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161,697 results

Showing: 3051 - 3100 of 161697 results

  5. p6XHis-T7(P266L)

    Plasmid
    #174866
    Purpose
    Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.
    Depositor
    Anna Pyle
    Article
    Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.
    Insert
    T7 RNA polymerase
    Use
    Tags
    6XHis-tag
    Expression
    Bacterial
    Mutation
    P266L
    Promoter
    lac promoter
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174866-plasmid-map-sequence-id-419578

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  6. pCXLE-SOX2-17-p2a-KLF4

    Plasmid
    #193290
    Purpose
    episomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversion
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    SOX2-17-p2a-KLF4 (SOX2, KLF4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SOX2-17 is engineered highly cooperative chimeric…
    Promoter
    CAG
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    193290-plasmid-map-sequence-id-379665

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  7. pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (AAV9)

    Viral Prep
    #83897-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (#83897). In addition to the viral particles, you will also receive purified pAAV-hDlx-GqDREADD-dTomato-Fishell-4 plasmid DNA. hDlx-driven expression of GqDREADD (hM3D) and nuclear localized dTomato (physically separate) for activation of interneurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    hDlx
    Tags
    dTomato
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only

  12. pAAV-hSyn-hChR2(H134R)-EYFP (AAV1)

    Viral Prep
    #26973-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    EYFP
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  16. PB-TO-oNGN2

    Plasmid
    #198397
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Inserts
    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…
    Promoter
    CAG, EF1a, and TRE3G
    Available since
    April 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    198397-plasmid-map-sequence-id-389535

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  17. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid
    #213030
    Purpose
    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213030-plasmid-map-sequence-id-422062

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  18. mCherry-Golgi-7

    Plasmid
    #55052
    Purpose
    Localization: Golgi Complex, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    55052-plasmid-map-sequence-id-352458

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  21. pSIJ8

    Plasmid
    #68122
    Purpose
    Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression
    Depositor
    Alex Nielsen
    Article
    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103
    Inserts
    flp recombinase
    rhaS
    rhaR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rhamnose
    Available since
    Dec. 8, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    68122-plasmid-map-sequence-id-243302

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  23. pAAV-EF1a-fDIO-Cre (AAV8)

    Viral Prep
    #121675-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Esteban Engel, Alexander Nectow
    Article
    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30
    Promoter
    EF1a
    Tags
    None
    Available since
    Dec. 15, 2020
    Availability
    Academic Institutions and Nonprofits only

  32. pAAV.CMV.PI.EGFP.WPRE.bGH (AAV2)

    Viral Prep
    #105530-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    EGFP
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  35. Anti-GFP [N86/38.1R] (Recombinant Antibody)

    Antibody
    #180084-rAb
    Purpose
    Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody. Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively).
    Depositor
    James Trimmer
    Article
    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1
    Recommended Applications
    Immunocytochemistry, Immunohistochemistry, and Western Blot
    Reactivity
    Synthetic
    Source Species
    Mouse
    Isotype
    IgG2a
    Trial Size
    Available to purchase
    Available since
    March 29, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    Immunocytochemistry image by Addgene

  36. pAAV-CAG-GFP (AAV2)

    Viral Prep
    #37825-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    GFP
    Available since
    Oct. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  37. TRUPATH

    Plasmid Kit
    #1000000163
    Purpose
    A collection of 20 plasmids encoding alpha, beta, or gamma subunits of the heterotrimeric G protein complex for detection of heterotrimer dissociation. GPCR
    Depositor
    Bryan Roth
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only

  38. pSL1-GFP

    Plasmid
    #180420
    Purpose
    Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.
    Depositor
    Nancy Moran
    Article
    Li et al Proc Natl Acad Sci U S A. 2022 May 3;119
    Insert
    GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    180420-plasmid-map-sequence-id-357217

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  41. pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (AAV5)

    Viral Prep
    #100835-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (#100835). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project, Douglas Kim
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    CAG
    Tags
    None
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only

  42. pENN.AAV.CMVs.Pl.Cre.rBG (AAV9)

    Viral Prep
    #105537-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    None
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  48. Mouse sgRNA library Brie in lentiGuide-Puro (Lentiviral Prep)

    Viral Prep
    #73633-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Mouse sgRNA library Brie in lentiGuide-Puro (#73633). In addition to the viral particles, you will also receive purified Mouse sgRNA library Brie in lentiGuide-Puro plasmid DNA. <p>&lt;p&gt;Ready-to-use lentiviral pooled library for CRISPR screening in mouse cells. Use on cells that are stably expressing Cas9 to make edits across 19,674 genes in the mouse genome.&lt;/p&gt;</p>
    Depositor
    David Root, John Doench
    Article
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Promoter
    Tags
    None
    Available since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 3051 - 3100 of 161697 results