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Plasmid#191371PurposeOverexpression of 3xGFP-tDeg in human cellsDepositorInsert3xGFP-tDeg
UseTags3xGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-Halo-tDeg
Plasmid#191370PurposeOverexpression of Halo-tDeg in human cellsDepositorInsertHalo-tDeg
UseTagsHaloExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NLS-dMisCas13b-SNAPf-NLS-3xFlag
Plasmid#191536PurposeOverexpression of NLS-dMisCas13b-SNAPf-NLS-3xFlag in human cellsDepositorInsertNLS-dMisCas13b-SNAPf-NLS-3xFlag
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dMisCas13b-EGFP-NLS
Plasmid#191359PurposeOverexpression of NLS-dMisCas13b-EGFP-NLS in human cellsDepositorInsertNLS-dMisCas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC24A2_STOP
Plasmid#161132PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC24A2 (SLC24A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-T7-AcrIIC1-NLS(SV40) (KAC208)
Plasmid#134340PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIC1 with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIC1
UseTagsNLS(SV40)ExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV1)
Viral Prep#37825-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
hFerritinH
Plasmid#122652PurposeExpression of hFerritinH in bacterial cellsDepositorInsertferritin heavy chain 1 (FTH1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
RpHLuorin2
Plasmid#180227Purposefor bacterial expression of recombinant ratiometric pHluorin2DepositorInsertRatiometric pHLuorin2
UseTagsHis-tag followed by thrombin cleavage siteExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-rDA3h
Plasmid#208703PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neuronsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA3h
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PE2max-BSD
Plasmid#191102PurposeLentiviral expression plasmid of PEmax prime editor with P2A-BSD markerDepositorInsertPEmax-P2A-BSD
UseCRISPR and LentiviralTagsExpressionMammalianMutationChanged R221K, N394K, H840A from SpCas9PromoterEF-1aAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
hCtRNA_FT
Plasmid#186715PurposeFragment template for DAP arrayDepositorInsertgRNA scaffold and hCtRNA
UseCRISPRTagsExpressionMammalianMutationPromoterhCtRNAAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpRY Cas9
Plasmid#188491PurposeExpresses FLAG tagged SpRY Cas9DepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationA61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…PromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-Puro-dTAG (BRD4)
Plasmid#91793PurposePITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorInsertPuro-P2A-2xHA-FKBP_F36V
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2‐Tsa2ΔCR
Plasmid#83238PurposeThe genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCr
UseTagsroGFP2 fusion with Tsa2dCrExpressionYeastMutationPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCH67 (crRNA expression vector)
Plasmid#217337PurposeCROP-seq crRNA expression vector with GFP and puro markerDepositorInsertAsDR-filler-AsDR8, GFP-puro
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFJ782 - HygroR
Plasmid#190933PurposeHygromycin resistance co-injection markerDepositorInsertHygromycin resistance gene
UseTagsExpressionWormMutationPromoterrps-0Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX304-KDM6A
Plasmid#203580PurposeExpresses wild-type KDM6A in mammalian cells.DepositorInsertKDM6A (KDM6A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T7-ARG1
Plasmid#106473PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coliDepositorInsertARG1
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GFP(1-10)
Plasmid#70219PurposeExpresses GFP(1-10) in mammalian cellsDepositorHas ServiceCloning Grade DNAInsertGFP(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSMPP-mCherry-hTRIM21
Plasmid#104972PurposeLentiviral Transfer Plasmid with mCherry-tagged human TRIM21DepositorInsertTripartite Motif containing 21 (TRIM21 Human)
UseLentiviralTagsmCherryExpressionMutationPromoterSFFVAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
dSpCas9-VPR
Plasmid#205247PurposeIn vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.DepositorInsertdSpCas9-VPR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.27_ARE/NRF2-SPE
Plasmid#177775PurposeLuciferase reporter for ARE/NRF2DepositorInsertNRF2 (NFE2L2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/1
Plasmid#112862PurposeAAV packaging plasmid expressing Rep/Cap genesDepositorInsertRep2/Cap1
UseAAVTagsExpressionMutationPromoterTruncated P5Available sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mitochondrial cpGFP
Plasmid#215711PurposeExpresses cpGFP in the mitochondriaDepositorInsertcpGFP
UseLentiviralTagsFLAG, HA, and mitochondrial localization sequence…ExpressionMammalianMutationcircularly permuted GFPPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-ASAP5-WPRE
Plasmid#225709PurposeCre-dependent pan-membrane expression of the genetically encoded voltage indicator ASAP5, can be used for dendritic voltage imagingDepositorInsertASAP5
UseAAVTagsExpressionMutationPromoterhuman synapsinAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_NUP98_KDM5A
Plasmid#205872PurposeExpress mEGFP-tagged fusion protein, NUP98_KDM5A from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
Plasmid#71236PurposeExpress sgRNA and dCas9-KRAB from lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A Puro
sgRNA
UseCRISPR and LentiviralTagsFlagExpressionMutationD10A and H840APromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Blast
Plasmid#199622PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
KRAB-dSpCas9
Plasmid#205248PurposeIn vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.DepositorInsertKRAB-dSpCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac PlayBack
Plasmid#203312PurposeTo all for PiggyBac gene insertion into GenomeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-Opt WWP1
Plasmid#179327PurposeE. coli optimized expression of GST-fusion of human WWP1DepositorInsertWWP1 (WWP1 Human)
UseTagsGSTExpressionBacterialMutationCodon optimized for expression in E. coliPromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Con/Fon EYFP (AAV8)
Viral Prep#55650-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn Con/Fon EYFP (#55650). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon EYFP plasmid DNA. Synapsin driven, Cre and Flp-dependent EYFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFP (Cre- and Flp-dependent)Available sinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
PRESTO-Tango Kit
Plasmid Kit#1000000068PurposeUsed to measure receptor activation for more than 300 G-protein coupled receptors (GPCRs) via a modified Tango beta-arrestin recruitment assay.DepositorAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon-ChRmine-oScarlet (AAV8)
Viral Prep#137160-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChRmine-oScarlet (#137160). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChRmine-oScarlet plasmid DNA. nEF-driven expression of ChRmine-oScarlet in the presence of Flp (inhibited in the presence of Cre). These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsoScarlet (Flp-dependent)Available sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6f
Plasmid#40755PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertGCaMP6f
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-FLEx-axon-GCaMP6s (AAV9)
Viral Prep#112010-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSynapsin1-FLEx-axon-GCaMP6s (#112010). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-FLEx-axon-GCaMP6s plasmid DNA. Synapsin-driven, cre-dependent axon-targeted GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV Retrograde)
Viral Prep#20297-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-dependent)Available sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-G-Flamp1
Plasmid#188567Purposegreen biosensor for cAMP in mammalian cellsDepositorInsertG-Flamp1
UseTagsHis tagExpressionMammalianMutationPromoterCAGAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
EB3-tdTomato
Plasmid#50708Purposeexpresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTsDepositorInsertEB3 (MAPRE3 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only