32,776 results
-
Plasmid#154074PurposeExpresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coliDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only
-
pJMP1159
Plasmid#119250Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-GreenPy1Highest
Plasmid#239049PurposeBacterial expression of highest affinity green fluorescent pyruvate biosensor GreenPy1HighestDepositorInsertGreenPy1Highest
ExpressionBacterialAvailable SinceJune 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKJ0463
Plasmid#228715PurposeBacterial expression of epT7 RNAP; for protein production and purificationDepositorArticleInsertSumo_epT7
ExpressionBacterialMutationT3M/G47A/E643GAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
Sperm whale Mb 64Y68F in pVP80K
Plasmid#86956PurposeIncreases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).DepositorInsertMb 64Y68F
ExpressionBacterialMutation64Y68FPromoterT5Available SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET_His6-SUMO-TEV-TtCsm6
Plasmid#172486PurposeBacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.DepositorInsertT. thermophilus Csm6 (codon optimized) (csm6 Synthetic)
TagsHis-SUMO-TEVExpressionBacterialPromoterT7 promoter and lac operatorAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)_NADP-Snifit
Plasmid#113913PurposePlasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+DepositorInsertNADP-Snifit
ExpressionBacterialPromoterT7Available SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTOX10
Plasmid#127455PurposeEncodes YhaV toxin. Gent-RDepositorInsertyhaV toxin
ExpressionBacterialPromoterpRhaAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-GST-FBW7 alpha
Plasmid#197456PurposeThe plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.DepositorInsertFBW7 (FBXW7 Human)
UseIn vitro translationTagsGSTExpressionBacterialMutationSee depositor comments belowPromoterT7Available SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC-tag-C
Plasmid#108077PurposeGateway compatible destination vectors containing StrepIII/HA/BioIDDepositorAvailable SinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Grb2 (XSB363)
Plasmid#78505PurposeConstruct for expressing Grb2 in E. ColiDepositorInsertGrb2 (GRB2 Human)
TagsGST followed by a prescission protease claevage s…ExpressionBacterialAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB508-cTP-mCherry
Plasmid#213862PurposeBinary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)DepositorInsertPlastid transit peptide fused mCherry-UnaG
ExpressionBacterial and PlantAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV02
Plasmid#212156PurposePlasmid expressing secondary alcohol dehydrogenase under constitutive promoter PgyrA control.DepositorInsertsecondary alcohol dehydrogenase
ExpressionBacterialPromoterPgyrAAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
Py-MDR2-NBD
Plasmid#25590PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPy-MDR2-NBD
TagsHisExpressionBacterialAvailable SinceAug. 25, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL403
Plasmid#119953PurposeExpresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.DepositorInsertsgpps
limS
ExpressionBacterialMutationN-terminal truncation of 56 amino acid residues (…Available SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
PYCR1
Plasmid#38842PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFE-Selo-FI-prk
Plasmid#162698PurposeExpresses S. elongatus Form IB rubisco and S. elongatus PrkDepositorInsertS. elongatus Form IB rubisco and S. elongatus Prk
TagsN terminal 6x His tag on PRKExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF-5xT7-TtCsm
Plasmid#128572PurposeFor expression of T. thermophilus Csm in E. coli (Csm1-5)DepositorInsertsCsm1
Csm2, Csm3, Csm4, Csm5
TagsHRV 3C protease cleavage site and 10xHis tag on C…ExpressionBacterialMutationCodon optimized for expression in E. coli and Cod…PromoterT7 promoter, lac operatorAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pISCba-reRNA
Plasmid#226823PurposeExpressing ISCba controled by PlacM promoter, guide RNA controled byPJ23119 promoterDepositorInsertISCba and its corresponding guide RNA
ExpressionBacterialAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pISClsp3-reRNA
Plasmid#226824PurposeExpressing ISClsp3 controled by PlacM promoter, guide RNA controled by PJ23119 promoterDepositorInsertISClsp3 and its corresponding guide RNA
ExpressionBacterialAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpG-HsNot9_19-285_AF
Plasmid#148733PurposeBacterial Expression of HsNot9_19-285DepositorInsertHsNot9_19-285 (CNOT9 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-HsNot1iso1_1356-1581_V
Plasmid#147779PurposeBacterial Expression of HsNot1iso1_1356-1581DepositorInsertHsNot1iso1_1356-1581 (CNOT1 Human)
ExpressionBacterialAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-30m
Plasmid#13556DepositorInsertFLIPPi T22A
TagsECFP and EYFPExpressionBacterialMutationT22A in binding proteinAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJMP1189
Plasmid#119257Purposerfp "test" strainDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogCatcher-MBP
Plasmid#171928PurposeExpresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogCatcher-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationDogCatcher added to N-terminus of MBP via a GSGES…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-N1
Plasmid#125547PurposeGateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment1 attached to n-terminus of Gatew…ExpressionBacterial and MammalianPromotersynthetic promoter containing yeast, mammalian, a…Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1CAN
Plasmid#178032PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialPromoterT7LacIAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
KEAP1 (KEAP1A-c029)
Plasmid#110247PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJUMP25-1A(sfGFP)
Plasmid#126972PurposeLevel 1 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only