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Showing: 3451 - 3500 of 6613 results

  1. phage - ubc - nls - 2xmcp - mCherry - BirA*

    Plasmid
    #131136
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-2xMCP-mCherry-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131136-plasmid-map-sequence-id-255502

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  8. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670)

    Plasmid
    #100061
    Purpose
    AAV expression of ChR1-VChR1 Chimera fused to mCherry driven by Ef1a promoter
    Depositor
    Karl Deisseroth
    Has Service
    AAV9
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    EF1a
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100061-plasmid-map-sequence-id-194678

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  10. pWPT-ACC/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49229
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49229-plasmid-map-sequence-id-73647

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  12. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid
    #129048
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA8 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129048-plasmid-map-sequence-id-256223

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  13. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid
    #129041
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA1 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129041-plasmid-map-sequence-id-256196

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  14. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid
    #129036
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA8 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129036-plasmid-map-sequence-id-256165

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  16. pHR-mCherry-p2A-LIC1 C-terminal half

    Plasmid
    #74603
    Purpose
    expresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherry
    Depositor
    Ron Vale
    Article
    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554
    Insert
    full length human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    amino acids 389-523 of LIC1
    Promoter
    SFFV
    Available since
    May 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    74603-plasmid-map-sequence-id-141268

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  17. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79889
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79889-plasmid-map-sequence-id-150065

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  18. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

    Plasmid
    #129053
    Purpose
    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA1 (mouseTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129053-plasmid-map-sequence-id-256238

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  20. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid
    #129029
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA1 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129029-plasmid-map-sequence-id-256133

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  25. Cenp-B DBD INCENP TSS/AAA mCherry

    Plasmid
    #45234
    Purpose
    Target INCENP (with TSS mutated to AAA) to centromeres via CENP-B DNA binding domain (DBD)
    Depositor
    Michael Lampson
    Article
    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.
    Insert
    Cenp-B DBD, INCENP-ΔCEN with AAA mutation, mCherry
    Use
    Tags
    CENP-B DBD, DNA binding domain of CENP-B and mChe…
    Expression
    Mammalian
    Mutation
    INCENP C-terminal TSS mutated to AAA
    Promoter
    t7
    Available since
    Sept. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    45234-plasmid-map-sequence-id-84485

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  30. pWPT-UCU/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49232
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49232-plasmid-map-sequence-id-73653

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  31. pCru5-GGG/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49221
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    Viral LTR (or CMV)
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49221-plasmid-map-sequence-id-73631

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  32. pCru5-UCU/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49223
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    Viral LTR (or CMV)
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49223-plasmid-map-sequence-id-73635

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  39. pcDNA4TO F8.2(S54L+T127A)-T2A-mCherry

    Plasmid
    #225586
    Purpose
    Mammalian expression vector for Doxycycline inducible expression of F8.2(S54L+T127A) anti-tau nanobody with a self-cleaving T2A-mCherry fluorescent expression reporter
    Depositor
    William McEwan
    Article
    Benn et al Science. 2024 Aug 30;385(6712):1009-1016
    Insert
    F8.2(S54L+T127A)-T2A-mCherry
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    225586-plasmid-map-sequence-id-446527

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  40. pLV-SFFV-CALU-WPRE-UbC-mCherry

    Plasmid
    #225952
    Purpose
    Lentiviral vector plasmid expressing human calumenin (CALU) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CALU (CALU Human)
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225952-plasmid-map-sequence-id-446855

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  41. pLV-SFFV-LNPK-WPRE-UbC-mCherry

    Plasmid
    #225954
    Purpose
    Lentiviral vector plasmid expressing human lunapark (LNPK) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    LNPK (LNPK Human)
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225954-plasmid-map-sequence-id-446860

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  50. EF1a-HexaPro_Foldon-PDGFR-T2A-mCherry (pZYW079)

    Plasmid
    #194234
    Purpose
    Lentiviral expression vector expressing Jason McClellan lab's 6-proline Spike extracellular domain on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker
    Depositor
    Hana El-Samad
    Article
    Weinberg et al bioRxiv 2021.04.20.440678
    Insert
    EF1a-Spike ECD-PDGFR TMD-T2A-mCherry (S Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    194234-plasmid-map-sequence-id-383907

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Showing: 3451 - 3500 of 6613 results