31,059 results
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pSMT3-FBF2
Plasmid#166972PurposeExpresses FBF-2 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
TagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Aβ(M1-42/C18C33)
Plasmid#173759PurposeExpresses the AβC18C33 peptide.DepositorInsertAβ(M1-42/C18C33) (APP Human)
ExpressionBacterialMutationchanged valine 18 to cysteine; changed glycine 33…PromoterT7Available SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6T7
Plasmid#71462PurposeExpresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)DepositorTypeEmpty backboneUseCRISPRExpressionBacterial and MammalianPromoterhuman U6/phage T7Available SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (ISF1)
Plasmid#61929PurposeExpression of hUCH37 (ISF1) in E.coliDepositorAvailable SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNS38-SadCas9-SNAP
Plasmid#113718PurposeBacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationD10A, N580AAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2 PCNA GFP
Plasmid#105937Purposein vitro transcription, cell cycle nuclear markerDepositorAvailable SinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CLC-EYFP
Plasmid#38011DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
TagsEYFP and SNAP26mExpressionBacterial and MammalianPromoterCMVAvailable SinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
htpG1-GFP
Plasmid#109387PurposeGFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG1-GFP
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-SynTetOff-GFP
Plasmid#190230PurposeGateway entry vector, high-level expression of EGFP in neuronal cellsDepositorInsertEGFP
ExpressionBacterialAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pASR103
Plasmid#122637PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
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poRFP-oGFP
Plasmid#154130PurposeExpresses orthogonal gfp and orthogonal rfp genes in bacterial cellsDepositorInsertsorthogonal gfp
orthogonal rfp
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKJ1712
Plasmid#21870DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO PCGF2
Plasmid#69791PurposeGateway entry vector for PCGF2DepositorInsertPCGF2 (PCGF2 Human)
ExpressionBacterialAvailable SinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEND-400_Cacnes_CAMP2
Plasmid#225606PurposeSuicide C. acnes vector derivative of pEND-350 optimized for recombination into the CAMP2 locus, with BxbI recombination sites flanking EryR resistance cassetteDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pED14xS-4
Plasmid#134802PurposeIPTG inducible expression of S-TAGA-Evo3 qtRNADepositorInsertS-TAGA-Evo3 qtRNA
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbE7k-RFP
Plasmid#35315DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-2_LC3A
Plasmid#73946PurposeFor recombinant expression of human LC3A/MAP1LC3A in E. coliDepositorAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-CBX1-eReader
Plasmid#135089PurposeBacterial expression of engineered CBX1 with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered
Tags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
ELK1_pet28a
Plasmid#131647PurposeBacterial expression of ETS gene ELK1DepositorAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSIBR003
Plasmid#177666PurposeSIBR Intron 3 (Int3) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterlacUV5Available SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pet28a-His6-Halo-Tev-Keap1
Plasmid#62456PurposeHis6-halo tagged human Keap1 protein for E.coli expressionDepositorAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAD-SPS6-Pman
Plasmid#133906PurposeFluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.DepositorInsertsfGFP(SPS6)
UseE.coli/bacillus spp. shuttle vectorExpressionBacterialPromoterPmanAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRC-LIC
Plasmid#62343PurposeUsed for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.DepositorTypeEmpty backboneExpressionBacterialPromotertac promoter from pKK233-2Available SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET-A3A(N57Q)-BE3
Plasmid#158157PurposeT7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLSDepositorInsert6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
Tags6xHis tag on N-terminusExpressionBacterialPromoterT7Available SinceNov. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYTK080
Plasmid#65187PurposeEncodes ZeocinR as a Type 6 part to be used in the Dueber YTK systemDepositorInsertZeocinR
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWVR5
Plasmid#167509PurposeBroad-Host range expression vector, pSa origin, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertVanRAM-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPVanCCAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCRISPRed pRSETB
Plasmid#159454PurposeExpresses the pH-resistant long Stokes shift red fluorescent protein mCRISPRedDepositorInsertmCRISPRed
Tags10 x His-TagExpressionBacterialPromoterT7Available SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
p4028 GST-E6AP
Plasmid#8655DepositorAvailable SinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMRE161
Plasmid#118509Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
ExpressionBacterialAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris sp2 CRISPR/pACYCDuet-1
Plasmid#81186PurposeExpresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coliDepositorInsertSynthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
UseCRISPRTagsNoneExpressionBacterialPromoterT7Available SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔPS-mCherry-PCS/PM,CS-GFP
Plasmid#134357PurposeReporter plasmidDepositorInsertsGFP
mCherry
ExpressionBacterialAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
K980
Plasmid#129762PurposeExpresses Drosophila Kinesin-1 full-length for microtubule gliding assaysDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28aAviHisCD72cCTLD
Plasmid#129066Purposegeneration of biotinylated mouse CD72c CTLD protein using bacteriaDepositorInsertCD72c (Cd72 Mouse)
ExpressionBacterialAvailable SinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-hTRPT1-iso1
Plasmid#184239PurposeFor purification of N-terminally His-tagged TRPT1 from bacteriaDepositorAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRC072
Plasmid#225982PurposeJ23110_buj_sfGFP_buj_mRFPDepositorInsertsfGFP (with buj RBS), mRFP (with buj RBS)
ExpressionBacterialPromoterJ23110Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PCMT1_PCMT
Plasmid#109932PurposeProtein expression and purification of PCMT1_PCMTDepositorInsertPCMT1_PCMT
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human full-length LIC1
Plasmid#74597Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tagDepositorInsertfull length human dynein light intermediate chain 1 (DYNC1LI1 Human)
TagsN-terminal GST and Strep IIExpressionBacterialPromoterT7Available SinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)
Plasmid#181791PurposeExpresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28GST-LIC
Plasmid#26101PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTags6XHis, GST, and Thrombin CLeavage SiteExpressionBacterialAvailable SinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSET-B YFP
Plasmid#108856PurposeEncodes for the fluorescent protein enhanced YFP (eYFP)DepositorInserteYFP
ExpressionBacterialAvailable SinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
PGR-Blue
Plasmid#68374PurposeTerminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.DepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assemblyExpressionBacterialPromoterpBADAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-MinD_MinE
Plasmid#133621PurposeExpression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.DepositorAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPath-BGC08-SGE
Plasmid#200006PurposeBGC08 pathway. SGEDepositorInsertPathways
ExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
ZP46 Prickle-2
Plasmid#16896DepositorInsertPrickle-2 (prickle2b Zebrafish)
ExpressionBacterialAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-HygroR-T2A-TetOn3G
Plasmid#179887PurposeExpresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)DepositorInsertTetOn3G
UseLentiviralTagsHygromycin resistanceExpressionBacterial and MammalianPromoterEF1aAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
G3BP1A
Plasmid#42480PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertG3BP1A
TagsHis6-TEVExpressionBacterialMutationSee CommentsAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDSG594
Plasmid#214221Purposecompetition strain 10 integrase plasmidDepositorInsertPhlF, A118
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only
