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31,059 results

Showing: 5041 - 5080 of 31059 results

  13. pMV306DIG13+FFlucRT

    Plasmid
    #49998
    Purpose
    Integrase removed to increase stability of the plasmid in mycobacteria
    Depositor
    Brian Robertson
    Article
    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2
    Insert
    G13 promoter + Firefly luciferase Red Thermostable
    Use
    Mycobacteria integrating vector
    Expression
    Bacterial
    Mutation
    Codon optimized for Mycobacterium tuberculosis wi…
    Promoter
    G13
    Available Since
    May 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    49998-plasmid-map-sequence-id-74812

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  17. pCS2TAL3-DD

    Plasmid
    #37275
    Purpose
    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)
    Depositor
    David Grunwald
    Article
    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub
    Type
    Empty backbone
    Tags
    Flag, Fok I DD, Tal-C, and Tal-N
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    June 27, 2012
    Availability
    Academic Institutions and Nonprofits only
    37275-plasmid-map-sequence-id-46872

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  18. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept

    Plasmid
    #182424
    Purpose
    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteins
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396
    Inserts
    VNp-LZ-mNeongreen-Etanercept (TNFRSF1B Human)
    VNp-LZ
    Tags
    Vesicle Nucleating peptide (VNp), and Vesicle Nuc…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    182424-plasmid-map-sequence-id-360472

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  19. pTXBI-mClover-YTH domain

    Plasmid
    #177123
    Purpose
    Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Article
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTH domain) (YTHDC1 Human)
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    YTH domain only (IDR1 and IDR2 deletion)
    Promoter
    T7
    Available Since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    177123-plasmid-map-sequence-id-348690

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  21. AviTag-SpyTag003-MBP

    Plasmid
    #184227
    Purpose
    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    AviTag-SpyTag003-MBP
    Tags
    AviTag, His6, SpyTag003, and Thrombin cleavage si…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184227-plasmid-map-sequence-id-361606

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  26. pRSET.GltI253-cpGFP.L1LV/L2NP

    Plasmid
    #41733
    Purpose
    a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    6xHis, T7 epitope, and Xpress tag
    Expression
    Bacterial
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    T7
    Available Since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41733-plasmid-map-sequence-id-56305

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  28. pOPS0381

    Plasmid
    #133231
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133231-plasmid-map-sequence-id-282538

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  29. pET30a-His6-LOXCATmut

    Plasmid
    #140085
    Purpose
    A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutation in H265A and R268A in LOX (used original…
    Promoter
    T7
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140085-plasmid-map-sequence-id-311165

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  30. cr3Dd

    Plasmid
    #184862
    Purpose
    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    Cas9, bet, exo
    Tags
    Cas9 ssrA degradation tag
    Expression
    Bacterial
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    184862-plasmid-map-sequence-id-366226

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  36. pCS2TAL3-RR

    Plasmid
    #37276
    Purpose
    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)
    Depositor
    David Grunwald
    Article
    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub
    Type
    Empty backbone
    Tags
    C terminal Tal From pTAL3, FokI RR, HA, and N-ter…
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    June 27, 2012
    Availability
    Academic Institutions and Nonprofits only
    37276-plasmid-map-sequence-id-46873

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  37. pJ251-GERC

    Plasmid
    #47441
    Purpose
    Expresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.
    Depositor
    George Church
    Insert
    Gene Expression Reporter Construct
    Expression
    Bacterial
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47441-plasmid-map-sequence-id-67511

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  40. pRMC2-sec:msfGFP

    Plasmid
    #194914
    Purpose
    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Expression
    Bacterial
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194914-plasmid-map-sequence-id-384625

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Showing: 5041 - 5080 of 31059 results