32,669 results
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Plasmid#25510PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 30, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits
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UHRF1
Plasmid#28163PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUHRF1 (UHRF1 Human)
TagsHis tag with TEV cleavageExpressionBacterialMutationtandem tudor domainAvailable SinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-mOct4 Full length(FL)
Plasmid#206394PurposeExpresses Oct4 with a 6xHis tag for protein purificationDepositorAvailable SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIK
Plasmid#126554PurposeBroad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selectionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP6534
Plasmid#128735PurposeGateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal HA tagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET11b-HA2
Plasmid#165992PurposeInfluenza HA ministem with removable HisTagDepositorInsertHA2
TagsFoldon and HisTagExpressionBacterialPromoterT7Available SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM18
Plasmid#135484PurposeAgrobacterium T-DNA with SpunH2Bpr driving hph-tdTomatoDepositorInsertSpunH2Bpr hph-tdTomato ADH1t
TagstdTomatoExpressionBacterial and YeastPromoterSpunH2BprAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B16-TEF(504)
Plasmid#44371DepositorInsertUAS1B16-TEF(504) promoter
UseSynthetic BiologyExpressionBacterialMutationN/APromoterUAS1B16-TEF(504)Available SinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pProEx Rab5C(S30C) CL
Plasmid#224273PurposeProtein purification of Rab5C (S30C)DepositorAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHT-trxA.ads
Plasmid#47383PurposeExpresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteriaDepositorInsertads
Tags6xHis and trxAExpressionBacterialPromoterPgracAvailable SinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-X(5010)B1
Plasmid#168019PurposeExpresses X(5010)B1-6xHis in bacterial cellsDepositorInsertX(5010)B1-6xHis
TagsHisx6ExpressionBacterialAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_AT
Plasmid#196243PurposeBackbone plasmid for cloning sgRNADepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterJ23119Available SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pnCas9-AID
Plasmid#196242PurposePlasmid expressing the nCas9-AID fusionDepositorInsertnickase Cas9 fused to AID
UseCRISPRExpressionBacterialPromotertac promoterAvailable SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-3'TwStrep
Plasmid#208053PurposeEmpty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.DepositorTypeEmpty backboneTagsTwin StrepExpressionBacterialPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDD132
Plasmid#119885PurposeIntegration vector into the ROCI-3 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance markerDepositorInsertBbaJ23104_EYFP
ExpressionBacterialAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDCAF3-IS5
Plasmid#65220PurposeExpresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.DepositorInsertsndmA
ndmB
ndmC
ndmD
gst9
chlR
UseSynthetic BiologyExpressionBacterialMutationChanged start codon from gtg to atg and Changed s…PromoterBBa_J23100Available SinceJan. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
TH
Plasmid#39079PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-A3A(N57Q)-BE3
Plasmid#158157PurposeT7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLSDepositorInsert6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
Tags6xHis tag on N-terminusExpressionBacterialPromoterT7Available SinceNov. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQLink-GST-4xCTD
Plasmid#138471PurposeExpresses GST-tagged 4xCTD heptapeptide repeat in bacteriaDepositorAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
(SIM)5
Plasmid#126947PurposeBacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds.DepositorInsert5 repeats of SUMO interacting motif (SIM) from PIASx, each separated by (GGS)4 (PIAS2 Human)
ExpressionBacterialPromoterunknownAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-TEX264_wt-His6
Plasmid#220214PurposeBacterial expression of wild type TEX264, C-terminal His6 tagDepositorAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3523 pGEM HPV-11 E6
Plasmid#10854DepositorInsertHPV11 E6
TagsHAExpressionBacterialAvailable SinceOct. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAP84
Plasmid#86554PurposeExpresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficileDepositorInsertPveg-sLucopt
UseLuciferase and Synthetic BiologyExpressionBacterialPromoterPvegAvailable SinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-gp78c/2767
Plasmid#11429DepositorAvailable SinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221rAPOBEC1-T7RNAP
Plasmid#167979PurposeExpresses fusion protein rAPOBEC1-T7RNAP in E. coliDepositorInsertrAPOBEC1-T7RNAP (T7p07 Synthetic)
TagsT7 tag and T7RNAPExpressionBacterialPromoterTetR-PtetAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLZ12Km2-P23R:TA:ffluc
Plasmid#88900PurposeToxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expressionDepositorInserttoxin-antitoxin + firefly luciferase
UseLuciferaseExpressionBacterialAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAT224
Plasmid#134892PurposeBacterial expressionDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_1UXZ
Plasmid#234166PurposeHeterologous protein expression of cellulase B in Escherichia coliDepositorInsert1UXZ
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pdCas9-omega
Plasmid#114632PurposeAddgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAPDepositorInsertdCas9-omega
UseCRISPRExpressionBacterialPromoterTet promoterAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
ECHS1
Plasmid#38845PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pB1H2w2-Prd
Plasmid#18038DepositorInsertPaired (prd Fly)
ExpressionBacterialMutationPaired is simply used as an insert between Kpn1 a…Available SinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDYU1B/mRuby3–α-tubulin
Plasmid#154302PurposeExpresses mRuby3–α-tubulin from actin15 promoter in Dictyostelium discoideumDepositorInsertalpha-tubuin
UseDictyostelium expressionTagsmRuby3ExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
SETD7
Plasmid#40746PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceNov. 14, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFloat.T7_v1
Plasmid#133070PurposeEntry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.DepositorTypeEmpty backboneUseGateway CloningTags3C-His6 and His6-3CExpressionBacterialPromoterT7Available SinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBD-0001
Plasmid#12373DepositorAvailable SinceAug. 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
BMPR2
Plasmid#38972PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-SKM
Plasmid#136551PurposeDox-inducible polycistronic reprogramming lentiviral vector expressing mouse Sox2, Klf4, cMycDepositorUseLentiviralExpressionBacterialPromotertetO-miniCMV (dox-inducible)Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-VCAM1
Plasmid#202471PurposeExpresses the nanobody VCAM1 after phosphate depletionDepositorInsertnanobody VCAM1
Tags6X-HisExpressionBacterialPromoteryibDpAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-trc- YFP
Plasmid#186569Purposep15A ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
ExpressionBacterialPromotertrc promoterAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
EcNusGFL
Plasmid#113122PurposeExpresses full-length E. coli NusGDepositorAvailable SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
MAPK3
Plasmid#73235PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAPK3 (MAPK3 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00069:Pkinase 42-330PromoterT7Available SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPJ23116-MS2-rep
Plasmid#184842PurposeExpression of the rep protein of phage MS2DepositorInsertReplicase gene MS2 phage
UseSynthetic BiologyExpressionBacterialPromoterJ23116Available SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJ191
Plasmid#162668PurposepET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
TagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV125
Plasmid#63191PurposepRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
ACOT4A
Plasmid#42449PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEB2_mScarletI_mVenusNB-MrkH
Plasmid#182291Purposeexpresses c-di-GMP biosensor in E. coliDepositorInsertmVenusNB-MrkH
TagsmVenusNB internal fusion to MrkHExpressionBacterialPromoterproCAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
RhoA
Plasmid#55699Purposeexpression of mouse RhoADepositorAvailable SinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-DARPinOFF7
Plasmid#182398PurposeBacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusionDepositorInsertVNp6-DARPinOFF7
TagsVesicle Nucleating peptide 6 (VNp6)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAT219
Plasmid#134887PurposeBacterial expressionDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-D661A
Plasmid#92236PurposeExpress D661A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-D661A mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationAspartate 661 to AlaninePromoterT7 promoterAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only