32,656 results
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Plasmid#182419PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusionDepositorInsertVNp-mNeongreen-stefin (CSTA Human)
TagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-pheS
Plasmid#111611PurposeYeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.DepositorInsertpheS
ExpressionBacterialAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-B-iRFP
Plasmid#31855Purposea phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imagingDepositorInsertiRFP
ExpressionBacterialMutationS13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D2…PromoteraraBADAvailable SinceSept. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 YAP1 WW domain #2
Plasmid#104228PurposeBacterial expression of WW domain #2 from YAP1DepositorInsertYAP1 WW domain #2 (YAP1 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 YAP1 WW domain #1
Plasmid#104227PurposeBacterial expression of WW domain #1 from YAP1DepositorInsertYAP1 WW domain #1 (YAP1 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7FTrp)RS
Plasmid#177310PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA3b67Rb
Plasmid#58311PurposeShuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.DepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
MPRA Empty Vector
Plasmid#204865PurposeVector used for high-throughput cloning and subsequent transfection of MPRA libraries into mammalian cells.DepositorTypeEmpty backboneExpressionBacterial and MammalianAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAF-MutS
Plasmid#86605PurposeE. coli MutS expression plasmidDepositorInsertE. coli MutS (D835R,R840E)
Tagshis-tag and ald-tagExpressionBacterialMutationD835R, R840EPromoterT7Available SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTE102
Plasmid#59397PurposeMethyloBrick expression vector, mxaF promoter; TcRDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPmxaFAvailable SinceDec. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-CLIP-tag
Plasmid#167274PurposeOverexpression of CLIP-tag in E. coliDepositorInsertCLIP-tag
TagsHis x10ExpressionBacterialPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCA1.332 pDUOTK1-L1
Plasmid#162351PurposeLevel 0 terminator acceptor containing dual fluorophores for testing transcription terminator efficiencyDepositorInsertseYFP
LacZ alpha
mTagBFP
UseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
p359_DG
Plasmid#234702PurposeGolden gate domesticated pET28b for scarless cloning with His10-mNeongreen-3CDepositorTypeEmpty backboneTagsHis10-mNeongreen-3CExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAH01Sp::Cas9
Plasmid#128160PurposePtet-controlled expression of S. pyogenes Cas9DepositorInsertSp Cas9
ExpressionBacterialPromoterPtetAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_NAP1L1-Halo
Plasmid#175342PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST-AUP1 379-410:Ube2G2
Plasmid#185335PurposeUsed for co-expression of the AUP1 G2BR with UBE2G2 for crystallographyDepositorAvailable SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBRC901
Plasmid#167948Purposeexpress wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)DepositorInsertwild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
Tagshexa-histidine tagExpressionBacterialPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVH015
Plasmid#80398PurposeContains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blotsDepositorUseSynthetic BiologyTagsC-terminal 3xFLAG tag and C-terminal LZx domainExpressionBacterialAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKB01_tagRFP
Plasmid#69103PurposeExpresses tagRFP (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.DepositorInserttagRFP
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDiGi
Plasmid#59323PurposeInducible expression of dsRed and GFP in bacteriaDepositorInsertsEGFP
dsRed
ExpressionBacterialPromoterPbad and PlacAvailable SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2A
Plasmid#15780DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
EcMscL
Plasmid#92418PurposeE. coli Mechanosensitive Channel of Large ConductanceDepositorInsertMscL (mscL E. coli)
Tags6x Histidine Tag and Thrombin Cleavage Site betwe…ExpressionBacterialPromoterT7 PromoterAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMflT-o4
Plasmid#101312PurposeContains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.DepositorInsertoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG004 (RT-Abi-P2)
Plasmid#157882PurposeExpresses the RT-Abi-P2 defense systemDepositorInsertRT-Abi-P2
ExpressionBacterialAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pet26-MBP-ELIC
Plasmid#39239DepositorInsertEriwinia chrysanthemi - pentameric ligand gated ion channel
TagsHis-10 and MBPExpressionBacterialPromoterT7Available SinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pECJ3
Plasmid#75465PurposeToggle switch plasmid containing lacI-pdt3DepositorInsertlacI-pdt3
ExpressionBacterialPromoterpLtetOAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_RAN-Halo
Plasmid#175339PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pES001 MBP-GSK3β-HA-His
Plasmid#154010PurposeExpresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coliDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2T
Plasmid#15808DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pR6K-GFP-CASTIF
Plasmid#199653PurposeR6K plasmid encoding a type I-F CAST but no CRISPR arrayDepositorInsertCAST I-F systems
ExpressionBacterialPromoterJ23119 promoterAvailable SinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPP5371
Plasmid#128732PurposeGateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal Cya tagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE143
Plasmid#118495Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsYFP2
ExpressionBacterialAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTec_mCherry
Plasmid#229145PurposePromoter strength evaluation of PtecDepositorInsertmCherry
ExpressionBacterialMutationNoPromoterPtecAvailable SinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBR BsmAIM
Plasmid#206206PurposeConstitutive expression of BsmAIM methyltransferaseDepositorInsertBsamAIM
ExpressionBacterialAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCgsgRNA_crtYf
Plasmid#153338PurposeDouble-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; SpcrDepositorInsertcrRNA targeting crtYf
UseCRISPRExpressionBacterialAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCre-SacB-zeo
Plasmid#107706PurposeExpresses Cre recombinase for excising chromosomal loxP-Hyg-loxP cassette inserts.DepositorInsertble (Zeo resistance)
ExpressionBacterialAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJS169
Plasmid#48882PurposeNegative feedback plasmid from oscillatorDepositorInsertlacI with ssrA tag
UseSynthetic BiologyTagsssrA (TSAANDENYALAA)ExpressionBacterialPromoterPara/lacAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET mOrange LIC cloning vector (u-mOrange)
Plasmid#29770DepositorTypeEmpty backboneTagsmOrangeExpressionBacterialAvailable SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJS167
Plasmid#48881PurposePositive feedback plasmid from oscillatorDepositorInsertsaraC gene with ssrA tag
monomeric yeast-enhanced green fluorescent protein
UseSynthetic BiologyTagsssrA (TSAANDENYALAA)ExpressionBacterialPromoterPara/lacAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28_WT-LGP2 (codon optimized)
Plasmid#248163PurposeExpresses full-length human LGP2 in E. coliDepositorAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_5F66
Plasmid#234226PurposeHeterologous protein expression of peptidyl-prolyl cis-trans isomerase A (PPIase A) in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Dox-SOScat
Plasmid#207329PurposePiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)DepositorInsertson of sevenless homolog 2 variant, catalytic domain
Tagsmyristoylation tag - BFPExpressionBacterialMutationrange: 593 to 1078PromoterTet Response ElementAvailable SinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_YWHAZ_14-3-3
Plasmid#109808PurposeProtein expression and purification of YWHAZ_14-3-3DepositorInsertYWHAZ_14-3-3 (YWHAZ Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMW41: pET-His-MBP-SUMO-R2Bm-SII
Plasmid#209290PurposeExpresses the R2Bm ORF in E. coliDepositorInsertR2
TagsHis, MBP, StrepII, and bdSUMOExpressionBacterialPromoterT7Available SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRiboI-Sec-APEX2m
Plasmid#176845PurposeExpression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)DepositorInsertpRibo-Sec-APEX2m
ExpressionBacterialPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_6IYE
Plasmid#234237PurposeHeterologous protein expression of peptidyl-tRNA hydrolase (PTH) in Escherichia coliDepositorInsert6IYE (pth Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841)
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
TDP43-NTD
Plasmid#241024PurposeBacterial expression of TDP-43 NTDDepositorInsertTDP43-NTD
Tags6x HisExpressionBacterialMutationchanged N81 to stop codonAvailable SinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIV p24
Plasmid#212195PurposeBacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminusDepositorInsertChain A, Capsid protein p24, HIV type 1
TagsPolyhistidineExpressionBacterialAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP
Plasmid#166439PurposeBacterial expression of 6x His tagged mEGFPDepositorInsertmEGFP
ExpressionBacterialAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only