29,754 results
-
Plasmid#128705PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopA1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-C9ORF80
Plasmid#128418PurposeExpress C9ORF80 in E. coli with a His tag (C-terminal)DepositorInsertC9ORF80 (INIP Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
YopH-pNIC-Bsa4
Plasmid#92063PurposepNIC expression venctor encoding gene for YopHDepositorInsertYopH
UseTags6x His TagExpressionBacterialMutationCodon optimized E. coliPromoterAvailable sinceJan. 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
Aurein1.2_(+36GFP)-Cre
Plasmid#71748PurposeAurein1.2+36GFP-Cre fusion expression in E. ColiDepositorInsert(GGS)9_Aurein1.2+36GFP-Cre
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
SUMO_TwinStrep_Csx30
Plasmid#197611PurposeFor protein purification of Csx30DepositorInsertSUMO_TwinStrep_Csx30
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBTL-4
Plasmid#22808DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-26
Plasmid#172751PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-26
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_RFP
Plasmid#136979PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker RFP
UseTagsExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…PromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
4Z3C (TET3)
Plasmid#119969PurposeBacterial expression for structure determinationDepositorInsertTET3 (TET3 Human)
UseTags6xHis-TEVExpressionBacterialMutationcontains amino acids 48-98PromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSW002-Pc
Plasmid#108234PurposeBroad host bacterial expression vector with constitutive Pc promoter.DepositorInsertPc promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28GG
Plasmid#104655PurposeLevel 2 cloning vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS ColI alpha2
Plasmid#19207DepositorInsertAlpha 2 type I collagen (COL1A2 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat5A-SH2
Plasmid#46515DepositorInsertStat5A (STAT5A Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 582-693PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSC5GGv2
Plasmid#188604PurposepSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET GFP domain 11 LIC cloning vector (GFP C-11)
Plasmid#30183DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), GFP domain 11, and TEV cl…ExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_2
Plasmid#118226PurposeEmpty E. coli - B. burgdorferi shuttle vector; kanamycin resistantDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVRb20_992
Plasmid#49714DepositorInsertsfgfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPecf20_992Available sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1
Plasmid#32466DepositorInsertG-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFQAQLGGL…ExpressionBacterialMutationGCaMP3 K119I/L173Q/S404G/E430VPromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
-
pJB166
Plasmid#86998PurposeCRISPR/Cas9 in fission yeast using fluoride selectionDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD33MevT
Plasmid#17814DepositorInsertMevT
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pQE80_T5_Z17-ehaA_LacI
Plasmid#186313PurposePlasmid for inducible E coli expression of the Z17 helixCAM.DepositorInsertZ17-ehaA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCPP3417
Plasmid#128724PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopY1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
KIF3A560GFP
Plasmid#129769PurposeExpresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP taggedDepositorInsertKIF3A (Kif3a Mouse)
UseTagsGFP and His6ExpressionBacterialMutationTruncation at 359, fused to kinesin-1 starting Al…PromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(D3A: D175A D28A D9A)
Plasmid#90090PurposeExpresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationD175A D28A D9APromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CH10-GG
Plasmid#215807PurposeCloning of target gene with C terminal non cleavable His10 tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET26-MBP-GLIC
Plasmid#20887DepositorInsertpentameric ligand gated ion channel from Gloeobacter violaceus PCC 7421
UseTagsHis, MBP, and pelBExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAD39_mIRG1_4-462_pvp008
Plasmid#124873PurposeExpresses mouse CAD (Irg1) in E.coliDepositorInsertcis-aconitate decarboxylase (Acod1 Mouse)
UseTagsStrepTag and TEV siteExpressionBacterialMutationdeleted amino acids 1-3 and 463-end.PromoterT7Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-HSPB8
Plasmid#34710DepositorInsertheat shock 22 kDa protein 8 (HSPB8 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
-
pBbB9a-GFP
Plasmid#126112PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23113Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-HIS-CBM-Xmod-DocIII
Plasmid#91698PurposeCBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGGDepositorInsertGGG-HIS-CBM-Xmod-DocIII
UseTagsHIS-Tag and Sortase-TagExpressionBacterialMutationCysteine deletion in CBM C63SPromoterT7Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJB007
Plasmid#98995PurposeExpress FtsI tagged with tagRFP-t in E coliDepositorInsertFtsI
UseTagsTagRFP-TExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL4529 (pTnpA)
Plasmid#210869PurposeExpresses GstTnpA from pLac promoterDepositorInsertGstTnpA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL5200 (pDonor_ISGst3)
Plasmid#210875PurposeHarbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458DepositorInsertISGst3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
GST-TPX2(1-43)
Plasmid#69107PurposeFor bacterial expression of human TPX2(1-43aa) tagged with GST.DepositorInsertTargeting Protein for Xklp2 (TPX2 Human)
UseTagsGSTExpressionBacterialMutationAmino acids 1-43 expressed.PromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM-41-HsPABPC1_J
Plasmid#146644PurposeBacterial Expression of HsPABPc1DepositorInsertHsPABPc1 (PABPC1 Human)
UseTagsExpressionBacterialMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
pET_SZ1-RGG-RGG
Plasmid#124942PurposeExpression of SZ1-RGG-RGGDepositorInsertSYNZIP1-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-153
Plasmid#118535PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPEPY-Plac
Plasmid#122634PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAP2
Plasmid#171208Purposebacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV proteaseDepositorInsertpgl-3 (pgl-3 Nematode)
UseTagsMBP::HIS::TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACT2-CAS9c
Plasmid#51055PurposeExpress Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.DepositorInsertCas9c
UseCRISPRTagsSV40 NLSExpressionBacterial and YeastMutationPromoteryeast ADH1 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG_N2N3-HIS-ybbr
Plasmid#101238PurposeSdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertSdrG_N2N3
UseTagsH3V 3C, HIS, and ybbrExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST-Ki
Plasmid#44563Purposefor integrative constitutive expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMD1 anti-GFP nanobody-Tn5
Plasmid#215567PurposeExpresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tagDepositorInsertanti-GFP nanobody-Tn5 fusion
UseTags3xFlag and intein-CBDExpressionBacterialMutationPromoterT7Available sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMMK803
Plasmid#207138PurposeAssembled Tn10 with CP25 promoter driving gfpDepositorInsertTn10-CP25-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterCP25Available sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -