280,033 results

1. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV1)

   Viral Prep

   #162379-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   Syn

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

2. pCMV CEPIA2mt

   Plasmid

   #58218

   Purpose

   Green fluorescent indicator for calcium imaging in the mitochondria

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   CEPIA2mt
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GCaMP2 N263Y E282V M339L

   Promoter

   CMV

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV CEPIA2mt

   Plasmid

   #58218

   Purpose

   Green fluorescent indicator for calcium imaging in the mitochondria

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   CEPIA2mt
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GCaMP2 N263Y E282V M339L

   Promoter

   CMV

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiMPH v2

   Plasmid

   #89308

   Purpose

   lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Insert

   MS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   N55K in MS2

   Promoter

   EF1a

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiMPH v2

   Plasmid

   #89308

   Purpose

   lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Insert

   MS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   N55K in MS2

   Promoter

   EF1a

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV1)

   Viral Prep

   #87306-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Li Zhang

   Article

   Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

   Promoter

   EF1a

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

7. PB-TRE-dCas9-VPR

   Plasmid

   #63800

   Purpose

   SP-dCas9-VPR with doxycycline-inducible expression

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-TRE-dCas9-VPR

   Plasmid

   #63800

   Purpose

   SP-dCas9-VPR with doxycycline-inducible expression

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiGuide-Hygro-mTagBFP2

   Plasmid

   #99374

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a and hU6

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiGuide-Hygro-mTagBFP2

    Plasmid

    #99374

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a and hU6

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Human sgRNA library Brunello in lentiCRISPRv2 (Lentiviral Prep)

    Viral Prep

    #73179-LV

    Purpose

    Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiCRISPRv2 (#73179). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2 plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. This backbone contains SpCas9 and unique gRNAs, and can be used to make edits across 19,114 genes in the human genome. In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2.

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Available Since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-hSyn-DIO-mCherry (AAV2)

    Viral Prep

    #50459-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pMSCV_IRES_GFP_CD16a

    Plasmid

    #196189

    Purpose

    Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

    Depositor

    Ana Ruiz-Saenz

    Article

    Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

    Insert

    CD16a (FCGR3A Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    changed Phenylalanine 158 to Valine

    Promoter

    LTR

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mKeima-Red-Mito-7

    Plasmid

    #56018

    Purpose

    Localization: Mitochondria, Excitation: 440, Emission: 620

    Depositor

    Michael Davidson

    Insert

    Mito (COX8A Human)
    

    Tags

    mKeima-Red

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. mKeima-Red-Mito-7

    Plasmid

    #56018

    Purpose

    Localization: Mitochondria, Excitation: 440, Emission: 620

    Depositor

    Michael Davidson

    Insert

    Mito (COX8A Human)
    

    Tags

    mKeima-Red

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCasSA

    Plasmid

    #98211

    Purpose

    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

    Depositor

    Quanjiang Ji

    Article

    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCasSA

    Plasmid

    #98211

    Purpose

    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

    Depositor

    Quanjiang Ji

    Article

    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-mt-mKeima

    Plasmid

    #131626

    Purpose

    lentiviral expression of mitochondrial mKeima for generation of stable cell lines

    Depositor

    Richard Youle

    Article

    Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

    Insert

    mt-mKeima
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-mt-mKeima

    Plasmid

    #131626

    Purpose

    lentiviral expression of mitochondrial mKeima for generation of stable cell lines

    Depositor

    Richard Youle

    Article

    Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

    Insert

    mt-mKeima
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-EGFP (AAV8)

    Viral Prep

    #50465-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    EGFP

    Available Since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only