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Bacterial Expression

29,744 results

Showing: 6001 - 6050 of 29744 results

  6. AURKA_HUMAN_D0

    Plasmid
    #79739
    Purpose
    This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    Nicholas Levinson, John Chodera, Markus Seeliger
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    AURKA (AURKA Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. p007ampGFP

    Plasmid
    #58535
    Purpose
    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    Green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T5 between two lac operators
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58535-plasmid-map-sequence-id-90574

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  9. IBMc396

    Plasmid
    #161954
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    161954-plasmid-map-sequence-id-328230

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  10. pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)

    Plasmid
    #128165
    Purpose
    UV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).
    Depositor
    Marcus Noyes
    Article
    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928
    Insert
    10 amino acid glycine/serine linker + 5alanines
    Use
    Synthetic Biology
    Tags
    FLAG and zif268
    Expression
    Bacterial
    Mutation
    Promoter
    UV5
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    128165-plasmid-map-sequence-id-267573

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  19. pEVOL-AcKRS-CloDF

    Plasmid
    #127415
    Purpose
    Mutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophage
    Depositor
    Wenshe Liu
    Article
    Wang et al Angew Chem Int Ed Engl. 2019 Oct 28;58(4
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    April 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    127415-plasmid-map-sequence-id-250212

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  21. pBT3000

    Plasmid
    #122536
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (tram-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::tram-1 (tram-1 Synthetic, Nematode)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122536-plasmid-map-sequence-id-238187

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  22. CTHA

    Plasmid
    #42365
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CTHA (CTH Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. pKSJ376

    Plasmid
    #103065
    Purpose
    Expresses colicin E1 with R domain deletion and ImmE1
    Depositor
    Karen Jakes, Alan Finkelstein
    Article
    Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0
    Inserts
    colicin E1 immunity protein (pColE1_26, imm E. coli)
    cea/colicin E1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    R (receptor-binding domain) deletion in colicin E…
    Promoter
    T7 lac (from pET) and T7 lac (from pET52-b)
    Available since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    103065-plasmid-map-sequence-id-200637

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  26. pDM098

    Plasmid
    #216818
    Purpose
    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mStayGold
    Use
    Tags
    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216818-plasmid-map-sequence-id-429609

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  27. pDUP1

    Plasmid
    #223516
    Purpose
    inactive cogfp under control of Ptet, cogfp under control of Ptac
    Depositor
    Yolanda Schaerli
    Article
    Ljiljana et al eLife13:RP97216
    Inserts
    CoGFP
    CoGFP inactivated
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    anti-dimerization mutations S129G, 22 C154S, D156…
    Promoter
    Ptac, Ptac and Ptet, and Ptet
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    223516-plasmid-map-sequence-id-444180

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  34. pFP.R270

    Plasmid
    #138219
    Purpose
    Expresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GGG).IMPDH-1.(SIC)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138219-plasmid-map-sequence-id-273501

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  35. RHOA

    Plasmid
    #73231
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RHOA (RHOA Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00071:Ras 7-180
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73231-plasmid-map-sequence-id-136222

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  39. pUC-GFP-MCC

    Plasmid
    #133307
    Purpose
    "Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation
    Depositor
    Chris Barnes
    Article
    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1
    Insert
    microcin-V bacteriocin cassette (cvaC E. coli)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    N112D (please see depositors comments)
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    133307-plasmid-map-sequence-id-272413

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  41. USP5

    Plasmid
    #25299
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP5.001.835 (USP5 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  45. pNO183

    Plasmid
    #101068
    Purpose
    ppsR2-bphP1 bicistronic operon plasmid
    Depositor
    Jeffrey Tabor
    Article
    Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021
    Inserts
    ppsR2 (ppsR2 Rhodopseudomonas palustris CGA009)
    bphP1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    insert guanine at position 430 to fix frameshift …
    Promoter
    Available since
    Nov. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    101068-plasmid-map-sequence-id-194033

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Showing: 6001 - 6050 of 29744 results