167,948 results

1. pBBR1_DVK_AE(J23100-BCD2-E0040m-B0015)

   Plasmid

   #197660

   Purpose

   Expresses GFP under the control of J23100 and BCD2

   Depositor

   Eric Young

   Article

   Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-53-RtcB

   Plasmid

   #51282

   Purpose

   E. coli expression vector for E. coli RtcB RNA ligase

   Depositor

   Jay Hesselberth

   Article

   Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

   Insert

   E. coli RtcB
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7 RNA Polymerase

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTRIP-SFFV-EGFP-NLS-FLAG-cGAS

   Plasmid

   #127659

   Purpose

   Over-express EGFP-NLS-FLAG-cGAS

   Depositor

   Nicolas Manel

   Article

   Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

   Insert

   cGAS (CGAS Human)
   

   Use

   Lentiviral

   Tags

   EGFP-NLS-Flag

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Human Lentiviral sgRNA Library - Kinases

   Pooled Library

   #51044

   Purpose

   Sub-pool of Sabatini/Lander sgRNA library enriched for kinase targets.

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pBS-slpGFP

   Plasmid

   #27171

   Depositor

   Michela Lizier

   Article

   Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

   Insert

   surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
   

   Expression

   Bacterial

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCLucf

   Plasmid

   #37328

   Depositor

   John Schiller

   Article

   Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 20

   Inserts

   Luciferase (luciferase Fly)
   GFP
   

   Expression

   Mammalian

   Promoter

   CMV and SV40

   Available Since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMW#3

   Plasmid

   #13350

   Depositor

   Marian Walhout

   Article

   Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

   Insert

   LacZ reporter
   

   Expression

   Yeast

   Available Since

   Nov. 9, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBabe-GFP-IKBalpha-wt

   Plasmid

   #15263

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   IKB alpha (NFKBIA Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1_nV5-AMOT (p130)

   Plasmid

   #172997

   Purpose

   constitutive expression of V5-tagged AMOT (p130)

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   V5-AMOT (p130) (AMOT Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET3a aSyn murine

    Plasmid

    #108865

    Purpose

    Expresses murine alpha synuclein

    Depositor

    Gabriele Kaminski Schierle

    Insert

    alpha synuclein (Snca Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti_CMV_FR-GECO1c

    Plasmid

    #224358

    Purpose

    Expresses red fluorescent calcium sensor FR-GECO1c

    Depositor

    Adam Cohen

    Article

    Hayward et al Cell Calcium. 2024 Dec 5;125:102983. doi

    Insert

    FR-GECO1c
    

    Use

    Lentiviral

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMW#2

    Plasmid

    #13349

    Depositor

    Marian Walhout

    Article

    Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

    Insert

    HIS3 reporter
    

    Expression

    Yeast

    Available Since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHiUGE TurboID-HA donor (all-in-one) ORF0

    Plasmid

    #200383

    Purpose

    Tagging endogenously expressed proteins with TurboID-HA, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pL6puro-JeT-pmRevErbA-Venus-NLS-Pest

    Plasmid

    #240106

    Purpose

    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.

    Depositor

    Achim Kramer

    Article

    Gabriel et al bioRxiv 2025.06.30.661926

    Insert

    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    

    Use

    Lentiviral

    Mutation

    added JeT Promoter

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pL6puro-pSV40(1)-pmRevErbA-Venus-NLS-Pest

    Plasmid

    #240112

    Purpose

    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.

    Depositor

    Achim Kramer

    Article

    Gabriel et al bioRxiv 2025.06.30.661926

    Insert

    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    

    Use

    Lentiviral

    Mutation

    added SV40-Enhancer

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (AAV5)

    Viral Prep

    #35509-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (#35509). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Promoter

    EF1a

    Tags

    EYFP (Cre-dependent)

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pGMC00028 (aka pTS0057)

    Plasmid

    #200832

    Purpose

    AAVS1 guide RNA cloned into the lentiSAM puro backbone

    Depositor

    Raj Chari

    Article

    Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

    Insert

    AAVS1 guide RNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psgRNAc

    Plasmid

    #114006

    Purpose

    express sgRNA, p15A, CRM

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Insert

    gRNA
    

    Promoter

    BBa_J23119

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. DRD2-3xHA/pCDNA3.1

    Plasmid

    #196940

    Purpose

    Plasmid expressing an antigen targeting the D2 dopamine receptor

    Depositor

    James Trimmer

    Insert

    DRD2 (DRD2 Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AICSDP-45: TTN-mEGFP

    Plasmid

    #114412

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    TTN Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TTN Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid

    #160729

    Purpose

    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FLAG-HA-FbxO11-pcDNA3.1-

    Plasmid

    #52501

    Purpose

    expresses human FbxO11 in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA Flag-RARalpha

    Plasmid

    #35555

    Depositor

    Harish Srinivas, Jonathan Kurie

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Tags

    Flag and His

    Expression

    Mammalian

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pIW601-KmCRISPR

    Plasmid

    #98907

    Purpose

    K. marxianus CRISPR Plasmid for sgRNA cloning

    Depositor

    Ian Wheeldon

    Article

    Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

    Inserts

    Codon optimized Cas9
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET16better_H10_HRV3C_chitinase

    Plasmid

    #175091

    Purpose

    Expresses B. licheniformis chitinase with an N terminal 10X His tag and HRV-3C cleavage site in E. coli.

    Depositor

    Jay C Dunlap

    Article

    Bartholomai et al Fungal Genet Biol. 2021 Aug 20;156:10361

    Insert

    chitinase
    

    Tags

    10X His, HRV-3C

    Expression

    Bacterial

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT22-GluCNZ-6His

    Plasmid

    #213504

    Purpose

    Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.

    Depositor

    John Denu

    Article

    Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12

    Insert

    Glucanase (glucan endo-1,3-beta-glucosidase)
    

    Tags

    6His tag

    Expression

    Bacterial

    Mutation

    1-36 Signal Sequence Deletion

    Promoter

    T7

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. His6_MBP_FMRP_P451S

    Plasmid

    #198693

    Purpose

    Expression of human FMRP protein in E. coli

    Depositor

    Simpson Joseph

    Article

    Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

    Insert

    FMR1 (FMR1 Human)
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed proline 451 to serine

    Promoter

    T7

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSmac-GFP

    Plasmid

    #40881

    Depositor

    Douglas Green

    Article

    Tait et al Dev Cell. 2010 May 18;18(5):802-13.

    Insert

    Smac-GFP (DIABLO Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

29. hCathepsin S

    Plasmid

    #11251

    Purpose

    Mammalian expression of human Cathepsin S

    Depositor

    Hyeryun Choe

    Article

    Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

    Insert

    cathepsin S (CTSS Human)
    

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

30. pEGFP×2-N1

    Plasmid

    #86775

    Purpose

    A mammalian expression vector with double EGFP at C-terminus.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDNA3 Flag Jnk1a1

    Plasmid

    #13798

    Depositor

    Roger Davis

    Article

    Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

    Insert

    Jnk1a1 (MAPK8 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMSCVpuro-Venus

    Plasmid

    #96940

    Purpose

    Retroviral plasmid containing Venus for labeling of cells

    Depositor

    Lee Grimes

    Article

    Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

    Insert

    Venus Fluorescent Protein
    

    Expression

    Mammalian

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p3xFLAG SARS-CoV2 RdRp.co

    Plasmid

    #154008

    Purpose

    Expression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cells

    Depositor

    Patrick Moore

    Article

    Meng et al J Med Virol. 2023 Jan;95(1):e28246. doi:

    Insert

    Codon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)
    

    Tags

    3xFLAG tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pCMV-TadA8e-BLM

    Plasmid

    #221226

    Purpose

    Express HACE Editor as a fusion of TadA-8e adenosine deaminase and BLM helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-BLM
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pVITRO-HPV33 L1L2

    Plasmid

    #52493

    Purpose

    Expresses HPV33 L1 and L2 in mammalian cells

    Depositor

    Richard Roden

    Article

    Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

    Inserts

    HPV33 L1
    HPV33 L2
    

    Expression

    Mammalian

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDONR223_TMPO_WT

    Plasmid

    #81818

    Purpose

    Gateway Donor vector containing TMPO , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TMPO (TMPO Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PET-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207376

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGL-p21UTR

    Plasmid

    #20014

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR (Cdkn1a Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

39. pX459-sgAAVS1

    Plasmid

    #184403

    Purpose

    pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    AAVS1-specific sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV.CAG.iGluSnFR3.v857.PDGFR

    Plasmid

    #178333

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge