32,656 results
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Plasmid#52734PurposeIPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assayDepositorInsertNGFP+Z-fusion
TagsHis tagExpressionBacterialPromoterT7Available SinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC27
Plasmid#117478Purposemobilizable, SRB-stable, kanamycin-resistant cloning vectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28CLY9
Plasmid#21769DepositorInsertECFP -EYFP linked by flexible linker containing 9 SGGSGG repeats
TagsHisExpressionBacterialAvailable SinceDec. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAI3_G-alpha
Plasmid#110014PurposeProtein expression and purification of GNAI3_G-alphaDepositorInsertGNAI3_G-alpha (GNAI3 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Rb-379-792-pGex2TK
Plasmid#21748DepositorInsertRb (RB1 Human)
TagsGSTExpressionBacterialMutationdeletion mutant ORF contains aa 379-792 of wt Rb …Available SinceAug. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCJR01
Plasmid#187888PurposeEscherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.DepositorInsertDNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
ExpressionBacterialPromoternoneAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24b-SUMO-BG2.4
Plasmid#199438Purposeexpresses BAmGreen2.4 in bacterial cells with a SUMO tagDepositorInsertBAMGreen2.4
Tagshis tag and sumoExpressionBacterialPromoterT7Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CYP51A1 (3I3K)
Plasmid#25161PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCYP51A1_cw_09 (CYP51A1 Human)
TagsHisExpressionBacterialMutationContains amino acid residues 54-502Available SinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
XXBLM (XXBLMA-c075)
Plasmid#98223PurposeVector for periplasmic expression of Lama antibodies with PelA leader and C-terminal His6 tagDepositorAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTlpA_mCherry_YafQ/DinJ
Plasmid#229148PurposeToxin-Antitoxin (Dinj/YafQ)DepositorInsertmCherry, DinJ/YafQ
ExpressionBacterialPromoterPtlpA (mCherry), DinJ/YafQ (Native)Available SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRnc-gGFP161
Plasmid#189536PurposeExpresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFPDepositorInsertsRNase III
gUTR-161-GFP
ExpressionBacterialMutationN/APromoterJ23119 and pBADAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-Caspase-4_enzymatic-domain
Plasmid#183381PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28.His.3C.86b.Tau2N4R
Plasmid#226399PurposeExpresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged WT 2N4R tau for recombinant protein production in bacteriaDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-MerTK-PTPN1
Plasmid#197988PurposeExpression of MerTK Kinase Domain (wild-type) in E. coliDepositorExpressionBacterialPromoterT7 promoterAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM31
Plasmid#135491PurposeAgrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3DepositorInserthph SpunH2A/Bpr hph-mCerulean3
TagsmCerulean3ExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-mDyn2 pcDNA3
Plasmid#36264DepositorAvailable SinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBEST-OR2-OR1-Pr-araC, pBAD-TetR, pBAD-TetO1-deGFP-ssrA
Plasmid#45789DepositorInsertsdeGFP
Tetracycline repressor
araC
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRE133
Plasmid#118487Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsYFP2
ExpressionBacterialAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJBL715
Plasmid#140385PurposeExpresses MphR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-MphR-T
Tags6XHisExpressionBacterialAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-PSmOrange2
Plasmid#34957DepositorInsertPSmOrange2
ExpressionBacterialMutationR17H, R36H, F64I, Q194L, A224S, G226A (mutations …Available SinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) NRP1 b1 (273-427)
Plasmid#162734PurposeBacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.DepositorInsertHuman NRP1 b1 domain residues 273-427
ExpressionBacterialMutationCodon optimisedAvailable SinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET50_MDA5∆2CARD
Plasmid#216804PurposeExpresses melanoma differentiation-associated protein 5 lacking its 2 CARD domainsDepositorInsertmelanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)
ExpressionBacterialMutation∆1-287Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSNRW-Z5-Nluc
Plasmid#174487PurposeRecombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.DepositorInsertZ5-Nluc
Tags6x Histidine tag (N terminal on insert)ExpressionBacterialPromoterT7lac promoterAvailable SinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
CP2-TCC
Plasmid#179679PurposeComplementary plasmidDepositorInsert[sd2] T7 RNAP-TCC linker-degron
ExpressionBacterialAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
6920 pPROEXHsp104b
Plasmid#1230DepositorAvailable SinceAug. 15, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAKlux1
Plasmid#14073DepositorInsertBacterial luciferase
ExpressionBacterialAvailable SinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBSSK WT p85 alpha flag
Plasmid#13429DepositorAvailable SinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_3KB5
Plasmid#234225PurposeHeterologous protein expression of tripartite motif-containing protein 72 (Mg53) in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMT643
Plasmid#8610DepositorInsertbarstar A
ExpressionBacterialMutationTwo cysteines of barstar changed to alanine, C40A…Available SinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pQdC12a
Plasmid#191356PurposeClostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expressionDepositorInsertsPGusA2-TetO2/1-dCas12a (LbCpf1)
miniPthl-tetR
ExpressionBacterialPromoterPGus2A-TetO2/1 and miniPthlAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK094-LM-AMA002.0
Plasmid#171366PurposeEntry Vector compatible, fungal replicating, shuttle backbone vector from the Fungal Modular Cloning ToolKit.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5T6T,P-J23106-geneIII
Plasmid#80863PurposePhagemid (encodes evolved cI4A5T6T,P and M13 gene III)DepositorInsertsM13 gene III
cI4A5T6T,P
ExpressionBacterialPromoterBBa_J23106 and rpoNAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
AK3L1
Plasmid#39135PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSKA413
Plasmid#80381PurposeConstitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.DepositorInsertsccaS (complement)
ccaR (complement)
gfpmut3
UseSynthetic Biology; OptogeneticsTagsFLAG tagExpressionBacterialPromoterccaR and cpcG2Δ59Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-CD11bDARPin
Plasmid#198295PurposeProduces PVCs retargeted with anti-CD11b DARPin F7DepositorInsertPVCpnf_pvc13-CD11bDARPin
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
NUDT16A
Plasmid#42404PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSiMPls_N + pSiMPls_C
Plasmid#167203PurposeSiMPl plasmid pair (pSiMPls) for use with spectinomycin/streptomycinDepositorInsertsEGFP
SmR 1-75 + gp41-1 N-intein
mRuby3
gp41-1 C-intein + SmR 76-263
ExpressionBacterialPromoterAmpR, CAT, pBAD, and pTrcAvailable SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG022 (dsr1)
Plasmid#157900PurposeExpresses the dsr1 defense systemDepositorInsertdsr1 (SIR2-DUF4020)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)
Plasmid#73404PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
PhCHS
CmCHI
ExpressionBacterialPromoterT7Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
TJP1_PDZ_2
Plasmid#103910PurposeProtein expression and purification of ZO1 PDZ2 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2
Plasmid#167849PurposeBacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNADepositorInsertnsp10, nsp16
Tags6xHis-TEVExpressionBacterialPromoterT7Available SinceApril 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRT498-3xOtUBD
Plasmid#190088PurposeExpresses 3 tandem OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsert3xOtUBD
Tags6xHis and MBPExpressionBacterialAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMMLV-mRFP1-P2A-Puro
Plasmid#160228PurposeExpresses mRFP in mammalian cellsDepositorInsertmRFP1-P2A-Puro
UseRetroviralExpressionBacterial and MammalianPromoterLTRAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB061i3
Plasmid#79230PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ; PlacIq lacI
ExpressionBacterialPromoterPpro1; PlacIqAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a-TEX264-deltaLRR-His6
Plasmid#220215PurposeBacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tagDepositorInsertTEX264 (TEX264 Human)
TagsHis6ExpressionBacterialMutationc.4-75del / p.2-24delPromoterT7Available SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAL-rfp_T
Plasmid#74075PurposepAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecRDepositorInsertsgRNA rfp (T)
UseSynthetic BiologyExpressionBacterialPromoterptacAvailable SinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQexp
Plasmid#25820DepositorInsertpQexp
ExpressionBacterialAvailable SinceSept. 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
Spacer Switch Plasmid
Plasmid#215346PurposeFacilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.DepositorInsertOpaD spacer; Chloramphenicol Resistance Cassette
UseCRISPRExpressionBacterialAvailable SinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZE27MC3
Plasmid#75464PurposePlasmid for constitutive expression of mcherry-pdt3DepositorInsertmcherry-pdt#3
ExpressionBacterialPromoterplacIq promoterAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hHBEGF
Plasmid#199234PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProheparin-binding EGF-like growth factor (HBEGF Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only