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31,059 results

Showing: 7351 - 7400 of 31059 results

  1. pSucCD2

    Plasmid
    #83324
    Purpose
    expresses E. coli succinyl-CoA synthetase
    Depositor
    Robert Hausinger
    Insert
    sucCD
    Expression
    Bacterial
    Available Since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    83324-plasmid-map-sequence-id-160476

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  4. JMC044-B12

    Plasmid
    #58752
    Purpose
    bacterial expression of PDB 4NW3
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4NW3 (KMT2A Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available Since
    Aug. 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. RS35075_pMQ30_KO_Kan

    Plasmid
    #185390
    Purpose
    Non-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11
    Depositor
    Adam Arkin
    Article
    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1
    Inserts
    RR42_RS35075 upstream flanking homology region
    RR42_RS35075 downstream flanking homology region
    nptII kanamycin resistance marker
    Expression
    Bacterial
    Available Since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    185390-plasmid-map-sequence-id-371206

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  7. UBC80

    Plasmid
    #25267
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubc80.229.304 (TSG101 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. pTSara-C

    Plasmid
    #60722
    Purpose
    Expresses the C-terminal fragment of split T7 RNAP split a position 179. Insert is driven by the arabinose inducible promoter PBAD. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    Residues 180-880 of T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Only amino acids 180-880 of T7 RNAP
    Promoter
    PBAD
    Available Since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60722-plasmid-map-sequence-id-97708

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  11. pTSara-N

    Plasmid
    #60721
    Purpose
    Expresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    Residues 1-179 of T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Only amino acids 1-179 of T7 RNAP
    Promoter
    PBAD
    Available Since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60721-plasmid-map-sequence-id-97707

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  12. Dead Streptavidin-SpyTag

    Plasmid
    #59548
    Purpose
    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead Streptavidin-SpyTag
    Tags
    SpyTag
    Expression
    Bacterial
    Mutation
    The construct lacks final K of SpyTag
    Promoter
    T7
    Available Since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. 4RCI

    Plasmid
    #64653
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    YTHDF1:JMC02A-E04:C231829 (YTHDF1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    April 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pBbB8k-csg-amylase

    Plasmid
    #166859
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469
    Insert
    csgBACEFG, with α-amylase fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    April 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    166859-plasmid-map-sequence-id-329647

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  17. pB2Hopt

    Plasmid
    #163830
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available Since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163830-plasmid-map-sequence-id-337586

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  28. pTJV1Sc3-rpoB

    Plasmid
    #166983
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    RecT (recT E. coli)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    pVvanCC
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166983-plasmid-map-sequence-id-331334

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  29. 6xHis-MBP-huPb2Cas12a

    Plasmid
    #174686
    Purpose
    Expresses 6xHis-MBP tagged Pb2Cas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huPb2Cas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174686-plasmid-map-sequence-id-346201

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  31. pBCG10

    Plasmid
    #232323
    Purpose
    Make mazF a counterselection marker to replace sacB in pZP06C
    Depositor
    Chenggang Wu
    Insert
    mazF-tetR
    Expression
    Bacterial
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232323-plasmid-map-sequence-id-462282

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  32. PCYT2A

    Plasmid
    #42415
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PCYT2A (PCYT2 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  37. INPP5EA

    Plasmid
    #42478
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    INPP5EA (INPP5E Human)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  44. pGEX6P1-DEST-Strep2

    Plasmid
    #119753
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and Strep-tag II fusion tag
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    May 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119753-plasmid-map-sequence-id-243311

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  47. pETcLIC-GFP

    Plasmid
    #183514
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    June 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    183514-plasmid-map-sequence-id-362488

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Showing: 7351 - 7400 of 31059 results