161,697 results

1. pCMV4-ApoE2

   Plasmid

   #87085

   Purpose

   Expresses ApoE2 in Mammalian cells

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   Apolipoprotein E2 (APOE Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTK_Pax7_enh_195_Citrine

   Plasmid

   #130604

   Purpose

   enhancer reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   Pax7_enh_195
   

   Use

   Enhancer reporter

   Tags

   Expression

   Mutation

   Promoter

   thymidine kinase minimal promoter

   Available since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMSCV-hygro-STING

   Plasmid

   #102598

   Purpose

   Express STING for IFN-beta luciferase reporter assay.

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJIT163-AFID-3

   Plasmid

   #167359

   Purpose

   For inducing predictable deletions in protoplasts and wheat plants

   Depositor

   Caixia Gao

   Article

   Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

   Insert

   A3A-Cas9-UDG-T2A-AP lyase
   

   Use

   Tags

   Expression

   Plant

   Mutation

   WT

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-28c S2

   Plasmid

   #171920

   Purpose

   Express RNA-based (p)ppGpp sensor

   Depositor

   Mingxu You

   Article

   Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

   Insert

   RNA-based (p)ppGpp sensor
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AiP13563 - pAAV-AiE0170h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3563)

   Plasmid

   #224029

   Purpose

   AiE0170h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Sst+ neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   minBG

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG8Ha-EWS-FLI1_Type2

   Plasmid

   #188042

   Purpose

   Expresses His8-EWS-FLI1(TypeII) with a TEV site for removal of the His8 tag.

   Depositor

   David Libich

   Article

   Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

   Insert

   EWS-FLI1 (EWSR1 Human)
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBIG1e-CDK12/Cyclin K

   Plasmid

   #231730

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Inserts

   CDK12 (CDK12 Human)
   CCNK (CCNK Human)
   

   Use

   Tags

   Flag-3C

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBIG1e-CDK13/Cyclin K

   Plasmid

   #231731

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Inserts

   CDK13 (CDK13 Human)
   CCNK (CCNK Human)
   

   Use

   Tags

   Flag-3C

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBMN-MEF2C

    Plasmid

    #232627

    Purpose

    Lentiviral plasmid expressing mouse MEF2C.

    Depositor

    Claes Wadelius

    Article

    Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

    Insert

    MEF2C (Mef2c Mouse)
    

    Use

    Lentiviral

    Tags

    HA-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-HA-RAD52-∆C

    Plasmid

    #235677

    Purpose

    Expresses HA-tagged mutant human RAD52 in mammalian cells with a C-terminal deletion (Δ302-410 amino acids).

    Depositor

    Simon Powell

    Article

    Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

    Insert

    RAD52 (RAD52 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 302-410

    Promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa

    Plasmid

    #233035

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter

    Depositor

    Jonathan Ploski

    Insert

    RfxCas13d-compatible gRNA expression cassette and CasRx
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pXJ-His-PDCD1LG2

    Plasmid

    #232660

    Purpose

    transient expresses PD-L2 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    PDCD1LG2 (PDCD1LG2 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AiP12311 - pAAV-AiE0226h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2311)

    Plasmid

    #230108

    Purpose

    AiE0226h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS2+ GFP-2xESP3I-IRES-mCherry

    Plasmid

    #230996

    Purpose

    Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Type

    Empty backbone

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAV-CAG-oScarlet-WPRE-BC5-pA

    Plasmid

    #220769

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al bioRxiv 2024.04.24.590975

    Insert

    oScarlert-WPRE-BC5
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAV-CAG-oScarlet-WPRE-BC4-pA

    Plasmid

    #220768

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al bioRxiv 2024.04.24.590975

    Insert

    oScarlert-WPRE-BC4
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-memRFP-3xControlgRNA

    Plasmid

    #224567

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    mRFP1
    

    Use

    CRISPR

    Tags

    Membrane localization signal

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(Q63L)-FKBP-mVenus-CAAX

    Plasmid

    #214282

    Purpose

    Express split-RhoA fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
    ras homolog family member A (RHOA Human)
    

    Use

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    RhoA-Q63L

    Promoter

    CMV

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAV-CAG-oScarlet-WPRE-BC6-pA

    Plasmid

    #220770

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al bioRxiv 2024.04.24.590975

    Insert

    oScarlert-WPRE-BC6
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAV-CAG-oScarlet-WPRE-BC3-pA

    Plasmid

    #220767

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al bioRxiv 2024.04.24.590975

    Insert

    oScarlert-WPRE-BC3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p21-HA(RxL)-NeoR

    Plasmid

    #215106

    Purpose

    Expresses p21-HA(RxL) in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    p21(RxL) denotes mutations: R19A, R20A, L21A, F22…

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEM112B

    Plasmid

    #210602

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Ater-mClover3-SpH2Apro | SpH2Bpro-GP2A-GT2A-Hshph-Synter8
    

    Use

    Tags

    mClover3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpH2A/Bpro (divergent)

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1(+)Gαi3-91-NLuc

    Plasmid

    #207150

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAI3 (GNAI3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)K

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AiP1347 - pAAV-AiE2E118m-minBGpromoter-SYFP2-WPRE3-bGHpA

    Plasmid

    #208136

    Purpose

    Direct-expressing SYFP2 in astrocytes AAV Virus. Alias: AiP1347 - pAAV-AiE2118m-minBG-SYFP2-WPRE-BGHpA

    Depositor

    The Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Mich et al bioRxiv 2023.09.20.558718

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBabepuro-TGFA

    Plasmid

    #189610

    Purpose

    Retroviral expression vector for TGFA

    Depositor

    Thierry Tchenio

    Insert

    TGFA (TGFA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    SV40 early promoter

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Diffusive 54kDa

    Plasmid

    #201337

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    EGFP-4PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Diffusive 47kDa

    Plasmid

    #201336

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    EGFP-3PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TFORF1364

    Plasmid

    #142381

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ELOF1 (ELOF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA)

    Plasmid

    #195552

    Purpose

    Expresses Thbs1 under control of short GFAP promoter

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    short GFAP

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. H1.99.luc

    Plasmid

    #193663

    Purpose

    Expresses a truncated Polymerase III H1 promoter of 99 nucleotides (H1-99)

    Depositor

    Elena Herrera-Carrillo

    Article

    Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

    Insert

    Truncated Pol III H1 promoter (H1.99) + 41 nucleotides
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AatII-pRPS5A-PTP-3Xflag-16S rRNA Right_G1397C_ABE

    Plasmid

    #189643

    Purpose

    16S rRNA Right_G1397C_ABE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

    Insert

    TALE and DddAtox and TadA 8e
    

    Use

    TALEN

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRPS5A-PTP-3Xflag-16S rRNA Left_G1397N

    Plasmid

    #189641

    Purpose

    16S rRNA Left_G1397N

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

    Insert

    TALE and DddAtox
    

    Use

    TALEN

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pACE-GIRK4-mC-S

    Plasmid

    #172427

    Purpose

    Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK4 (KCNJ5 Human)
    

    Use

    Tags

    mCherry Streptag II

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pIG-747pUC19-tNGFR-P2A-CD7

    Plasmid

    #186086

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD7 (CD7 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mt-DdCBE G1333-C

    Plasmid

    #174561

    Purpose

    mitochondrial-targeting DdCBE (split DddA, G1333-C) Golden Gate destination vector

    Depositor

    Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

    Insert

    DdCBE G1333-C
    

    Use

    Tags

    FLAG

    Expression

    Plant

    Mutation

    Promoter

    PcUbi

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mt-DdCBE G1333-N

    Plasmid

    #174560

    Purpose

    mitochondrial-targeting DdCBE (split DddA, G1333-N) Golden Gate destination vector

    Depositor

    Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

    Insert

    DdCBE G1333-N
    

    Use

    Tags

    HA

    Expression

    Plant

    Mutation

    Promoter

    PcUbi

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTK_TFAP2b_enh_226_Citrine

    Plasmid

    #130618

    Purpose

    enhancer reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    TFAP2b_enh_226
    

    Use

    Enhancer reporter

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. ESAM-bio-His

    Plasmid

    #51751

    Purpose

    Expresses full-length Endothelial cell-selective adhesion molecule precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ESAM (ESAM Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-CaMKII-PinkyCaMP

    Plasmid

    #232858

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CaMKII promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBiex-1 GFPNanobody_SNAP

    Plasmid

    #82711

    Purpose

    Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

    Insert

    GFP Nanobody
    

    Use

    Tags

    6xHis Purification tag, FLAG purification tag, an…

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    T7 Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. AAV-CAG-GFP

    Plasmid

    #28014

    Depositor

    Karel Svoboda

    Article

    Mao et al Neuron. 2011 Oct 6;72(1):111-23.

    Insert

    eGFP
    

    Use

    AAV; Adeno associate viral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

    Plasmid

    #170186

    Purpose

    This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

    Depositor

    Bradley Bernstein

    Article

    Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

    Insert

    CreERT2-T2A-eBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pBAD-I-SceI

    Plasmid

    #60960

    Purpose

    Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

    Depositor

    Nikolaus Osterrieder

    Article

    Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

    Insert

    I-SceI restriction enzyme
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. AbVec1.1-IGLC2-XhoI

    Plasmid

    #99575

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid

    #58477

    Purpose

    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed Arginine 62 to Aspartic Acid

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. JunB

    Plasmid

    #187903

    Purpose

    Expresses JunB in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    JunB (Junb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. p2-39

    Plasmid

    #118086

    Purpose

    ZB-linker-split C-terminal T7 RNAP expression plasmid

    Depositor

    Bryan Dickinson

    Article

    Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

    Insert

    ZB-linker-split C-terminal T7 RNAP expression plasmid
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pSBbi G418R mbIL21

    Plasmid

    #194329

    Purpose

    Consitiutive mammalian expression of membrane bound human IL-21

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Insert

    mbIL-21 (IL21 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pET-NLS-Cas9-6xHis

    Plasmid

    #62934

    Purpose

    Expression of NLS-Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    NLS-Cas9-6xHis
    

    Use

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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