29,765 results
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Plasmid#66714PurposeExpresses UBE2S (1-196) in E. coliDepositorInserte2-epf (UBE2S )
UseTagsGST-3CExpressionBacterialMutationPromoterT7Available sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_09
Plasmid#194670PurposeProtein production of the split HaloTag based calcium recorder Caprola_09 in bacteriaDepositorInsertCaprola_09
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
6CDG (GID4)
Plasmid#119961PurposeBacterial expression for structure determinationDepositorInsertGID4 (C17orf39 Human)
UseTags6xHis-TEVExpressionBacterialMutationcontains amino acids 124-289PromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-Tight-Puro_Olig1
Plasmid#64841PurposeA lentiviral vector for the expression of mouse Olig1 under doxycyline controlDepositorInsertOlig1 (Olig1 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PSMD10
Plasmid#31332DepositorInsertPSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTA-attB
Plasmid#18937DepositorInsertphiC31 attB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2008AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-OspC
Plasmid#159998PurposeExpresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cellsDepositorInsertSpyTag003-OspC (20-209) C129S
UseTagsHis6-tag and Thrombin cleavage siteExpressionBacterialMutationC129S mutation improves expression level.PromoterT7Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-attL1-Human ABeta-attR5
Plasmid#160436PurposeEntry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombinationDepositorInsertHomo sapiens amyloid beta precursor protein (APP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Ub11TCA
Plasmid#174517PurposeRecoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitinDepositorInsertUbiquitin
UseTags6xHisExpressionBacterialMutationRecoded with TCA replacing codon 11 of wt ubiquit…PromoteraraBADAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCoofy48
Plasmid#55186PurposeBacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTags6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…ExpressionBacterialMutationPromoterT7Available sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPEP1
Plasmid#61046PurposeBglFusion compatible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME GFP-PCNA
Plasmid#105977Purposeentry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNADepositorInsertPCNA (PCNA Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJUMP27-1A(sfGFP)
Plasmid#126974PurposeLevel 1 vector. sfGFP reporter. Origin pSC101 (low copy number)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_5
Plasmid#60577Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_5
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-ermGFP
Plasmid#27172DepositorInserterythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pATZ-BFP-HA-GFP11
Plasmid#177721PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
UseTagsGFP11, HA, and TagBFPExpressionBacterial and MammalianMutationE342K (ATZ mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-SpyTag003
Plasmid#216282PurposeExpresses SUMO with a C-terminal SpyTag003 in bacterial cellsDepositorInsertSUMO-SpyTag003
UseTagsHis6 and SpyTag003ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-THRX-TEV-GG
Plasmid#215796PurposeCloning of target gene with N terminal His6 Thioredoxin tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAB744
Plasmid#176579PurposeExpressing the whole n-butanol pathway.DepositorInsertphaJ, ter, adhE2, phaA, phaB
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pIKM1
Plasmid#51054PurposeKanamycin and erethromycin resistanceDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 19, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEA183
Plasmid#187873PurposeExpresses eGFP under the virB promoter.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterAgrobacterium virB promoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (pGEX2T(TEV))
Plasmid#80630PurposeBacterial Expression Vector; TEV cleavable GST-tag Internal ID: WISP06-197DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 1-222PromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET PPL His6 MBP LIC cloning vector (2K-T)
Plasmid#37183DepositorTypeEmpty backboneUseTagsHis6, MBP, and PPLExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFAR5
Plasmid#149478PurposeBroad-Host range expression vector, RSF1010 origin, AraC activator/repressor, PBAD promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertAraC-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKJ1267 (aka pAC-alphaGal4)
Plasmid#21869DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
His6tev CBP HAT (1324-1700)
Plasmid#99340Purposebacterial expression of His tagged mouse CBP HAT domain (1324-1700)DepositorInsertmouse CBP HAT domain (1324-1700) (Crebbp Mouse)
UseTagsHis6tevExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ-mNeongreen
Plasmid#182392PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper-mNeongreen fusionDepositorInsertVNp-LZ-mNeongreen
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-36γ
Plasmid#214345PurposeProtein overexpression in bacteriaDepositorInsertIL36G (IL36G Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7·X-nia
Plasmid#122592PurposePlasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion siteDepositorInsertxylS/Pm->Nia
UseSynthetic Biology; Tn7 genomic integrationTagsExpressionBacterialMutationPromoterPmAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Cx43-BioID2-BioID2-HA
Plasmid#176743PurposeExpresses Connexin-43 fused with BioID2 at the C-terminus in astrocytesDepositorInsertCx43-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-distrepII
Plasmid#196667PurposeExpression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsTEV-cleavable di-strep tag IIExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-1_pET29b
Plasmid#215433PurposeExpression construct for UMG-SP-1 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-2_pET29b
Plasmid#215434PurposeExpression construct for UMG-SP-2 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-2
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7C-DNAJB6
Plasmid#177657PurposeRecombinant protein synthesis of human DNAJB6DepositorInsertDNAJB6 (DNAJB6 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQStrep2-ARPC3
Plasmid#31580DepositorInsertActin related protein 2/3 complex, subunit 3 (ARPC3 Human)
UseTagsHis and StrepII-tagExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLC-241
Plasmid#73194PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (mqsR) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertPrhaB-mqsR (mqsR )
UseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOVSD
Plasmid#81033PurposeMammalian expression of the super-dark state of LOV2DepositorInsertLOVSD
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationC450A, L514K, G528A, L531E, N538EPromoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYTK036
Plasmid#65143PurposeEncodes Cas9 as a Type 3 part to be used in the Dueber YTK systemDepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJKW0770
Plasmid#119023PurposeE.coli expression plasmid for PmSPS1DepositorInsertPmSPS1
UseTags8xHisExpressionBacterialMutationPromoterT7Available sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG024
Plasmid#113991PurposeLevel 1 golden gate cloning vector for medium copy number free-living expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET23-PBD(65-150)-N-Cys
Plasmid#13722DepositorInsertPBD(65-150)-N-Cys (PAK1 Human)
UseTags6xHisExpressionBacterialMutationMet-Cys-Gly-PBD(65-150)-PromoterAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pJL1-YPet
Plasmid#102633PurposeIn vitro expression of YPet from the T7 promoterDepositorInsertYPet
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHS0663 pET45b(+) KraIscB-1 TwinStrep
Plasmid#176536PurposeBacterial expression of KraIscB-1-TwinStrepDepositorInsertKraIscB-1
UseTagsTwinStrepExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-521
Plasmid#159688PurposeGateway destination vector for E. coli protein expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFAB808
Plasmid#47853PurposeBIOFAB reporter plasmid for measuring rrnB T1 termination efficiencyDepositorInsertrrnB T1 terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO8Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET His6 TEV cloning vector with BioBrick polycistronic restriction sites (9B)
Plasmid#48284DepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-AGO4
Plasmid#24319DepositorInsertAGO4 (AGO4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only