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179,463 results

Showing: 3051 - 3100 of 179463 results

  1. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190034
    Purpose
    vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13d
    Expression
    Mammalian
    Mutation
    A134V,A140V,A141V,A143V
    Promoter
    CBh, SV40, hU6
    Available since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    190034-plasmid-map-sequence-id-371641

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  2. pAAV-Ef1a-fDIO EYFP (AAV9)

    Viral prep
    #55641-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Tags
    EYFP (Flp-dependent)
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only

  3. pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro

    Plasmid
    #141370
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp5 (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    12 Articles
    141370-plasmid-map-sequence-id-288077

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  6. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV Retrograde)

    Viral prep
    #112677-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Publication
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only

  7. pAAV-CAG-GFP (AAV Retrograde trial size)

    Viral prep
    #37825-AAVrg.T
    Purpose
    Ready-to-use AAV Retrograde trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. GFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    GFP
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only

  12. pcDNA-RLuc8

    Plasmid
    #87121
    Purpose
    Mammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.
    Depositor
    Sanjiv Sam Gambhir
    Publication
    Loening et al Protein Eng Des Sel. 2006 Sep;19(9):391-
    Insert
    Renilla Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    A55T, C124A, S130A, K136R, A143M, M185V, M253L, a…
    Promoter
    CMV
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    87121-plasmid-map-sequence-id-175252

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  20. pAAV-CaMKIIa-EGFP

    Plasmid
    #50469
    Purpose
    EGFP under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    EGFP
    Use
    AAV
    Tags
    N/A
    Promoter
    CaMKIIa
    Available since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74 Articles
    50469-plasmid-map-sequence-id-171178

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  36. pDest-566

    Plasmid
    #11517
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway Cloning
    Tags
    6xHis and MBP
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    28 Articles
    11517-plasmid-map-sequence-id-299808

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  41. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV8)

    Viral prep
    #105545-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    HI
    Tags
    EGFP
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  42. pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (AAV1)

    Viral prep
    #100835-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (#100835). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    CAG
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only

  43. pAAV.synP.DIO.EGFP.WPRE.hGH (AAV9)

    Viral prep
    #100043-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.synP.DIO.EGFP.WPRE.hGH (#100043). In addition to the viral particles, you will also receive purified pAAV.synP.DIO.EGFP.WPRE.hGH plasmid DNA. Synapsin-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ian Wickersham
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 3051 - 3100 of 179463 results