2
    

    Expression

    Mammalian

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. PET-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207376

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459-sgAAVS1

    Plasmid

    #184403

    Purpose

    pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    AAVS1-specific sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. mNeonGreen-mRuby2-FRET-10

    Plasmid

    #58179

    Purpose

    Localization: Cytosolic FRET, Excitation: , Emission:

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2013 May;10(5):407-9. doi:

    Type

    Empty backbone

    Tags

    mNeonGreen and mRuby2

    Expression

    Mammalian

    Available Since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.CAG.iGluSnFR3.v857.PDGFR

    Plasmid

    #178333

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. H2B-mNeonGreen-IRESpuro2

    Plasmid

    #183745

    Purpose

    Expresses H2B-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    H2B (H2BC16P Human)
    

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOSIP-KL-mcherry

    Plasmid

    #116947

    Purpose

    Integrating mCherry into lambda attB in E. coli chromosome.

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Insert

    mcherry
    

    Available Since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAR028 Lenti pEF-dEnAsCas12a-NLS-ZNF705F KRAB-3XHA-T2A-BSD

    Plasmid

    #195545

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-TopKRAB-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX307-VHL(C162F)-3F-BirA

    Plasmid

    #220149

    Purpose

    Expresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    von Hippel-Lindau tumor suppressor (VHL Human)
    

    Use

    Lentiviral

    Tags

    3F-BirA

    Expression

    Mammalian

    Mutation

    changed cysteine 162 to phenylalanine; codon opti…

    Promoter

    EF-1a

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-EGFP (AAV5)

    Viral Prep

    #50465-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    EGFP

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pJYScr

    Plasmid

    #132719

    Purpose

    Plasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistance

    Depositor

    Jan Marienhagen

    Article

    Krumbach et al ACS Synth Biol. 2019 Dec 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3.1-MS2-HA-HsRoquin1_AE

    Plasmid

    #148611

    Purpose

    Mammalian Expression of HsRoquin1

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin1 (RC3H1 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. SNAP-Rab5

    Plasmid

    #169069

    Purpose

    Express human Rab5b in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rab5B (RAB5B Human)
    

    Tags

    SNAP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pT7-7 asyn E46K

    Plasmid

    #105728

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

    Insert

    alpha-synuclein E46K (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    E46K

    Promoter

    T7

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pet21_TurboID_18A

    Plasmid

    #170922

    Purpose

    Express TurboID_18A in Bl21

    Depositor

    Huan Bao

    Article

    Bao et al Commun Biol. 2021 Apr 30;4(1):514. doi:

    Insert

    TurboID-18a
    

    Expression

    Bacterial

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pTRE3G_SS-HA-V5-LaccID-CD4 TM_Puro

    Plasmid

    #234047

    Purpose

    expresses LaccID on the mammalian cell surface, dox-inducible lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

    Insert

    LaccID-CD4TM
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. LC-E18

    Bacterial Strain

    #115924

    Purpose

    Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Bacterial Resistance

    None

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

28. pCMV-monSTIM1

    Plasmid

    #161795

    Purpose

    Mammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)

    Depositor

    Won Do Heo

    Article

    Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

    Insert

    MonSTIM1 (STIM1 Human, Mustard Weed)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AICSDP-49: MYL2-mEGFP

    Plasmid

    #114414

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pBK1340-LV-DUAL-dGFP-NanoLuc-Puro

    Plasmid

    #223160

    Purpose

    Expression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-life

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    dGFP-P2A-NanoLuc
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBad-mCherry-XL (R125K)

    Plasmid

    #188054

    Purpose

    Expresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteria

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

    Insert

    mCherry-XL (R125K)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    differs from mCherry by R125K, W143S, I161V, Q163…

    Promoter

    araBAD

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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32. DL017:CMV::HaloTag-iPAK4

    Plasmid

    #177882

    Purpose

    HT-iPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDNA3 Flag Jnk3a2

    Plasmid

    #13759

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk3a2 (MAPK10 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

34. AiP14478 - pAAV-AiE0456m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4478)

    Plasmid

    #229923

    Purpose

    AiE0456m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

    Depositor

    Jonathan Ting, Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

    Plasmid

    #100941

    Purpose

    Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Human, Synthetic)
    

    Tags

    1xHA and 3xTy1

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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36. LLP339 pGK-dCas9-SunTag

    Plasmid

    #100943

    Purpose

    dCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1a

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    dCas9, SunTag array (10xGCN4)
    

    Use

    CRISPR

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMZ374

    Plasmid

    #59896

    Purpose

    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDNA3 Flag Jnk2a1

    Plasmid

    #13754

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk2a1 (MAPK9 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

39. pKL038 Lenti U6 dCas12a gRNA Puro mCherry

    Plasmid

    #195546

    Purpose

    dCas12a gRNA expression backbone

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti U6- empty cassette_Direct repeat_puro_mcherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLifeAct-miRFP703

    Plasmid

    #79993

    Purpose

    F-Actin labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    Lifeact
    

    Tags

    miRFP703

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge