31,059 results
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Plasmid#8608DepositorInsertbarstar
ExpressionBacterialAvailable SinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSEVA421-Cas9tr
Plasmid#138709PurposePlasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenesDepositorInserttracrRNA-Cas9
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
TwinStrep-SUMO-NLS-BhCas12b_v4
Plasmid#158615PurposeFor protein expression of BhCas12b_v4DepositorInsertNLS-BhCas12b_v4
Tags6xHIS-TwinStrep-SUMOExpressionBacterialAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST-2K
Plasmid#44564Purposefor ectopic constitutive expression of two mycobacterial proteins in one vectorDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB
Plasmid#30505DepositorAvailable SinceNov. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGRG37
Plasmid#16667DepositorInsertmTn7::Gateway cassette
ExpressionBacterialAvailable SinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSR1037
Plasmid#208736PurposeCharacterization plasmid for PSarA1 (sfgfp expressed from PSarA1 on the S. aureus pC194 replicon)DepositorInsertPSarA1
UseSynthetic BiologyExpressionBacterialAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-sumo-h-cGASCD(K427E/K428E)
Plasmid#127162Purposeexpression of truncated human cGAS proteinDepositorAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRedCmOcr
Plasmid#176226PurposeA helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.DepositorInsertLambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyExpressionBacterialPromoterPrhaB and PLAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW-LIC-N8C_Fab3b
Plasmid#203685PurposeExpresses N8C_Fab3bDepositorInsertN8C_Fab3b
ExpressionBacterialAvailable SinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSMrnaSeph
Plasmid#67507PurposeExpress the tRNAMet scaffold with the sephadex aptamer tagDepositorInsertmodified tRNAMet with sephadex aptamer
ExpressionBacterialPromoterlppAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
O-TREM2-2
Plasmid#166545PurposescFv of a human scaffold targeting Triggering receptor expressed on myeloid cells 2. Antigen coverage aa 19-131 of 230DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
poRibo-T2
Plasmid#69347PurposeRibo-T with orthogonal anti-Shine-Dalgarno sequence, and pL G to T mutationDepositorInsertoRibo-T
ExpressionBacterialAvailable SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
TAF1
Plasmid#39117PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy44
Plasmid#55185PurposeInsect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneTags10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…ExpressionBacterialPromoterpPH (Polyhedrin promoter)Available SinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_PCNA_IRES_puro2b
Plasmid#21048DepositorInserthPCNA
TagsGFPExpressionBacterial and MammalianAvailable SinceMay 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PRKACB_Pkinase
Plasmid#110024PurposeProtein expression and purification of PRKACB_PkinaseDepositorInsertPRKACB_Pkinase (PRKACB Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEEK2
Plasmid#83492PurposeExpression plasmid for conjugational transfer into cyanobacteriaDepositorTypeEmpty backboneUseSynthetic BiologyTagsStrep-tagExpressionBacterialPromoterPtrc coreAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQCH
Plasmid#164160PurposeIncQ BHR plasmidDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRECMYC-Ebu1
Plasmid#240713PurposepLAM12 vector backbone containing Ebu1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155DepositorInsertEbu1 RT, Eco1 ncRNA, MspRecT
ExpressionBacterialMutationWTAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJAK184.tetR.PT5-3.dCas9
Plasmid#232482Purposeoptimized ahTet inducible dCas9 for insertion into C. difficile genomeDepositorInsertCatalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
UseCRISPRExpressionBacterialPromoterTetR-PT5-3Available SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJC.15A.sgRNA.TA
Plasmid#232483PurposesgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.DepositorInsertsToxin-antitoxin System from CD630
medium-copy-number p15A origin of replication
mCherry
UseCRISPRExpressionBacterialPromoterP3 constitutive promoter and native promoterAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCW-LIC-N8C_Fab2b
Plasmid#203692PurposeExpresses N8C_Fab2bDepositorInsertN8C_Fab2b
ExpressionBacterialAvailable SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pE5770
Plasmid#170777Purposeexpression of hybrid proteins in E. coliDepositorAvailable SinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCPP3373
Plasmid#128718PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopQ1-1
ExpressionBacterialMutationSynonymous point mutation in the wobble position…Available SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-mCh-FYVE
Plasmid#192289PurposeExpresses GST- and mCherry-tagged HRS FYVE in e ColiDepositorInsertHRS FYVE domain
TagsGST-TEV-mCherryExpressionBacterialPromotertacAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFCiKm7
Plasmid#167521PurposeBroad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, KmR, Erythromycin Resistance Marker.DepositorInsertCinRAM-KmR
UseSynthetic BiologyExpressionBacterialPromoterPCinAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIV296
Plasmid#204687PurposeAMA1 plasmid with nat1 resistance markerDepositorInsertnat1
UseFungal expressionExpressionBacterialPromoterAspergillus nidulans trpC promoterAvailable SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
GEF-H1 FLARE212.pa
Plasmid#134930PurposeGEF-H1 biosensor that does not have GEF activity.DepositorInsertGEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
TagsNone.ExpressionBacterial and MammalianPromoterCMV and T7Available SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG163
Plasmid#124365PurposeIPTG inducible system (gfpmut2 with Ppsank) for expression in B. subtilisDepositorAvailable SinceJuly 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80L Hu IL-15-His tag
Plasmid#183256PurposeBacterial expression of human IL-15 with N-terminal His tagDepositorAvailable SinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-dddAI
Plasmid#186559PurposeBroad host range regulated expression of the DddA immunity determinant (DddAI)DepositorInsertdddAI
ExpressionBacterialPromotertacAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKL001
Plasmid#98917PurposeConstitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.DepositorInsertCaPR
ExpressionBacterialPromoterBBa_J23118 - Strong ConstitutiveAvailable SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pFVK5
Plasmid#167522PurposeBroad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, mKelly2, Gentamicin Resistance Marker.DepositorInsertVanRAM-mKelly2
UseSynthetic BiologyExpressionBacterialPromoterPVanCCAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CPDSalI
Plasmid#38252DepositorTypeEmpty backboneTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialPromoterT7Available SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCas1+2
Plasmid#72676PurposeExpress Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.DepositorInsertCas1, Cas2
ExpressionBacterialAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP WT
Plasmid#197575PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replicationDepositorInsertssfGFP WT
M. alvus Pyl-tRNA (6)
TagsHis6ExpressionBacterialPromoteraraC and lppAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLG166
Plasmid#124366PurposeGfpmut2 constitutively expressed using the Ppen promoter for expression in B. subtilisDepositorAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST HP1 beta
Plasmid#24075DepositorAvailable SinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only