We narrowed to 11,308 results for: ENA
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Plasmid#72873PurposeThe retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson AssemblyDepositorAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only
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pHeEf1a-mEGFP-NLS
Plasmid#205012PurposeThe vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
UseTardigrade expressionAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LacI-pLacI-pIII pm
Plasmid#218100PurposePhagemid that expresses LacI, and pIII under the Lac promoterDepositorInsertpIII
ExpressionBacterialPromoterLac promoterAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDawn-Ag43
Plasmid#107742PurposeExpresses Ag43 under the control of a blue-light transcriptional regulatorDepositorInsertAg43 (flu )
ExpressionBacterialMutationremoved all pstI cut sites with silent mutations,…PromoterpLambdaAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACE-GIRK2-S
Plasmid#172426PurposeExpression of full-length, human GIRK2 with C-terminal StrepTag IIDepositorAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-DAXX-His
Plasmid#175782PurposeDAXX protein for bacteria expression systemDepositorAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH3717
Plasmid#191477PurposePacr-2s-Arch::wCherry unc-54 3' UTR C.elegans A/B MN expression of Arch RFPDepositorAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
OMM-V5-LOV-Turbo
Plasmid#199665Purposeexpresses LOV-Turbo on the mammalian outer mitochondrial membrane, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsAKAP1 transmembrane domain and V5ExpressionMammalianPromoterTRE3GAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM
Plasmid#183150PurposeExpression cassettes for transcription factor induced by estradiol (Lex-ER-B112) or progesterone (ZPM)DepositorInsertsLex-ER-B112
Zif268 DBD - hPRLBD - MSN2AD
UseFor yeast genomic integrationAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD6B (full-length, aa 1-293)
Plasmid#61418PurposeExpresses human OTUD6B (full-length) in E. coli.DepositorAvailable SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEAK-Flag/INPP4B
Plasmid#24324DepositorInsertInositol Polyphosphate 4-Phosphatase Type II (INPP4B Human)
TagsFlag and HAExpressionMammalianMutationnoneAvailable SinceMay 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pX330a dCas9-KRAB
Plasmid#92361PurposeModified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.DepositorInsertdCas9-KRAB
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYY1704
Plasmid#198421PurposepBs3:RedNTF:tNOS. A pGreen based plasmid expressing the INTACT reporter RedNTF after the promoter of the pepper Bs3 gene (pBs3). The plasmid has spectinomycin resistance.DepositorInsertRedNTF
ExpressionPlantPromoterpBs3Available SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3-WHaloCaMP1a
Plasmid#205312PurposeExpression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression)DepositorInsertWHaloCaMP1a
UseZebrafish expressionAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-parkin W403A
Plasmid#45876DepositorAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM-mSox2-3xflag
Plasmid#206376PurposeExpresses 3xflag fused mouse SOX2 in mammalian cells, for lentivirus generation.DepositorAvailable SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1
Plasmid#107407PurposeLentiviral expression of gRNA resistant PBRM1DepositorAvailable SinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
SP-GFP-CD274-NU
Plasmid#121139PurposeMammalian expression of GFP tagged CD274DepositorAvailable SinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD2 (full-length, aa 1-348)
Plasmid#61409PurposeExpresses human OTUD2 (full-length) in E. coli.DepositorAvailable SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)
Plasmid#71719PurposeExpressing a GFP-tagged degron in the soma of C. elegansDepositorInsertauxin-responsive protein IAA17 (AXR3 Mustard Weed)
TagsEmGFPExpressionWormMutationminimal degron sequence (71-114aa) with start cod…Promotereef-1A.1 (eft-3)Available SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(4CNW)RS
Plasmid#222871PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Cyano-L-Tryptophan (4-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
hBACCS2-IRES-mCherry
Plasmid#72892PurposeMammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherryDepositorInserthBACCS2-IRES-mCherry
TagsIRES-mCherryExpressionMammalianPromoterCMVAvailable SinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-ZIKVA-GFP-puro
Plasmid#140090PurposeFluorescence activatable reporter of ZIKV NS2B-NS3 protease activityDepositorInsertZIKVA-GFP
UseLentiviral; Low expressionTags6XHis tag and GFPExpressionMammalianPromoterCMVAvailable SinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DENV2A-GFP-puro
Plasmid#140089PurposeFluorescence activatable reporter of DENV-2 NS2B-NS3 protease activityDepositorInsertDENV2A-GFP
UseLentiviral; Low expressionTags6XHis tag and GFPExpressionMammalianPromoterCMVAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_25H
Plasmid#91145PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
UseCRISPRExpressionPlantAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2303
Plasmid#91068PurposeModule B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
UseCRISPRPromoterCmYLCVAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSF107mT-GATEWAY-3'-Myc tag
Plasmid#67617PurposeA pCS2 based vector to generate a 3'-Myc tag using GATEWAY cloning technologyDepositorTypeEmpty backboneUseIn vitro transcription or translation off of an s…TagsC-terminal MycExpressionMammalianPromoterCMV and SP6Available SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-oScarlet-WPRE-BC12-pA
Plasmid#220776PurposeExpression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertoScarlert-WPRE-BC12
UseAAVPromoterCAGAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cas12j-8-puro-crRNA
Plasmid#194965PurposeThe plasmid expresses human codon-optimized Cas12j-8, crRNA expression elements and puromycin resistence gene.DepositorTypeEmpty backboneUseAAVExpressionMammalianAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
shPBRM1#32-Tet-pLKO-puro
Plasmid#107399PurposeLentiviral expression of shRNA targeting PBRMDepositorInsertshRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
shPBRM1#131-Tet-pLKO-puro
Plasmid#107400PurposeLentiviral expression of shRNA targeting PBRMDepositorInsertshRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-miniTurbo-YFP-NLS-STOP-L2
Plasmid#127362Purposegateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choiceDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, NLS, V5, and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES260-sc.eEF2 K509A
Plasmid#85120Purposeyeast expression of mutant EEF2DepositorTags6xHisExpressionYeastMutationK509APromoterGAL1Available SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Lenti-CAGGS-hPRDM6-2xGly-V5-EF1-Luc2-Blast
Plasmid#213826PurposeExpresses human PRDM6 with a C-terminal V5 epitope tag.DepositorAvailable SinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pTRIP-SFFV-GFP-RELA
Plasmid#196629PurposeExpress GFP-RELADepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21C
Plasmid#91130PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRExpressionPlantAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
h-Plekhm1_EGFP
Plasmid#73836PurposeExpress human Plekhm1 in mammalian cellsDepositorAvailable SinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcU6_3 sgRNA
Plasmid#92359PurposeChicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.DepositorInsertchick U6.3 promoter and gRNA cloning cassette
UseCRISPRExpressionMammalianPromoterchick U6.3Available SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
AA184
Plasmid#216047PurposeFragmid fragment: (C' terminus) for prime editing; using PEmaxDepositorHas ServiceCloning Grade DNAInsertSV40NLS_v1.2; MMLV-RT_v1.2; SV40NLS_v1.3; c-mycNLS_v1.1
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA063
Plasmid#216020PurposeFragmid fragment: (Cas protein) nickase Cas for prime editingDepositorHas ServiceCloning Grade DNAInsertnCas9-SpRY (H840A)_v1.1 [Sp]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA061
Plasmid#216018PurposeFragmid fragment: (Cas protein) nickase Cas for prime editingDepositorHas ServiceCloning Grade DNAInsertnCas9-NG (H840A)_v1.1 [Sp]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA062
Plasmid#216019PurposeFragmid fragment: (Cas protein) nickase Cas for prime editingDepositorHas ServiceCloning Grade DNAInsertnCas9-SpG (H840A)_v1.1 [Sp]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA060
Plasmid#216017PurposeFragmid fragment: (Cas protein) nickase Cas for prime editingDepositorHas ServiceCloning Grade DNAInsertnCas9 (H840A)_v1.1 [Sp]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgPBRM1-1- LentiCRISPRv2
Plasmid#107403PurposeLentiviral expression of Cas9 and gRNA targeting PBRM1DepositorInsertgRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgPBRM1-2- LentiCRISPRv2
Plasmid#107404PurposeLentiviral expression of Cas9 and gRNA targeting PBRM1DepositorInsertgRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-miniTurbo-L2
Plasmid#127352Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11
Plasmid#193752Purposehuman codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGHDepositorInsertEF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNLF1W
Plasmid#136404PurposeExpression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.DepositorTypeEmpty backboneUseLuciferaseTagsNanoLuc luciferaseExpressionMammalianPromoterCMVAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-phiC31-LP-TC
Plasmid#183755PurposeAAVS1 targeting construct with the phiC31 landing pad cassette.DepositorInsertFRT-mCherry-attP-BleoR-F3
UseSynthetic BiologyExpressionMammalianAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only