31,059 results
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Plasmid#171702PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-PTS
Plasmid#108953Purposeheterologous expression of PTS of P. cablin in E. coliDepositorInsertPatchoulol synthase (PTS)
TagsHis6-TagExpressionBacterialAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETSG
Plasmid#159418PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coliDepositorInsertthermostable GFP (TGP)
Tagspolyhistidine (poly-His)ExpressionBacterialAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-wt
Plasmid#53095PurposeExpresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.DepositorInsertLOV(wt)-6aa-DAD
Tags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in VenusAvailable SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-PAmCherry-KanMX6
Plasmid#87026PurposeTag S. pombe genes in C terminal with PAmCherryDepositorInsertPAmCherry
ExpressionBacterial and YeastAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-134
Plasmid#118520PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmOrange2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-MAP
Plasmid#66999PurposeExpresses malE-MAP in E. coliDepositorInsertmalE-MAP
TagsHis6 and malE-Factor XaExpressionBacterialPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBacPuroR-NeoR
Plasmid#34921DepositorInsertsBacterial promoter::PuroR::bacterial 3p flank
Bacterial promoter::NeoR::bacterial 3p flank
ExpressionBacterialAvailable SinceMay 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAH121
Plasmid#164901PurposeThe pAH121 plasmid [Haldimann and Wanner 2001] is the phi21 helper plasmid necessary for genomic integration of the pCYMR expression plasmid.DepositorInsertphi21 Integrase
ExpressionBacterialAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP3416
Plasmid#128720PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopT1-1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5534
Plasmid#128723PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopX1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Rnl2(1-249)K227Q
Plasmid#14072DepositorInsertRnl2(1-249)
TagsHis (10x)ExpressionBacterialMutationchanged Lys227 to Glutamine Gly112 is actually C…Available SinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKERep
Plasmid#211826PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.DepositorInserteGFP
ExpressionBacterialPromoterT5Available SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKESa
Plasmid#211828PurposeLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.DepositorInserteGFP
ExpressionBacterialPromoterT5Available SinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
MZB174
Plasmid#228487Purpose10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expressionDepositorTypeEmpty backboneTags10xHis-SUMO(Smt3)-GST-HRVExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGP-Tn7-Pcym
Plasmid#225280PurposeMini-Tn7 pGP-Tn7-Cm vector with inducible Pcym cumate promoter for the inducible/controlled expression of target genes inserted into the attTn7 chromosomal site.DepositorTypeEmpty backboneExpressionBacterialPromoterPcym cumate (p-isopropylbenzoate)-inducible promo…Available SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPPC019.AAV
Plasmid#171147PurposeExpression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmidDepositorInsertshAAVS1 scRNA
J1-BBa_J23117-mRFP
UseCRISPRExpressionBacterialPromoterBBa_J23119 and J1-BBa_J23117Available SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEK36
Plasmid#210598PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
TagsmClover3ExpressionBacterial and YeastPromoterSpH2BproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-WT-N’His
Plasmid#117701PurposeExpression of WT mouse SOD1 with N- terminal His tagDepositorAvailable SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-BRT17
Plasmid#45358DepositorInsert17 Repeat Beta Roll Tag
TagsMaltose Binding Protein (MBP)ExpressionBacterialPromoterPtacAvailable SinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
Dual opposing T7 promoters reporter plasmid
Plasmid#156457PurposeA mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.DepositorInsertNeoR/KanR
ExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7Available SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIV298
Plasmid#204957PurposeAMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection markerDepositorInsertsMad7
pyrG
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and native pro…Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T HHARI RBR
Plasmid#31254DepositorInsertHuman Homologue of Ariadne (HHARI) (ARIH1 Human)
TagsGSTExpressionBacterialMutationRBR domain residues 177-395Available SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-Leum
Plasmid#44316DepositorInsertUAS1B8-Leum promoter
UseSynthetic BiologyExpressionBacterialMutationN/APromoterUAS1B8-LeumAvailable SinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
1457_pDEST_miniTol2_R4-R2_Cryst_mCherry
Plasmid#171796PurposepDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPPC009.106
Plasmid#171142PurposeExpression of J106 scRNA on pBBR1-KmR plasmidDepositorInsertJ106 scRNA
UseCRISPRExpressionBacterialPromoterBBa_J23119Available SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
R701-X54-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D
Plasmid#159562PurposeE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12DDepositorInsertHis6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D (KRAS Human)
TagsHis6-MBP-tev-AviExpressionBacterialMutationG12DAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET His6 StrepII TEV LIC cloning vector (2HR-T)
Plasmid#29718DepositorTypeEmpty backboneTags6xHis, Strep Ii, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESRRA_Hormone-recep
Plasmid#109908PurposeProtein expression and purification of ESRRA_Hormone-recepDepositorInsertESRRA_Hormone-recep (ESRRA Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSF-FGE
Plasmid#102615PurposeInducible expression of Formylglycine-generating enzymeDepositorInsertFormylglycine-generating enzyme
ExpressionBacterialPromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJDC181
Plasmid#60853PurposeExpresses click beetle red luciferaseDepositorInsertClick beetle red luciferase (optimized for Mycobacteria)
ExpressionBacterialMutationcodon optimized for MycobacteriaPromoterhsp60Available SinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUdO4z
Plasmid#210253Purposelow-copy plasmid for bacterial expressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUdO2z
Plasmid#210251Purposemedium-copy plasmid for bacterial expressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUdO1c
Plasmid#210230Purposehigh-copy plasmid for bacterial expressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSJ111
Plasmid#78707PurposeModular vector for constitutive secreted amylase reporter expressionDepositorInsertamyEopt
ExpressionBacterialPromoterPvegAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSHDY-PL03-mVenus_tetR
Plasmid#137663PurposeConjugative vector derived from pSHDY. Constitutive expression of the repressor tetR, as well as aTc-inducible expression of mVenus.DepositorInsertPL03:mVenus
ExpressionBacterialPromoterPL03Available SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbB6c-GFP
Plasmid#35356DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialPromoterLlacO-1Available SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNS207
Plasmid#35041DepositorInsertcytochrome b562
ExpressionBacterialPromoterlacAvailable SinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
ACAT1
Plasmid#25510PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 30, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
NpuDnaEc-cGCaMP2
Plasmid#34851DepositorInsertNpuDnaEc-cGCaMP2
ExpressionBacterial and MammalianPromoterCMV, T7 lacAvailable SinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFTC1
Plasmid#64951Purposesource of TcR FRT cassetteDepositorInsertFRT-TcR-FRT
ExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
HisMS2_VLP_RSV
Plasmid#175958PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]DepositorInsertsP gene
M gene
ExpressionBacterialPromoterT7Available SinceSept. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDF_LuxR
Plasmid#140039PurposeConstutitive expression of LuxR protein.DepositorInsertpLuxL and LuxR
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-PBD
Plasmid#60880PurposeBacterially expressed GST-tagged human polo-like kinase 1 PBD AA365-653DepositorAvailable SinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM22C
Plasmid#135488PurposeAgrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hphDepositorInserthph SpunH2A/Bpr LifeAct-tdTomato
TagsLifeActExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_HSPA5-Halo
Plasmid#175326PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-jAspSnFR3
Plasmid#203458PurposejAspSnFR3 in T7 bacterial expression vectorDepositorInsertjAspSnFR3
TagsHis-tagExpressionBacterialPromoterT7Available SinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SPF45
Plasmid#110044PurposeProtein expression and purification of SPF45DepositorInsertSPF45 (RBM17 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRBV18
Plasmid#123452Purposeconstruction of TCRDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRBV15
Plasmid#123450Purposeconstruction of TCRDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only