31,059 results
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Plasmid#196472PurposeProtein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcherDepositorInsertBCB
ExpressionBacterialPromoterT7Available SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-mECitrine
Plasmid#105151PurposeYeast gene targetingDepositorInsertkanMX6-P3nmt1-mECitrine
Tags3nmt1-mECitrineExpressionBacterial and YeastMutationF64L, Q69M, A206KPromoter3nmt1Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-mECitrine
Plasmid#105153PurposeYeast gene targetingDepositorInsertkanMX6-P81nmt1-mECitrine
Tags81nmt1-mECitrineExpressionBacterial and YeastMutationF64L, Q69M, A206KPromoter81nmt1Available SinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-mECitrine
Plasmid#105152PurposeYeast gene targetingDepositorInsertkanMX6-P41nmt1-mECitrine
Tags41nmt1-mECitrineExpressionBacterial and YeastMutationF64L, Q69M, A206KPromoter41nmt1Available SinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
RC(WW)-LgBit
Plasmid#208931PurposeRecognition domain for in vitro MAPK-activity sensorDepositorAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT11.G
Plasmid#200999PurposepLv1-pUC18R6KT-miniTn7-Gm-sfGFP, GmR, ApRDepositorInsertsuperfolder GFP
ExpressionBacterialPromoterJ23104Available SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-T4gp43(WT)
Plasmid#228203PurposeExpression in BL21(DE3) of WT T4 DNA polymeraseDepositorInsert43
TagsSce VMA intein-chitin binding domainExpressionBacterialPromoterT7Available SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDF-mTET1
Plasmid#81052Purposebacterial expression of murine TET1DepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD miniSOG
Plasmid#91774PurposeBacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)DepositorInsertmini Singlet Oxygen Generator
TagsHexa-Histidine tagExpressionBacterialPromoteraraBADAvailable SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a hMDH1v3 (+TEV)
Plasmid#204229PurposeBacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).InserthMDH1v3 (MDH1 Human)
Tags6X HisExpressionBacterialMutationInserted a G after ATG to maintain kozak sitePromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2G1
Plasmid#15790DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMC037-HisMS2_PLP_pac
Plasmid#128233PurposeExpression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.DepositorInsertsMaturation Protein
Coat Protein Dimer
ExpressionBacterialPromoterT7Available SinceNov. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCBFD1/HBFD1Bad
Plasmid#53364PurposeWill convert beta-carotene into astaxanthin in E. coli when expression of the hbfd1 cDNA is induced with arabinose. Use with pAC-BETA or pAC-BETAipi.DepositorInsertcbfd1
ExpressionBacterialPromoterlacAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-Geo_st
Plasmid#87700PurposeExpression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBPDepositorInsertGeoCas9
Tags10xHis-MBP-TEVExpressionBacterialAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
(+)-Limonene Synthase
Plasmid#112230Purpose(+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.DepositorInsert(+)-Limonene Synthase
TagsN-terminal His-tagExpressionBacterialMutationdeleted amino acids 1-52Available SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDB364
Plasmid#110275PurposeN-terminal 10-His DsbC TEV-peptide fusionDepositorTypeEmpty backboneTags10-His, TEV cleavage peptide, and mature DsbC (i.…ExpressionBacterialPromoterT7Available SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pExp-Sumo
Plasmid#112574PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8His and Strep tagExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 V416I
Plasmid#81045Purposemammalian expression of LOV V416IDepositorInsertLOV V416I
TagsmCherryExpressionBacterial, Insect, and Mamm…MutationV416IAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-Optimized UBE1L
Plasmid#165099Purposeecoli optimized expression of GST-fusion of human Ube1LDepositorInsertUbeE1L (UBA7 Human)
TagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expressionAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFCcGi
Plasmid#59324PurposeInducible expression of GFP and constitutive expression of mCherryDepositorInsertsmCherry
EGFP
ExpressionBacterialPromoterPbad and rpsMAvailable SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196)-Strep
Plasmid#196208PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196)-Strep.DepositorAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Profiline
Plasmid#89925PurposeBinds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actinDepositorAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUdO2c
Plasmid#210231Purposemedium-copy plasmid for bacterial expressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTcI42-Wasabi
Plasmid#86118PurposemWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42DepositorInsertsTcI42
mWasabi
Tags6xHisExpressionBacterialMutationA67T, K6N, S33T, Y61H, L119P, F122CPromoterpR/pL and pR/pL, LacIAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-PfuX7
Plasmid#182364PurposeExpresses PfuX7 DNA polymeraseDepositorInsertPfuX7 DNA polymerase
TagsHis-tagExpressionBacterialMutationV93QPromoterT7Available SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgRNAtet-lvaA
Plasmid#106397PurposeGuide RNA plasmid targeting lvaA on BBR1-UP originDepositorInsertsgRNA towards lvaA in Pseudomonas putida
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI
Plasmid#115211PurposeExpresses PsmCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPsmCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-GGA3-VHS-GAT
Plasmid#173719PurposeVHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.DepositorAvailable SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry VPS37b
Plasmid#245030PurposeProtein localization of human wild-type VPS37b in cellsDepositorAvailable SinceSept. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFC
Plasmid#214950PurposeBacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation.DepositorInsertsVNp-anti-Lysozyme Fab heavy chain amino-BiFC fusion
VNp-anti-Lysozyme Fab light chain amino-BiFC fusion
Tags6xHis, Amino half of venus BIFC fragment, Carboxy…ExpressionBacterialPromoterT7Available SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1 (1-366)
Plasmid#193039PurposeBacterial expression plasmid for full-length IST1 (1-366).DepositorAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Stx3
Plasmid#186685PurposeExpresses Syntaxin 3 in E. ColiDepositorInsertSyntaxin 3
Tags6 his tagExpressionBacterialAvailable SinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP1B 1-199
Plasmid#115326PurposeExpresses residues 1-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-24DepositorAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-mDyn3 pcDNA3
Plasmid#36265DepositorAvailable SinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSB219
Plasmid#164125Purposep15A backbone with PhlF binding sitesDepositorInsertsfGFP
ExpressionBacterialPromoterJ23105Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-FRT
Plasmid#113398Purposeexpresses gRNA for Cas9 FRT targettingDepositorInsertexo, beta, gam, sgRNA-FRT
UseCRISPRExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN1
Plasmid#180282PurposeCas9 cassette (TU1)DepositorInsertConLS - pPGK1 - Cas9 - tPGK1 - ConR1
ExpressionBacterialPromoterpPGK1Available SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG014 (RADAR)
Plasmid#157892PurposeExpresses the RADAR defense systemDepositorInsertRADAR
ExpressionBacterialAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-AMSH*
Plasmid#66712PurposeExpresses activated AMSH in E. coliDepositorAvailable SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a hMDH2
Plasmid#204230PurposeBacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).InserthMDH2 (MDH2 Human)
Tags6X HisExpressionBacterialMutationDoes not include transit peptide and includes a M…PromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
SuperNova/pRSETB
Plasmid#53234Purposemonomeric photosensitizing fluorescent protein for chromophore-assisted light inactivationDepositorInsertmonomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
Tags6xHis, T7 tag, and Xpress tagExpressionBacterialAvailable SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAK405
Plasmid#37114DepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET32a-HD46Q
Plasmid#11515DepositorInserthuntingtin, exon 1, 46Q glutamines (HTT Human)
TagsHis, His (out of frame), and TrxExpressionBacterialMutationhuntingtin exon 1 with 46 glutamines, vector m…Available SinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
His6-SUMO-pET28a-EfaCas1
Plasmid#112793PurposeTo express enterococcus faecalis Cas1 protein in E. coliDepositorInsertEfaCas1
TagsHis6-SUMOExpressionBacterialAvailable SinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-miRFP670
Plasmid#79984PurposeMonomeric near-infrared fluorescent protein miRFP670 in bacterial plasmidDepositorInsertmiRFP670
ExpressionBacterialPromoteraraBAvailable SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-JNK1a1
Plasmid#47574PurposeBacterial expression plasmid for GST-3xFlag-tagged JNK1a1DepositorAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
SRC_HUMAN_D0
Plasmid#79700PurposeThis plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
AARS_C2_pNIC-Bio3
Plasmid#153047PurposeExpression construct for producing Avi tagged Alanyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAR357_SFP_pCDF-1b
Plasmid#122851PurposeCo-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.DepositorInsertSfp
ExpressionBacterialPromoterT7 promoterAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-hPXR-LBD
Plasmid#67769PurposeExpression GST-hPXR-LBD in bacteriaDepositorInserthuman pregnane X receptor (PXR) ligand binding domain (NR1I2 Human)
TagsGSTExpressionBacterialMutationContains amino acids 107 to 434 of human PXRPromoterTacAvailable SinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only