168,189 results
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qTAG-C-mStayGold-EFS-Blast
Plasmid#227257PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Blast cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5/DEST-VPS35
Plasmid#21691DepositorInsertVacuolar Protein Sorting 35 homolog (VPS35 Human)
UseLentiviral; Invitrogen gateway destination vectorTagsV5ExpressionMammalianAvailable SinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP1
Plasmid#129499PurposeExpresses AausFP1 constitutively in E. coli (most strains)DepositorInsertAausFP1
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q30
Plasmid#111744PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ30 (polyQ repeat)PromoterCMV and P10Available SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Myc DBC1
Plasmid#35096DepositorAvailable SinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC30A8
Plasmid#132136PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC30A8 (SLC30A8 Human)
ExpressionMammalianAvailable SinceNov. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPROEX Aqua
Plasmid#42889DepositorInsertAquamarine
Tags6xHis and TEV cleavageExpressionBacterialMutationT65S and H148GAvailable SinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Fis1
Plasmid#182580PurposeExpresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequenceDepositorInsertmCherry-Fis1
TagsFis1ExpressionMammalianAvailable SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
FRE5
Plasmid#62377PurposeFluorescent reporter to quantify the ratio of two alternative splicing patterns.DepositorInsertdsRed and GFP
ExpressionMammalianAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB-gDA3m-IRES-mCherry-CAAX
Plasmid#208692PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m and a membrane-localized mcherry in mammalian cellsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_gDA3m
ExpressionMammalianPromoterCMVAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP
Plasmid#216479Purposelentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excisionDepositorAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11
Plasmid#162037PurposeBackground-free mRNA labeling tripartite superfolder GFP systemDepositorInsertGFP1-9 P2A MCP-GFP10 P2A PCP-GFP11
UseLentiviralExpressionMammalianAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SaPE2
Plasmid#174817PurposeMammalian expression of SaCas9 prime editor 2DepositorInsertSaPE2
TagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9-Venus
Plasmid#70267PurposeExpresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.DepositorInsertsCas9
Venus Reporter
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEFS-NS and P2AAvailable SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19 Giles integrase
Plasmid#170049PurposepUC19 with Giles integraseDepositorInsertGIles Integrase
ExpressionBacterialAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G
Plasmid#177960PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)DepositorInsertSARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
ExpressionMammalianMutationD614GAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTNR1B-DuET
Plasmid#213349PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Luciferase
Plasmid#213979PurposeExpresses Doxy Inducible LuciferaseDepositorInsertLuciferase
UseLentiviralTags6xHis affinity tagExpressionBacterial and MammalianPromoterTet-responsive promoter PTight, consisting of sev…Available SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mTagBFP2
Plasmid#99267PurposeContains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertmTagBFP2-3XHA
TagsH2BExpressionMammalianPromoterSP6Available SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 BRGI-Flag
Plasmid#19143DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
TagsFlagExpressionMammalianAvailable SinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-GFPd2
Plasmid#115665PurposePiggybac transposon plasmid for destabilized eGFP (GFPd2)DepositorInsertGFPd2
UseSynthetic BiologyTagsDestabilizationExpressionMammalianPromoterCAGAvailable SinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-CRBN-P2A-Hygro
Plasmid#124303PurposeLentiviral vector for expression of Flag tagged CRBN-P2A-Hygro casette from a CMV promoterDepositorAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRU42
Plasmid#167664PurposeENTRY vector for gRNA2 cloning under pAtU3 promoterDepositorInsertpAtU3
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT120a17
Plasmid#138510PurposeP1DepositorInsertgIII-xLuxAB
UseSynthetic BiologyAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NR2A
Plasmid#17924DepositorAvailable SinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRL_GI_PCB28
Plasmid#98745PurposeFor phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.DepositorInsertsUseSynthetic BiologyExpressionYeastMutationCodon optimized for Saccharomyces cerevisiaePromoterADH1m and PGK1Available SinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET-4xTR-TUBE
Plasmid#110312PurposeE. coli expression and purification of 4xTR-TUBEDepositorInsert4xTR-TUBE (UBQLN1 Human)
TagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.GFP
Plasmid#57827PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP
UseCRISPR and LentiviralTagsFLAGAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xFLAG-Pontin
Plasmid#51635PurposeMammalian expression of 3xFLAG tagged pontin, N-terminal.DepositorAvailable SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only
