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Showing: 2801 - 2850 of 3030 results

  1. pVZ_PcoaT_SQE1_MRN1

    Plasmid
    #103858
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and MRN1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    marneral synthase 1 (MRN1 Mustard Weed)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized2nd amino acid …
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    103858-plasmid-map-sequence-id-202616

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  2. pVZ_PcoaT_SQE1_CAS1

    Plasmid
    #103856
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and CAS1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    cycloartenol synthase 1 (CAS1 Mustard Weed)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    103856-plasmid-map-sequence-id-202299

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  7. STARR-seq luciferase validation vector_ORI_AGAP1

    Plasmid
    #99300
    Purpose
    Luciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Insert
    enhancer; chr2: 236735694-236737193 (AGAP1 Human)
    Use
    Luciferase; Starr-seq luciferase validation vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    99300-plasmid-map-sequence-id-190939

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  8. STARR-seq luciferase validation vector_mP_AGAP1

    Plasmid
    #99301
    Purpose
    Luciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Insert
    enhancer; chr2: 236735694-236737193 (AGAP1 Human)
    Use
    Luciferase; Starr-seq luciferase validation vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    99301-plasmid-map-sequence-id-193155

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  9. STARR-seq luciferase validation vector_SCP1_AGAP1

    Plasmid
    #99302
    Purpose
    Luciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Insert
    enhancer; chr2: 236735694-236737193 (AGAP1 Human)
    Use
    Luciferase; Starr-seq luciferase validation vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    99302-plasmid-map-sequence-id-193011

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  15. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid
    #108362
    Purpose
    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans
    Depositor
    Luisa Cochella
    Article
    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461
    Inserts
    Ath-HEN1 (HEN1 Codon optimised for C. elegans, Mustard Weed)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    Use
    Tags
    2xFlag, MYC, T2A peptide, and TY1 linker
    Expression
    Worm
    Mutation
    Promoter
    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…
    Available since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    108362-plasmid-map-sequence-id-209815

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  19. pPHR-mCherry-FUSN-mHP1alpha

    Plasmid
    #181892
    Purpose
    Soluble light-dependent effector protein (HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN) ) for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-FUSN-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    Use
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181892-plasmid-map-sequence-id-359691

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  20. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid
    #186427
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Rb7
    eGFP
    Use
    Synthetic Biology; Binary vector for escherichia …
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186427-plasmid-map-sequence-id-369354

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  22. pPHR-mCherry-nE-mHP1alpha

    Plasmid
    #181890
    Purpose
    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant) for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-nE-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    Use
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Mutation
    S11E, S12E, S13E, S14E
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181890-plasmid-map-sequence-id-359697

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  23. pVQ CMV NanoV1-2a-EGFP ferritin

    Plasmid
    #79649
    Purpose
    Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein
    Depositor
    Jeffrey Friedman
    Article
    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do
    Inserts
    transient receptor potential vanillin 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    79649-plasmid-map-sequence-id-175067

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  24. pVQ CMV NanoV1Mutant-2a-EGFP ferritin

    Plasmid
    #79650
    Purpose
    Expresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion protein
    Depositor
    Jeffrey Friedman
    Article
    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do
    Inserts
    transient receptor potential vanilloid 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    isoleucine 679 changed to lysine
    Promoter
    CMV
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    79650-plasmid-map-sequence-id-156910

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  25. pPHR-mCherry-RGG-GFP-RGG-mHP1alpha

    Plasmid
    #181893
    Purpose
    Soluble light-dependent effector protein (HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1) for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-RGG-GFP-RGG-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    Use
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181893-plasmid-map-sequence-id-359709

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  26. pPHR-mCherry-nE-mHP1alpha-delCTE

    Plasmid
    #181891
    Purpose
    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mouse, Mustard Weed)
    Use
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Mutation
    S11E, S12E, S13E, S14E, 14 aa C-terminal deletion
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181891-plasmid-map-sequence-id-359705

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  27. pGENT_GW_aco_mScarlet3

    Plasmid
    #225991
    Purpose
    To make gene fusions with Arabidopsis codon-optimized mScarlet3
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    Tags
    mScarlet3
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225991-plasmid-map-sequence-id-448012

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  28. pJKW0474

    Plasmid
    #107588
    Purpose
    pCLV3:Cas9
    Depositor
    Jing-Ke Weng
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis CLAVATA3
    Available since
    April 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    107588-plasmid-map-sequence-id-208235

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  29. pMJA251

    Plasmid
    #128540
    Purpose
    Acceptor vector for gamma and Delta TCR
    Depositor
    Marcos Alcocer
    Insert
    truncated human TCR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional CMV
    Available since
    July 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    128540-plasmid-map-sequence-id-251681

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  30. pMJA252

    Plasmid
    #128541
    Purpose
    Acceptor vector for alpha and beta TCR
    Depositor
    Marcos Alcocer
    Insert
    truncated human TCR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional CMV
    Available since
    July 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    128541-plasmid-map-sequence-id-251633

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  31. pMJA295

    Plasmid
    #128543
    Purpose
    Expresses nut lipid binding TCR
    Depositor
    Marcos Alcocer
    Insert
    Lipid binding Human TCR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional CMV
    Available since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    128543-plasmid-map-sequence-id-251874

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  32. pMJA297

    Plasmid
    #128544
    Purpose
    Expresses nut lipid binding TCR
    Depositor
    Marcos Alcocer
    Insert
    Lipid binding Human TCR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional CMV
    Available since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    128544-plasmid-map-sequence-id-251616

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  33. pMJA290

    Plasmid
    #128542
    Purpose
    Expresses nut lipid binding TCR
    Depositor
    Marcos Alcocer
    Insert
    Lipid binding Human TCR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional CMV
    Available since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    128542-plasmid-map-sequence-id-251560

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  34. BII-tdT-inv-GFP

    Plasmid
    #133393
    Purpose
    Piggybac vector for bidirectional promoter reporter assay
    Depositor
    Blair Madison
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    No promoter
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133393-plasmid-map-sequence-id-268036

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  35. pHEX2 T494 TALENs

    Plasmid
    #44464
    Depositor
    Ross Johnson, Roger Hellens
    Insert
    AtCRU3 T494 TALENs
    Use
    Plant transformation
    Tags
    Expression
    Mutation
    Promoter
    CaMV 35S
    Available since
    May 6, 2013
    Availability
    Academic Institutions and Nonprofits only

  36. pLSU1/t35s[AtPsaK]:MCS+:NosT

    Plasmid
    #212159
    Purpose
    This binary vector has an empty backbone where you can insert a gene into a cassette with a strong promoter (truncated 35sCAMV) and UTR (truncated Arabidopsis thaliana PsaK)
    Depositor
    Wayne Curtis
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212159-plasmid-map-sequence-id-422849

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  37. Ef1a-somBiPOLES-mCerulean-IRES-Puro

    Plasmid
    #192585
    Purpose
    lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    Lentiviral
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-alpha
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192585-plasmid-map-sequence-id-379281

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  38. TRE3GV-somBiPOLES-mKate2

    Plasmid
    #192579
    Purpose
    AAV-vector for bidirectional optogenetic manipulation of tTA-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    AAV
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3GV
    Available since
    Dec. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    192579-plasmid-map-sequence-id-379367

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  39. TRE3GV-somBiPOLES-mCerulean

    Plasmid
    #192578
    Purpose
    AAV-vector for bidirectional optogenetic manipulation of tetTag-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    AAV
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3GV
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192578-plasmid-map-sequence-id-379291

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  40. CaMKII-somBiPOLES-eGFP

    Plasmid
    #192583
    Purpose
    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    AAV
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa(0.4)
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192583-plasmid-map-sequence-id-379280

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  41. 6OGN

    Plasmid
    #162260
    Purpose
    Bacterial Expression
    Depositor
    Cheryl Arrowsmith
    Insert
    PRMT7 (PRMT7 Human)
    Use
    Baculovirus expresssion
    Tags
    6xHis-TEV
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162260-plasmid-map-sequence-id-319822

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  42. hSyn-DIO-somBiPOLES-eGFP

    Plasmid
    #192584
    Purpose
    AAV-vector for bidirectional optogenetic manipulation of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    AAV
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192584-plasmid-map-sequence-id-379290

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  43. CRY2-OCRL

    Plasmid
    #79566
    Purpose
    expression of CRY2PHR fused to inositol 5-phosphatase domain of OCRL
    Depositor
    Olof Idevall-Hagren
    Insert
    OCRL (OCRL Human)
    Use
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    aa 234_539
    Promoter
    CMV
    Available since
    Aug. 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  44. CaMKII-somBiPOLES-ChroME2s-mCerulean

    Plasmid
    #192582
    Purpose
    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    AAV
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa(0.4)
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192582-plasmid-map-sequence-id-379296

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  45. CaMKII-somBiPOLES-ChroME2f-mCerulean

    Plasmid
    #192581
    Purpose
    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    AAV
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa(0.4)
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192581-plasmid-map-sequence-id-379294

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  46. pMDC32_PbGH3

    Plasmid
    #232335
    Purpose
    Generation of overexpressing Arabidopsis thaliana and Brassica napus lines to characterize the role of PbGH3 in plant.
    Depositor
    Edel Pérez-López
    Insert
    PbGH3
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232335-plasmid-map-sequence-id-463036

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  49. pGWB602-oeUnaG

    Plasmid
    #221662
    Purpose
    For Agrobacterium-mediated transformation. Plastid(outer envelope membrane)-localized mCherry-UnaG.
    Depositor
    Yutaka Kodama
    Article
    Ishikawa et al Plant Cell Physiol. 2024 Feb 27:pcae017.
    Insert
    mCherry-UnaG flanked with an N-terminal sequence of Arabidopsis OUTRE ENVELOPE PROTEIN7
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    221662-plasmid-map-sequence-id-449159

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  50. pGWB602-tkUnaG

    Plasmid
    #221661
    Purpose
    For Agrobacterium-mediated transformation. Plastid(thylakoid membrane)-localized mCherry-UnaG.
    Depositor
    Yutaka Kodama
    Article
    Ishikawa et al Plant Cell Physiol. 2024 Feb 27:pcae017.
    Insert
    mCherry-UnaG flanked with the full-length coding sequence for Arabidopsis BESTROPHIN-LIKE PROTEIN2
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    221661-plasmid-map-sequence-id-448723

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Showing: 2801 - 2850 of 3030 results