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pQCasTns(Ptr)-array 8
Plasmid#175580PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)
UseTagsExpressionBacterialMutationNOPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDUET-1-alpha-synuclein-mCerulean3-His6
Plasmid#110061PurposeFor bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidinesDepositorInsertalpha-synuclein (SNCA Human)
UseTags6 x His and mCerulean3ExpressionBacterialMutationPromoterT7Available sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) NRP1 b1 (273-427)
Plasmid#162734PurposeBacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.DepositorInsertHuman NRP1 b1 domain residues 273-427
UseTagsExpressionBacterialMutationCodon optimisedPromoterAvailable sinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFNR5
Plasmid#149483PurposeBroad-Host range expression vector, RSF1010 origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertNahR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPSalTTCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATmin-GG
Plasmid#191352PurposeClostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.419
Plasmid#187208PurposeExpresses wild-type retron Eco4 ncRNA, and Cas1 + Cas2DepositorInsertsRetron Eco4 ncRNA
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7/LacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
GSK3beta/pET29b
Plasmid#16315DepositorInsertGSK3beta (gsk3b.L Frog)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA
Plasmid#105000PurposeExpresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorInsertRenilla luciferase
UseTags24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …ExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-ELP[V-60]
Plasmid#67013PurposeExpresses malE-ELP[V-60] in E. coliDepositorInsertmalE-ELP[V-60]
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKD182
Plasmid#90952PurposeExpresses ThsS under the Ptac promoterDepositorInsertThsS
UseTagsExpressionBacterialMutationPromoterPtacAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
5KO9 (HMCES)
Plasmid#101877PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHMCES (HMCES Human)
UseTags6x His (modified C-term tag is AENLYFQSHHHHHH)ExpressionBacterialMutationPromoterT7Available sinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTriEx-mVenus-PA-Rac1
Plasmid#22007PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
UseTags6X His and mVenusExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546), Rac1 starts at I4 and contain…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAD12
Plasmid#34832DepositorInsertNone
UseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp3 C163A-His
Plasmid#11822DepositorInsertCaspase 3 catalytic mutant (CASP3 Human)
UseTags6xHisExpressionBacterialMutationC163APromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-SpEndoH
Plasmid#218196PurposeBacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoHDepositorInsertEndoH
UseTagstufA-His-CBDExpressionBacterialMutationcodon optimized Ecoli, residues 45-313PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)
Plasmid#181791PurposeExpresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrcECT
Plasmid#75477PurposeInducible expression of ectABC in E. coliDepositorInsertectABC
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMGB
Plasmid#139932PurposeM. magneticum/E. coli shuttle vector (partial sequence of pMGT and p15A)DepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23101Available sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOSV00170
Plasmid#222934PurposeConstitutive GFP expression for Bacillus subtilisDepositorInsertGFP(Sp)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPhyperspankAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-SARS-CoV-2-Nucleocapsid
Plasmid#204403PurposeE. coli expression of full-length SARS-CoV-2 nucleocapsid with an N-terminal mCherry tagDepositorInsertSARS-CoV-2 nucleocapsid (N SARS-CoV-2)
UseTags6x-His; mCherryExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rb-379-792-pGex2TK
Plasmid#21748DepositorInsertRb (RB1 Human)
UseTagsGSTExpressionBacterialMutationdeletion mutant ORF contains aa 379-792 of wt Rb …PromoterAvailable sinceAug. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pPEPZ-Plac
Plasmid#122635PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEST-OR2-OR1-Pr-UTR1-deGFP-T500
Plasmid#40019DepositorInsertdeGFP
UseTagsExpressionBacterialMutationPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceOct. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQF
Plasmid#48095PurposeCumate-inducible expression of 3xFLAG-tagged fusion proteinDepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterPQ5Available sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAS1yl
Plasmid#73226PurposeConstitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cellsDepositorInsertsCas9
sgRNA
UseCRISPR; In y. lipolyticaTagsExpressionBacterialMutationPromoterunknownAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE-60NA-proinsulin-mEGFP
Plasmid#160464PurposeEncodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteriaDepositorInsertproinsulin-mEGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2T-CAG-SpCas9-BlastR
Plasmid#107190PurposeConfers constitutive expression of SpCas9DepositorInsertSpCas9 (NEWENTRY )
UseCRISPRTags3xFLAGExpressionBacterial and MammalianMutationPromoterChicken ß-actinAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-BCA-Q11
Plasmid#185781PurposeEncodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-Q11
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB2Hopt
Plasmid#163830PurposeTwo-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1BDepositorInsertSpecR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPro1;ProD;pLacZ-OptAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJF143.J3
Plasmid#153028PurposeJ3-mRFP1DepositorInsertJ3-mRFP1
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINS-UBE3C
Plasmid#66711PurposeExpresses UBE3C HECT domain in E. coliDepositorInsertKiAA0010 (UBE3C )
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Human Parkin (1-465)
Plasmid#110758PurposeBacterial expression of human ParkinDepositorInsertPRKN (PRKN Human)
UseTagsN-His6-GST-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAT202
Plasmid#134879PurposeBacterial expressionDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationdeletion of Y208PromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pTA-Mob 2.0 Tp
Plasmid#188619PurposeMutated pTA-Mob 2.0 Backbone in TraJ Promoter RegionDepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHCL149
Plasmid#134457PurposeCmR, Para-popZ-rbs-H3H4-msfGFPN-CTM. Replicative plasmid for the expression of bait. N-terminal GFP fusion.DepositorInsertPara-popZ-rbs-H3H4-msfGFPN-CTM.
UseTagsExpressionBacterialMutationPromoterParaAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM597
Plasmid#68886PurposeIPTG-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertsNanoLuc
LacI
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBL715
Plasmid#140385PurposeExpresses MphR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-MphR-T
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_A321V
Plasmid#98671PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with A321VDepositorInsertTDP-43 (TARDBP Human)
UseTags6xHisExpressionBacterialMutationA321VPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only
