167,913 results

1. eScaf

   Bacterial Strain

   #220921

   Purpose

   cssDNA production strain

   Depositor

   Shawn Douglas

   Article

   Shen et al Nucleic Acids Res. 2024 Apr 24;52(7):409

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

2. pRN3P_Kdm5b_MUT

   Plasmid

   #119767

   Purpose

   in vitro transcription for mRNA

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Borsos et al Nature. 2019 May;569(7758):729-733. doi:

   Insert

   Kdm5b MUT
   

   Available Since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. ZsGreen1-N1

   Plasmid

   #54702

   Purpose

   Localization: N1 Cloning Vector, Excitation: 493, Emission: 505

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   ZsGreen1

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV5)

   Viral Prep

   #162381-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (#162381). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   CAG

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

5. pHR_Gal4UAS_tBFP_PGK_mCherry

   Plasmid

   #79130

   Purpose

   Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Insert

   tBFP
   

   Use

   Lentiviral

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEGFP

   Plasmid

   #18696

   Depositor

   Karel Svoboda

   Article

   Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

   Insert

   monomeric EGFP
   

   Expression

   Mammalian

   Mutation

   A206K mutation

   Available Since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. attP-puro donor

   Plasmid

   #182139

   Purpose

   attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   attP-EGFP-EF1α-PuroR-T2A-TagBFP
   

   Expression

   Mammalian

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-CMV-GCaMP6s

   Plasmid

   #40753

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Insert

   GCaMP6s
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-HAPLN1

   Plasmid

   #200777

   Purpose

   Expresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter

   Depositor

   Alexander Dityatev

   Article

   Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

   Insert

   Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
   

   Use

   AAV

   Tags

   cfGFP

   Promoter

   Synapsin

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUBC-SV40NLS-Pfv-Sapphire

    Plasmid

    #203653

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSFFV_sfCherry2(1-10)

    Plasmid

    #82603

    Purpose

    Lentiviral vector for constitutive expression of sfCherry2(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(1-10)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    changed Glu 118 to Gln, changed Thr 128 to Ile

    Promoter

    SFFV

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV_3xFLAG-ATP6V0A3-mScarlet

    Plasmid

    #228867

    Purpose

    Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V0A3 (TCIRG1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG and mScarlet

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG426GPD-ccdB

    Plasmid

    #14156

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Expression

    Yeast

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. L13-Arl13bGFP

    Plasmid

    #40879

    Purpose

    3rd generation transfer vector

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (Pax6)

    Plasmid

    #188383

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Pax6(DBD)-p65(428-551) transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Pax6(DBD)-p65(428-551)
    

    Use

    Lentiviral

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACAGW-ChR2-Venus-AAV (AAV1)

    Viral Prep

    #20071-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pACAGW-ChR2-Venus-AAV (#20071). In addition to the viral particles, you will also receive purified pACAGW-ChR2-Venus-AAV plasmid DNA. CAG-driven, channelrhodopsin fused to Venus for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karel Svoboda

    Article

    Leopoldo et al Nature. 2009 Jan 18.

    Promoter

    CAGG

    Tags

    Venus

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-hSyn-mCherry (AAV9)

    Viral Prep

    #114472-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-mCherry (#114472). In addition to the viral particles, you will also receive purified pAAV-hSyn-mCherry plasmid DNA. Synapsin-driven mCherry control vector. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    Syn

    Tags

    mCherry

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

18. pCIS GEM-CEPIA1er

    Plasmid

    #58217

    Purpose

    Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    GEM-CEPIA1er
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GEM-GECO1 E298D F359W D400E E407D

    Promoter

    CAG

    Available Since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-NR1a

    Plasmid

    #17926

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (Grinl1a Rat)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cytoplasmic cpGFP

    Plasmid

    #215709

    Purpose

    Expresses cpGFP in the cytoplasm

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    cpGFP
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and cytoplasmic localization sequence

    Expression

    Mammalian

    Mutation

    circularly permuted GFP

    Promoter

    CMV

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLSB-NAT

    Plasmid

    #166698

    Purpose

    Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.

    Depositor

    Robin Allshire

    Article

    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

    Inserts

    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    

    Expression

    Yeast

    Promoter

    adh15 promoter and tRNA promoter

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pEGFP-D50 lamin A

    Plasmid

    #17653

    Depositor

    Tom Misteli

    Article

    Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

    Insert

    D50 lamin A (LMNA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta 50 (50 aa internal deletion in globular tai…

    Promoter

    CMV

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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23. Nuclear cpGFP

    Plasmid

    #215707

    Purpose

    Expresses cpGFP in the nucleus

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    cpGFP
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and NLS

    Expression

    Mammalian

    Mutation

    circularly permuted GFP

    Promoter

    CMV

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) (AAV PHP.eB)

    Viral Prep

    #199776-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) (#199776). In addition to the viral particles, you will also receive purified AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) plasmid DNA. Cre recombinase expression in striatal direct pathway medium spiny neurons (D1-MSNs). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

25. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (AAV5)

    Viral Prep

    #62723-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (#62723). In addition to the viral particles, you will also receive purified pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] plasmid DNA. Cre-dependent ChrimsonR-tdTomato under the control of the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Promoter

    Syn

    Tags

    tdTomato (Cre-dependent)

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

26. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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27. pBad-EBFP2

    Plasmid

    #14891

    Purpose

    Bacterial expression of EBFP2 blue fluorescent protein

    Depositor

    Robert Campbell

    Article

    Ai et al Biochemistry. 2007 May 22;46(20):5904-10

    Has Service

    Cloning Grade DNA

    Insert

    EBFP2
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    The fluorescent protein was inserted between XhoI…

    Available Since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCDNA3.1(+)-CMV-bArrestin2-TEV

    Plasmid

    #107245

    Purpose

    Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    ARRB2
    

    Tags

    TEV(NIA), tobacco etch virus NIA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pcDNA-DEST47 PINK1 C-GFP

    Plasmid

    #13316

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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30. qTAG-C-dTAG-Zeo

    Plasmid

    #207738

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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31. pDB5318

    Plasmid

    #204828

    Purpose

    An integrating plasmid containing both the inducible enotetS promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Expression

    Yeast

    Promoter

    CMV promoter and enotetS promoter

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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32. pENN.AAV.cTNT.PI.eGFP.WPRE.rBG (AAV9)

    Viral Prep

    #105543-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pENN.AAV.cTNT.PI.eGFP.WPRE.rBG (#105543). In addition to the viral particles, you will also receive purified pENN.AAV.cTNT.PI.eGFP.WPRE.rBG plasmid DNA. cTNT-driven eGFP. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    cTNT

    Tags

    EGFP

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

33. AAV-cfGFP

    Plasmid

    #200773

    Purpose

    Expresses cysteine-free GFP (cfGFP) under synapsin promoter

    Depositor

    Alexander Dityatev

    Article

    Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

    Insert

    cysteine-free GFP
    

    Use

    AAV

    Promoter

    Synapsin

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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34. pSIRV-NFAT-eGFP

    Plasmid

    #118031

    Purpose

    Fluorescent reporter for NFAT activation

    Depositor

    Peter Steinberger

    Article

    Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

    Insert

    eGFP
    

    Use

    Retroviral

    Promoter

    minimal promoter with NFAT binding site

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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35. pTRKH3-slpGFP

    Plasmid

    #27168

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
    

    Expression

    Bacterial

    Available Since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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36. Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

    Plasmid

    #173029

    Purpose

    For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

    Depositor

    Mohamed Fareh

    Article

    Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

    Insert

    pspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, BFP, HIV NES, and T2A

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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37. pNZ18-CAH

    Plasmid

    #203159

    Purpose

    Replicates in E. coli, S. cerevisiae, and A. laidlawii

    Depositor

    Bogumil Karas

    Article

    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

    Insert

    Yeast centromere, autonomously replicating sequence, histidine auxotrophic marker
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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38. pET259-pUC57 24xMS2V6

    Plasmid

    #104391

    Purpose

    MS2V6 cloning

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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39. Synaptojanin2-pmCherryC1

    Plasmid

    #27677

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Synaptojanin 2 (Synj2 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

40. pMD1 anti-GFP nanobody-Tn5

    Plasmid

    #215567

    Purpose

    Expresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tag

    Depositor

    Isabelle Mansuy

    Article

    Maria A. et al bioRxiv 2024.07.12.603224

    Insert

    anti-GFP nanobody-Tn5 fusion
    

    Tags

    3xFlag and intein-CBD

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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