32,069 results
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Plasmid#126597Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only
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pEvol-NnSULT1C1-cysDNCQ
Plasmid#188983Purposebiosynthesize sulfotyrosine in E. coli.DepositorInsertsNnSULT1C1
cysD
cysN
cysC
cysQ
ExpressionBacterialAvailable SinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
OmpF
Plasmid#213135PurposeExpresses outer membrane protein FDepositorAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSP3535
Plasmid#46886PurposeGram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible ExpressionDepositorTypeEmpty backboneUseNisin-inducible expression; gram-positive bacteri…ExpressionBacterialPromoterPnisAAvailable SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBbE1c-RFP
Plasmid#35332DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterTrcAvailable SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
NUDT22 (NUDT22A-c001)
Plasmid#98230PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NR1I2_Hormone-recep
Plasmid#110020PurposeProtein expression and purification of NR1I2_Hormone-recepDepositorInsertNR1I2_Hormone-recep (NR1I2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RXRA_Hormone-recep
Plasmid#109850PurposeProtein expression and purification of RXRA_Hormone-recepDepositorInsertRXRA_Hormone-recep (RXRA Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S
Plasmid#60815PurposedCas9 with single cysteine residue (M1C) for site-specific labellingDepositorInsertdCas9 M1C D10A C80S H840A C574S
TagsHis10 and MBPExpressionBacterialMutationM1C D10A C80S H840A C574SAvailable SinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSETB/mStayGold(c4)
Plasmid#212018PurposeFor N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.DepositorInsertmStayGold(c4)
ExpressionBacterialPromoterT7Available SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pProUSER13F4C
Plasmid#178023PurposeE. coli - B. subtilis nicking vector pProUSER13F4C. Ap-BBR1; ΔsigF-insertion-site; Km; manR-PmanADepositorInsertnicking site to be replaced with your gene of interest
ExpressionBacterialPromoterPmanAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pProUSER13E3F
Plasmid#178026PurposeE. coli - B. subtilis nicking vector pProUSER13E3F. Ap-BBR1; yhgE-insertion-site; Spc; tetR-PtetDepositorInsertnicking site to be replaced with your gene of interest
ExpressionBacterialPromoterPtetAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pProUSER13G2F
Plasmid#178027PurposeE. coli - B. subtilis nicking vector pProUSER13G2F . Ap-BBR1; rsbP-insertion-site; Ery; tetR-PtetDepositorInsertnicking site to be replaced with your gene of interest
ExpressionBacterialPromoterPtetAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-rIgG-Fc2-MFSD8(L9)-Zeo
Plasmid#239947PurposeExpression of MFSD8 loop 9 tagged with rabbit IgG Fc2DepositorInsertFc2-MFSD8(L9)
ExpressionBacterial and MammalianAvailable SinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RANGAP1-CTD
Plasmid#228947Purposeexpress the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimizedDepositorInsertRANGAP1-CTD (RANGAP1 Human)
TagsHis tag and thrombine cleavage site : MGSSHHHHHHS…ExpressionBacterialMutationcontains aa 418-587 onlyPromoterT7-lacAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)
Plasmid#205049PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)
TagsMGHHHHHGGAExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
PRPF4.001.169
Plasmid#137233PurposeExpresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.DepositorAvailable SinceNov. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-PLtetO1-tetR-T500
Plasmid#45391DepositorInserttet repressor
ExpressionBacterialPromoterPL-tetO1Available SinceJune 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCas3cRh
Plasmid#133773PurposeExpresses all necessary components of Type I-C CRISPR-Cas systemDepositorAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
ILF2_DZF_pGEX
Plasmid#135162PurposeRecombinant expression of ILF2 with dual-affinity tagDepositorAvailable SinceNov. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH6-MBP-TEV-BirA
Plasmid#179694PurposeExpresses His6 tagged MBP-BirA fusion proteinDepositorInsertBirA
TagsHis6-MBPExpressionBacterialAvailable SinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-Impact-T4gp41
Plasmid#228207PurposeExpression in BL21(DE3) of T4 helicaseDepositorInsert41 (41 Escherichia phage T4)
TagsSce VMA intein-chitin binding domainExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-Impact-T4gp59
Plasmid#228206PurposeExpression in BL21(DE3) of WT T4 helicase loaderDepositorInsert59 (59 Escherichia phage T4)
TagsSce VMA intein-chitin binding domainExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFREE
Plasmid#92050PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRExpressionBacterialPromoterpRhamBAD and ptetAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
PS6K-mcherry-iATPsnFR1.1
Plasmid#227238PurposeInducible ATP sensor for E. coliDepositorInsertiATPsnFR1.1
TagsmCherryExpressionBacterialAvailable SinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-mCherry-MED1-IDR
Plasmid#194545PurposeBacterial Expression of 6xHis mCherry-MED1-IDR Fusion ProteinDepositorAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(M1-42)
Plasmid#71875PurposeExpresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cellsDepositorAvailable SinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
GPX4
Plasmid#38797PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only