We narrowed to 8,813 results for: Ott

1. pAAV-Puro_siKO-2TO

   Plasmid

   #86697

   Purpose

   Tetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus

   Depositor

   Ludovic Vallier

   Article

   Bertero et al Development. 2016 Dec 1;143(23):4405-441

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 2TO

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-NES-NIR-GECO1

   Plasmid

   #113683

   Purpose

   Express NIR-GECO1 preferentially in neurons

   Depositor

   Robert Campbell

   Article

   Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

   Insert

   NES-NIR-GECO1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. FSW-HRP-V5-LRRTM1

   Plasmid

   #82536

   Purpose

   Lentiviral vector for expressing HRP-LRRTM1 in neurons

   Depositor

   Alice Ting

   Article

   Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

   Insert

   LRRTM1 (LRRTM1 Human)
   

   Use

   Lentiviral

   Tags

   HRP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Human Synapsin

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Dnase1L3 R206C pIRES-DsRed

   Plasmid

   #98847

   Purpose

   Expresses Human Dnase1L3 with activity-reducing HUVS mutation R206C in mammalian cells along with DsRed

   Depositor

   Peter Keyel

   Article

   Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

   Insert

   Dnase1L3 (DNASE1L3 Human)
   

   Use

   Tags

   IRES DsRed

   Expression

   Mammalian

   Mutation

   R206C which greatly reduces nuclease activity

   Promoter

   CMV

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. hCCR5-mTFP1

   Plasmid

   #110193

   Purpose

   Encodes for human CCR5 coding sequence tagged with the mTFP1 FP

   Depositor

   Sergi Padilla Parra

   Article

   Iliopoulou et al Nat Struct Mol Biol. 2018 Sep;25(9):814-

   Insert

   hCCR5 (CCR5 Human)
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FLAG-hIes2

   Plasmid

   #15355

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   PAP-1 binding protein (INO80B Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pInducer20 TIFA WT

   Plasmid

   #231589

   Purpose

   TIFA WT gene under control of a doxycycline-inducible promoter

   Depositor

   Thomas Henry

   Article

   Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

   Insert

   TIFA (TIFA Human)
   

   Use

   Lentiviral

   Tags

   3X flag

   Expression

   Mammalian

   Mutation

   Promoter

   doxycycline

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-delta133HtrA-EGFP

   Plasmid

   #14124

   Depositor

   L. Miguel Martins

   Article

   Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

   Insert

   HtrA2 (HTRA2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of the first 133 amino-acids

   Promoter

   Available since

   March 7, 2007

   Availability

   Academic Institutions and Nonprofits only

9. C terminal Flag Mios pRK5

   Plasmid

   #46326

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   Mios (MIOS Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR221-FGFR4

    Plasmid

    #60531

    Purpose

    Entry vector for human FGFR4

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    FGFR4 (FGFR4 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    c2134a P712T

    Promoter

    NA

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pEMS1988

    Plasmid

    #49119

    Purpose

    AAV plasmid with CAGGS promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-CAGGS-iCre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. sh-SRF

    Plasmid

    #100797

    Purpose

    expression of shRNA targeting SRF

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Dis. 2015 Oct 29;6:e1944. doi

    Insert

    rat SRF
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Flag WDR24 pLJM1

    Plasmid

    #46337

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Lentiviral

    Tags

    FLag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

14. ATG-1929

    Plasmid

    #108712

    Purpose

    CBR2opt in pF4Ag expression vector with T7 and CMV promoter

    Depositor

    Keith Wood

    Article

    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

    Insert

    CBR2opt
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promega used directed evolution to create CBR (Cl…

    Promoter

    T7, CMV

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL410-BRN2p

    Plasmid

    #110733

    Purpose

    Luciferase reporter for the human BRN2 promoter

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    BRN2 promoter luciferase (POU3F2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGAPTrap TALEN 2

    Plasmid

    #83369

    Purpose

    Partner to pGAPTrap-TALEN1, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    GAPDH_TALEN2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pGAPTrap-TALEN1

    Plasmid

    #83368

    Purpose

    Partner to pGAPTrap-TALEN2, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    GAPDH_TALEN1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pAX198

    Plasmid

    #173042

    Purpose

    Custom vector for sgRNA library construction

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Insert

    HBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AXL KD

    Plasmid

    #105934

    Purpose

    Mammalian expression of myc-his tagged AXL kinase dead (K558R) mutant

    Depositor

    Rosa Marina Melillo

    Article

    Avilla et al Cancer Res. 2011 Mar 1;71(5):1792-804. d

    Insert

    AXL (AXL Human)
    

    Use

    Tags

    His and myc

    Expression

    Mammalian

    Mutation

    K558R, kinase dead

    Promoter

    tet-inducible CMV

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cterminal HA Mios pRK5

    Plasmid

    #46329

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Mios (MIOS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pEMS2021

    Plasmid

    #49128

    Purpose

    AAV plasmid with DCX (Ple302) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple302-iCre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    DCX

    Available since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. H2B-mAvicFP1

    Plasmid

    #129511

    Purpose

    Expresses H2B-mAvicFP1 in mammalian cells (human histone 2B)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    H2B-mAvicFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mAvicFP1 fused to the C terminus of human histone…

    Promoter

    CMV

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. JE301: pMVP (L5-L4) Tir1-P2A-AID

    Plasmid

    #121722

    Purpose

    pMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    osTir1-P2A-AID (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    9x myc (C-term on osTIR1)

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA

    Plasmid

    #47458

    Purpose

    LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE (Neurog2)-GS-CIB1(NLS*,∆318-334)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV_hSyn1_AchLightR

    Plasmid

    #187463

    Purpose

    Expresses red acetylcholine indicator AchLightR in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    AchLightR
    

    Use

    AAV

    Tags

    Igκ leader

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENTR1A mKate2-hu_ncOGT K842M

    Plasmid

    #154284

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    K842M mutation (Catalytic Inactive)

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG-Venus-MDGA1

    Plasmid

    #82543

    Purpose

    Overexpression vector for Venus-MDGA1 in neurons

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    MDGA1 (MDGA1 Human)
    

    Use

    Tags

    Venus fluorescent protein

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta actin

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. J-sRNA-331Bb

    Plasmid

    #78285

    Purpose

    An inducible small RNA with E.coli Hfq targeting K. rhaeticus UGPase mRNA

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    j-sRNA-331
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNCST-mAvicFP1

    Plasmid

    #129509

    Purpose

    Expresses mAvicFP1 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    mAvicFP1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p3E-EEA1

    Plasmid

    #109560

    Purpose

    Multisite gateway vector for 5' tagging with early endosome associated 1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    early endosome associated 1 (eea1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. myc-Atg14 (71-181)

    Plasmid

    #86753

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 71-181

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLCKO_LacZ_sgRNA

    Plasmid

    #74179

    Purpose

    lentiviral vector expressing sgRNA targeting LacZ

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    LacZ sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. myc-Atg14 (1-181)

    Plasmid

    #86752

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 1-181

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBF_HsPepT1

    Plasmid

    #73943

    Purpose

    Xenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tag

    Depositor

    Simon Newstead

    Article

    Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

    Insert

    PepT1 (SLC15A1 Human)
    

    Use

    Make rna for xenopus oocyte injection

    Tags

    Flag

    Expression

    Mutation

    Promoter

    beta globin

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

35. pCAG-Venus-MDGA2

    Plasmid

    #82544

    Purpose

    Overexpression vector for Venus-MDGA2 in neurons

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    MDGA2 (Mdga2 Rat)
    

    Use

    Tags

    Venus fluorescent protein

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta actin

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBF_HsPepT2

    Plasmid

    #73944

    Purpose

    Xenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tag

    Depositor

    Simon Newstead

    Article

    Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

    Insert

    PepT2 (SLC15A2 Human)
    

    Use

    Make rna for xenopus oocyte injection

    Tags

    Flag

    Expression

    Mutation

    Promoter

    beta globin

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

37. pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

    Plasmid

    #99229

    Purpose

    Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos2
    

    Use

    Tags

    Lifeact

    Expression

    Mammalian

    Mutation

    A69T for primed conversion applications

    Promoter

    CMV

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. myc-Atg14 (1-200)

    Plasmid

    #86750

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    Atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 1-200

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. actc1b-mKate2-CDC42

    Plasmid

    #109531

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to CDC42

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    CDC42 (cdc42 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEMS1943

    Plasmid

    #82565

    Purpose

    High copy number plasmid containing Ple266 (S100B MiniPromoter) insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple266
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pEMS1997

    Plasmid

    #49127

    Purpose

    AAV plasmid with SLC6A4 (Ple198) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple198-iCre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SLC6A4

    Available since

    May 7, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. actc1b-mKate2-CDC42-DN

    Plasmid

    #109532

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to dominant negative CDC42

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    CDC42 (cdc42 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    DN

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. scADGFP

    Plasmid

    #114434

    Purpose

    Epilepsy promoter enhanced with Arc elements driving GFP with SV40E polyA

    Depositor

    Edward Perez-Reyes

    Article

    Burke et al Int J Mol Sci. 2023 Sep 23;24(19):14467.

    Insert

    Green fluorescent protein
    

    Use

    AAV

    Tags

    ER export sequence FCYENEV

    Expression

    Mammalian

    Mutation

    Promoter

    Novel synthetic activity-dependent promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pEMS2143

    Plasmid

    #104356

    Purpose

    AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-smCBA-EmGFP-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    smCBA

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pOPINK-OTUD4 (OTU, aa 22-196)

    Plasmid

    #61413

    Purpose

    Expresses human OTUD4 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-21 and aa 197-1114.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pTarget-RFP-GFP operon

    Plasmid

    #192279

    Purpose

    RFP GFP fusion protein expressed under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacIq

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. SpyCas9 PE2*-3HA

    Plasmid

    #169854

    Purpose

    Mammalian Expression, SpyCas9 PE2* prime editor with 3HA-tag

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SpCas9-H840A-star-M-MLV-3HA
    

    Use

    Tags

    3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pENTR1A mKate2-hu_ncOGT D554N

    Plasmid

    #154283

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    D554N mutation (Ser/Thr Glycosylation Impaired)

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pEMS2031

    Plasmid

    #49134

    Purpose

    AAV plasmid with OLIG1 (Ple305) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple305-iCre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    OLIG1

    Available since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

50. LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid

    #47455

    Purpose

    LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    NLS(alpha-imp)-CRY2PHR-NLS-VP64
    

    Use

    AAV

    Tags

    2A_GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge