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Bacterial Expression

29,747 results

Showing: 6701 - 6750 of 29747 results

  5. pBT3007

    Plasmid
    #122538
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a early endosome localized gene (rab-5) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::rab-5 (rab-5 Synthetic, Nematode)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122538-plasmid-map-sequence-id-238129

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  8. pHS1950

    Plasmid
    #205983
    Purpose
    Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Inserts
    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    CRISPR array
    Use
    Tags
    14xHis and TwinStrep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (proteins), pJ23119 (crRNA)
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205983-plasmid-map-sequence-id-415374

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  21. pGMCK-T38S38-750-luxAB-DAS+4

    Plasmid
    #49518
    Purpose
    Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    T38S8-P750-luxAB-DAS+4
    Use
    Tags
    DAS+4 (AANDENYSENYADAS)
    Expression
    Bacterial
    Mutation
    Promoter
    P750
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49518-plasmid-map-sequence-id-73896

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  22. pXWJ109As(RS-te-AAV)

    Plasmid
    #123156
    Purpose
    For TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, ParsR, PhrpLE
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123156-plasmid-map-sequence-id-240600

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  25. pDB038a

    Plasmid
    #99211
    Purpose
    Accessory plasmid for PACE of PylRS variants
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 29 and 184 of gIII
    Promoter
    Ppsp and PproK
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99211-plasmid-map-sequence-id-192359

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  29. KLC2

    Plasmid
    #40735
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KLC2 (KLC2 Human)
    Use
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pRN3P-NLS-EGFP-Btgh-3NLS

    Plasmid
    #194469
    Purpose
    Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Article
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    194469-plasmid-map-sequence-id-384873

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  32. (SUMO)5-(SIM)10

    Plasmid
    #126923
    Purpose
    Bacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    internal BamHI site between (SUMO)5 and (SIM)10
    Promoter
    tac
    Available since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126923-plasmid-map-sequence-id-255302

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  33. pPBC-G3-tdT

    Plasmid
    #62814
    Purpose
    pPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked GCaMP3 and tdTomato under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-GCaMP3-IRES-tdTomato-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    62814-plasmid-map-sequence-id-121226

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  35. katushka-FMDV-P2A-pTRIP

    Plasmid
    #163522
    Purpose
    Used to construct lentivirus to express katushka in mammalian cells with option for a second insert
    Depositor
    Thomas Weber
    Insert
    stopless Katushka-P2A
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163522-plasmid-map-sequence-id-321575

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  36. pGBW-m4046979

    Plasmid
    #145611
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp7
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145611-plasmid-map-sequence-id-292502

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  40. peSCBE3-NG-HF1-Hypa

    Plasmid
    #218166
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218166-plasmid-map-sequence-id-433270

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  41. pBDK

    Plasmid
    #135190
    Purpose
    Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    KanR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    cat
    Available since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135190-plasmid-map-sequence-id-273774

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  47. HS_NAM-B1

    Plasmid
    #154064
    Purpose
    Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    Hygromycin phosphotransferase
    Cre recombinase
    LoxP-flanked GUS and TtNAM-B1
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Tags
    Expression
    Bacterial and Plant
    Mutation
    The TtNAM-B1 gene sequence was domesticated to re…
    Promoter
    HvHSP17, OsAct, and ZmUbi
    Available since
    Sept. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154064-plasmid-map-sequence-id-313309

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  48. pGNW6

    Plasmid
    #122093
    Purpose
    suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells
    Depositor
    Pablo Ivan Nikel
    Article
    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122093-plasmid-map-sequence-id-243567

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Showing: 6701 - 6750 of 29747 results