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169,450 results

Showing: 4901 - 4950 of 169450 results

  2. TRUPATH Triple GaoA

    Plasmid
    #196051
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196051-plasmid-map-sequence-id-385865

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  9. mEmerald-Integrin-Beta1-N-18

    Plasmid
    #54129
    Purpose
    Localization: Integrin/Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Integrin-Beta1 (ITGB1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54129-plasmid-map-sequence-id-314085

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  14. pCINEO-3xFlag-hRIPK3

    Plasmid
    #112495
    Purpose
    Mammalian expression of 3xFlag-hRIPK3
    Depositor
    Junying Yuan
    Insert
    RIPK3 (RIPK3 Human)
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112495-plasmid-map-sequence-id-214535

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  18. pALOD4

    Plasmid
    #111026
    Purpose
    Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A
    Depositor
    Arun Radhakrishnan
    Article
    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d
    Insert
    Domain 4 of Anthrolysin O
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Mutations: S404C, C472A
    Promoter
    T7
    Available Since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111026-plasmid-map-sequence-id-214044

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  20. scADGFP

    Plasmid
    #114434
    Purpose
    Epilepsy promoter enhanced with Arc elements driving GFP with SV40E polyA
    Depositor
    Edward Perez-Reyes
    Article
    Burke et al Int J Mol Sci. 2023 Sep 23;24(19):14467.
    Insert
    Green fluorescent protein
    Use
    AAV
    Tags
    ER export sequence FCYENEV
    Expression
    Mammalian
    Promoter
    Novel synthetic activity-dependent promoter
    Available Since
    Sept. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    114434-plasmid-map-sequence-id-225661

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  26. pBP-T7_RBS-His6-Thrombin

    Plasmid
    #72979
    Purpose
    T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    T7+RBS+His6+thrombin
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  34. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (AAV Retrograde)

    Viral Prep
    #154868-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (#154868). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ulrik Gether
    Promoter
    Human Synapsin-1
    Tags
    mCherry (Flp-dependent)
    Available Since
    Oct. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  35. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV1)

    Viral Prep
    #104492-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available Since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  38. pJAB988

    Plasmid
    #133920
    Purpose
    miniICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoter
    Depositor
    Christopher Voigt
    Article
    Brophy et al Nat Microbiol. 2018 Sep;3(9):1043-1053.
    Insert
    miniICE-tet(M)cat(PC194)_PT7-GFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    133920-plasmid-map-sequence-id-409802

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  42. pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 (AAV1)

    Viral Prep
    #100846-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 (#100846). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    CAG
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only

  50. eIF2a 1

    Plasmid
    #21807
    Depositor
    David Ron
    Insert
    eIF2a (EIF2S1 Human)
    Expression
    Mammalian
    Available Since
    Sept. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    21807-plasmid-map-sequence-id-351672

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Showing: 4901 - 4950 of 169450 results