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Bacterial Expression

29,729 results

Showing: 4251 - 4300 of 29729 results

  1. pJYS1Peftu

    Plasmid
    #85546
    Purpose
    Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter
    Depositor
    Sheng Yang
    Article
    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.
    Inserts
    Cpf1
    recT
    Use
    CRISPR; Shuttle vector corynebacterium glutamicum…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    etfu and unknown
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    85546-plasmid-map-sequence-id-176968

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  3. pTSlb-N748D

    Plasmid
    #60732
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60732-plasmid-map-sequence-id-99421

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  7. PTPRB

    Plasmid
    #39165
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRB (PTPRB Human)
    Use
    Tags
    GST-PreScission
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. pComb3HSS

    Plasmid
    #64133
    Purpose
    2nd generation plasmid for phage display on modified geneIII, contains stuffer fragment
    Depositor
    Carlos Barbas
    Article
    Barbas et al Methods, A companion to Methods in Enzym
    Inserts
    SS Stuffer
    Light Chain Stuffer
    Heavy Chain Stuffer
    Use
    Phage display
    Tags
    geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64133-plasmid-map-sequence-id-119143

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  9. pJJS012

    Plasmid
    #188335
    Purpose
    PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    Linker::mTagBFP2::mIAA7
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188335-plasmid-map-sequence-id-370742

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  10. pYTK080

    Plasmid
    #65187
    Purpose
    Encodes ZeocinR as a Type 6 part to be used in the Dueber YTK system
    Depositor
    John Dueber
    Article
    Lee et al ACS Synth Biol. 2015 Apr 14.
    Insert
    ZeocinR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    65187-plasmid-map-sequence-id-110240

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  16. pSLC-284

    Plasmid
    #124294
    Purpose
    A low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.
    Depositor
    Swaine Chen
    Article
    Eshaghi et al Pathogens. 2016 Jan 5;5(1). pii: pathoge
    Insert
    vsfGFP-9
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    124294-plasmid-map-sequence-id-251247

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  21. SRPK1 (SRPK1A-c100)

    Plasmid
    #98246
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SRPK1A (SRPK1 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    98246-plasmid-map-sequence-id-193287

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  22. SPLICSFAST-LDh-LY-P2A

    Plasmid
    #214895
    Purpose
    Expresses equimolar Lipid Droplet- and Lysosome- targeted split-FAST fragments for Short-range LD-LY Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-LDh-LY-P2A
    Use
    Tags
    none
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214895-plasmid-map-sequence-id-423481

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  23. pET-Sangamo-HIS

    Plasmid
    #40787
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    C Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus,Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40787-plasmid-map-sequence-id-68902

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  25. pBad-mAmetrine1.1

    Plasmid
    #18084
    Purpose
    Bacterial expression of mAmetrine (version 1.1) fluorescent protein
    Depositor
    Robert Campbell
    Article
    Ai et al Nat Methods. 2008 May . 5(5):401-3.
    Insert
    mAmetrine1.1
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    The fluorescent protein was inserted between XhoI…
    Promoter
    Available since
    May 29, 2008
    Availability
    Academic Institutions and Nonprofits only

  28. RHOAA-c033

    Plasmid
    #205116
    Purpose
    RHOA protein expression
    Depositor
    Paul Brennan
    Insert
    RHOA (RHOA Human)
    Use
    Tags
    6His-TEV
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205116-plasmid-map-sequence-id-411206

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  30. pAD-LyseR (in lacZ deficient strain)

    Plasmid
    #99245
    Purpose
    For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)
    Depositor
    Jeff Hasty
    Article
    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102
    Insert
    phage lambda gene R (R phage lambda)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    beta-lactamase promoter
    Available since
    Oct. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99245-plasmid-map-sequence-id-191039

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  36. p1A

    Plasmid
    #162692
    Purpose
    Expresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    H. neapolitanus CbbLS and S. elongatus Prk
    Use
    Tags
    N terminal 6x His tag on PRK
    Expression
    Bacterial
    Mutation
    Promoter
    PLtet0-1 promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162692-plasmid-map-sequence-id-324151

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  42. pTPH413

    Plasmid
    #185728
    Purpose
    Bacterial PAM profiling of adenine base editor variants
    Depositor
    David Liu
    Article
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Inserts
    ecTadA(8e)-neNme2-C
    Nme2Cas9 sgRNA
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…
    Promoter
    Available since
    Sept. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185728-plasmid-map-sequence-id-364088

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  43. pBAD-pHScarlet

    Plasmid
    #191258
    Purpose
    Bacterial expression plasmids for pH sensitive fluorescent protein pHScarlet
    Depositor
    Robert Campbell
    Insert
    pHScarlet
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    191258-plasmid-map-sequence-id-374867

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  44. GST-PBD

    Plasmid
    #60880
    Purpose
    Bacterially expressed GST-tagged human polo-like kinase 1 PBD AA365-653
    Depositor
    Trina Schroer
    Article
    Yeh et al EMBO J. 2013 Apr 3;32(7):1023-35. doi: 1
    Insert
    Polo-like kinase 1 (PLK1 BC01486, Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 4251 - 4300 of 29729 results