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Plasmid#197102PurposeTo express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.DepositorInsertTyrRS variant, amber suppressor tRNA
UseTagsExpressionBacterialMutationGly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)PromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
SRF WT pGEX4T
Plasmid#121100PurposeExpression of SRF as a GST fusion proteinDepositorInsertSRF (SRF Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
PARP9-c024
Plasmid#173096PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPARP9 (PARP9 Human)
UseTagsmCeruleanExpressionBacterialMutationG102-K298PromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-624-919
Plasmid#104200PurposeExpresses human AR-624-929 ligand binding domain in pGEX-5X-1 as GST fusion proteinDepositorInserthuman androgen receptor aa 624-919 (AR Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceDec. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESRRA_Hormone-recep
Plasmid#109908PurposeProtein expression and purification of ESRRA_Hormone-recepDepositorInsertESRRA_Hormone-recep (ESRRA Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOSV805
Plasmid#126598Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINN-GFP
Plasmid#53541PurposepTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus systemDepositorInsertGFP
UseTagsN-His-GFPExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBMCS-SortA
Plasmid#21931DepositorInsertsortase (srtA Staphylococcus aureus (strain N315))
UseTagsGB1 and His-tagExpressionBacterialMutationdeleted amino acids 1-59PromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPe_PepTSo
Plasmid#58334PurposeExpresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged proteinDepositorInsertPepTso
UseTagsGFP-HisExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Avitag-SpyCatcher002
Plasmid#102828PurposeBacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylationDepositorInsertAvitag-SpyCatcher002
UseTagsAvitag, GSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK1035
Plasmid#8832DepositorInsertTVMV protease
UseTagsHis and MBP (cleaved)ExpressionBacterialMutationsynthetic gene with codons optimized for E. coliPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-CII
Plasmid#51566PurposeExpression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertcII
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQE-60NA-proinsulin-msGFP2
Plasmid#160466PurposeEncodes a fusion of human proinsulin and the monomeric superfolder GFP2DepositorInsertproinsulin-msGFP2
UseTagsExpressionBacterialMutationPromoterT5Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
K84M kinase-dead Nuak1-3xflag
Plasmid#97219PurposePlasmid expressing kinase-dead mutant Nuak1DepositorInsertNuak1 (NUAK1 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationKinase-dead K84MPromoterT7Available sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTK204
Plasmid#13534DepositorInsertFLIPW-CTYT
UseTagsSee mapExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28b-6His-AcpS
Plasmid#145376PurposeExpression of E. coli AcpSDepositorInsertAcpS (acpP E. coli)
UseTags6-HisExpressionBacterialMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pLAC
Plasmid#45072Purposelacritin-intein fusion plasmid coding for intact mature lacritin without signal peptideDepositorInsertLacritin (LACRT Human)
UseProtein expression vectorTagsInteinExpressionBacterialMutationPromoterT7Available sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTi4.0_SpdCas9_pmcDA1
Plasmid#204613PurposeCRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticumDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMP2834
Plasmid#160673PurposeMobile CRISPRi sfGFP 'test', gmc6 sgRNADepositorInsertgmc6 (gfp) sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mNectarine
Plasmid#21717PurposeBacterial expression of pH-sensitive monomeric mNectarine red fluorescent proteinDepositorInsertmNectarine fluorescent proteins
UseTagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…PromoterAvailable sinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMM660
Plasmid#68889PurposeArabinogalactan-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRF-TetR
Plasmid#49374DepositorInsertTetR repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI
Plasmid#115219PurposeExpresses PspCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPspCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUS212
Plasmid#84580PurposeE.coli cloning and expression vector. Has cloning sites downstream of Tet promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAH121
Plasmid#164901PurposeThe pAH121 plasmid [Haldimann and Wanner 2001] is the phi21 helper plasmid necessary for genomic integration of the pCYMR expression plasmid.DepositorInsertphi21 Integrase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2
Plasmid#196250PurposePlasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119DepositorInsertattL1-GlpT-scGFP-attL2
UseTagsExpressionBacterialMutationPromoterJ23119 promoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
[pSK122] pETDuet GST-p18(delta5)
Plasmid#136144Purposebacterial expression of GST-p18DepositorInsertGST-p18
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJTM-T7-mNeongreen-6xHis
Plasmid#210868PurposePlasmid used for expressing his--tagged mNeongreen for protein purification.DepositorInsertmNeongreen-6xHistag
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKD233.7-3
Plasmid#90955PurposeExpresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutivelyDepositorInsertsTetrathionate response regulator
Superfolder GFP
mCherry
UseTagsExpressionBacterialMutationPromoterBba_J23114, PltetO-1, and PttrB185-269Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR62
Plasmid#125076PurposeExpression of ompR under PLtetO-1, output promoter PompF146DepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET 15b-UbcH5a/2417
Plasmid#11472DepositorInsertUbcH5a (UBE2D1 Human)
UseTagsExpressionBacterialMutationn/aPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-CreiLOV
Plasmid#192817PurposeExpresses CreiLOV in bacteriaDepositorInsertCreiLOV
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
BASIC_3_Kan
Plasmid#68137Purposestorage vector for BASIC part: KanDepositorInsertKanamycin
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNP561
Plasmid#222723PurposeTarget and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 RecombinaseDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-NP
Plasmid#165090Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSG25-pBAD-tetO-deGFPssrA
Plasmid#102452PurposeExpresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.DepositorInsertdeGFP
UseTagsssrAExpressionBacterialMutationPromoterpBAD-tetOAvailable sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-FL
Plasmid#128415PurposeExpress INTS3 full-length in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
UseTagsGSTExpressionBacterialMutationfull-lengthPromotertac promoterAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB168
Plasmid#101671PurposeOpto-T7RNAP*(563-F1), equal expr.DepositorInsertOpto-T7RNAP*(563-F1), equal expr.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAbaMCi-GFP-BsaI_pJMP2748
Plasmid#208888PurposeAcinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).DepositorInsertdCas9, sgRNA expression cassette, sfGFP
UsePir dependent origin of replicationTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only