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Bacterial Expression

29,712 results

Showing: 2601 - 2650 of 29712 results

  6. pTP1174

    Plasmid
    #104162
    Purpose
    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
    Use
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104162-plasmid-map-sequence-id-200079

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  8. pPEPY-PF6-lacI

    Plasmid
    #85589
    Purpose
    Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae
    Depositor
    Jan-Willem Veening
    Article
    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do
    Insert
    lacI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PF6
    Available since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    85589-plasmid-map-sequence-id-172946

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  11. pET_RTX-6xHis

    Plasmid
    #145029
    Purpose
    pET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tag
    Depositor
    Andrew Ellington
    Article
    Bhadra et al BioProtocol 2021
    Insert
    RTX
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145029-plasmid-map-sequence-id-292639

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  13. pJCX3-ACTG2_R257C

    Plasmid
    #228911
    Purpose
    Expresses mutant R257C smooth muscle actin gamma-2 in human cells
    Depositor
    Roberto Dominguez
    Insert
    Actin gamma 2, smooth muscle (ACTG2 Human)
    Use
    Tags
    HIS-FLAG-TEV
    Expression
    Bacterial and Mammalian
    Mutation
    R257C
    Promoter
    CMV
    Available since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    228911-plasmid-map-sequence-id-457665

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  14. pJCX3-ACTG2_WT

    Plasmid
    #228910
    Purpose
    Expresses wildtype smooth muscle actin gamma-2 in human cells
    Depositor
    Roberto Dominguez
    Insert
    Actin gamma 2, smooth muscle (ACTG2 Human)
    Use
    Tags
    HIS-FLAG-TEV
    Expression
    Bacterial and Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    228910-plasmid-map-sequence-id-457664

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  17. pJA48 HLA-B*57:01

    Plasmid
    #180444
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*57:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    180444-plasmid-map-sequence-id-366471

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  18. pET22b-T5-sfGFP*-XcP4H-MH4R

    Plasmid
    #178043
    Purpose
    Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP
    Depositor
    Han Xiao
    Article
    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1
    Inserts
    superfolder green fluorescent protein with TAG at 151 position
    XcP4H with W179F mutation
    dihydropterine reductase from human
    pterin-4a-carbinolamine dehydratase from human
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    178043-plasmid-map-sequence-id-359586

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  19. pORTMAGE-2

    Plasmid
    #72677
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    pL promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    72677-plasmid-map-sequence-id-457485

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  20. pSAP

    Plasmid
    #206429
    Purpose
    A vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    206429-plasmid-map-sequence-id-419733

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  22. pGEXTK-Pak1 70-117

    Plasmid
    #12217
    Depositor
    Jonathan Chernoff
    Insert
    Pak1 (PAK1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    PBD (p21-binding domain), aa 70-117
    Promoter
    Available since
    Aug. 11, 2006
    Availability
    Academic Institutions and Nonprofits only
    12217-plasmid-map-sequence-id-166258

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  26. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3

    Plasmid
    #189755
    Purpose
    Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis
    Depositor
    Dorus Gadella
    Article
    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi
    Insert
    mTurquoise2_spatial-linker-P2A_mScarlet-I3
    Use
    Tags
    6xHis
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV & Rha
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    189755-plasmid-map-sequence-id-385774

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  28. CD Ugi1

    Plasmid
    #177194
    Purpose
    Greengate entry vector pGGC000 modified to contain bladderwort insulator 1
    Depositor
    Wayne Parrott
    Article
    Park et al bioRxiv 2021.11.04.467280
    Insert
    Utricularia gibba insulator 1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 28, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    177194-plasmid-map-sequence-id-361543

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  33. esmGFP-C10-pVTCB052

    Plasmid
    #231303
    Purpose
    Plasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coli
    Depositor
    Alexander Rives
    Insert
    esmGFP-C10
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231303-plasmid-map-sequence-id-463619

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  38. pCgCas9_recT

    Plasmid
    #153337
    Purpose
    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153337-plasmid-map-sequence-id-305977

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  40. pTCF5 HLA-A*01:01

    Plasmid
    #180448
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*01:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    180448-plasmid-map-sequence-id-355109

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  44. pETM33_Nsp15

    Plasmid
    #156481
    Purpose
    Bacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp15 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156481-plasmid-map-sequence-id-307733

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  45. pCas9(D10A)

    Plasmid
    #74495
    Purpose
    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    Cas9 D10A
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    D10A
    Promoter
    pTeto
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74495-plasmid-map-sequence-id-140495

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  46. pTCF37 HLA-B*35:01

    Plasmid
    #180450
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*35:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    180450-plasmid-map-sequence-id-358091

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  50. pTP943

    Plasmid
    #104157
    Purpose
    Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse IgG1 Fab specific nanobody TP886
    Use
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104157-plasmid-map-sequence-id-200077

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Showing: 2601 - 2650 of 29712 results