Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

Your Selections

Bacterial Expression

29,729 results

Showing: 4551 - 4600 of 29729 results

  6. EGFP-p300 (D1399Y)

    Plasmid
    #191763
    Purpose
    Expression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)
    Depositor
    Daniel Gerlich
    Article
    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:
    Insert
    FRB-EGFP-p300 core (D1399Y) (EP300 Human)
    Use
    Tags
    EGFP (N-terminal of p300 core (D1399Y)) and FRB (…
    Expression
    Bacterial and Mammalian
    Mutation
    catalytic D1399Y mutation in p300
    Promoter
    EF1alpha
    Available since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    191763-plasmid-map-sequence-id-383798

    Enlarge

  17. PNMT

    Plasmid
    #64078
    Purpose
    Expression of human Phenylethanolamine N-methyltransferase
    Depositor
    Vern Schramm
    Article
    Stratton et al ACS Chem Biol. 2017 Feb 17;12(2):342-346
    Insert
    Phenylethanolamine N-methyltransferase (PNMT Human, Synthetic)
    Use
    Tags
    Histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. pGJJ045

    Plasmid
    #183621
    Purpose
    abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.
    Depositor
    Ben Lehner
    Article
    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    183621-plasmid-map-sequence-id-361478

    Enlarge

  20. pJMP3654

    Plasmid
    #222349
    Purpose
    Expression vector with GFP expressed under PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli.
    Depositor
    Jason Peters
    Insert
    sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PabstBR
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222349-plasmid-map-sequence-id-439084

    Enlarge

  22. pFC902

    Plasmid
    #106904
    Purpose
    The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al Fungal Genet Biol. 2018 Jan 8. pii: S108
    Inserts
    cas9
    pyrG
    U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106904-plasmid-map-sequence-id-206325

    Enlarge

  23. CYP2C9 in vector pCW ori+

    Plasmid
    #69554
    Purpose
    Modification of CYP2C9 and 2C19 s for bacterial expression
    Depositor
    Joyce Goldstein
    Insert
    CYP2C9 (CYP2C9 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69554-plasmid-map-sequence-id-287980

    Enlarge

  25. pBRG2

    Plasmid
    #149474
    Purpose
    Broad-Host range expression vector, pBBR origin, RhaR-RhaS activator, PrhaBAD promoter, GFPmut3b, Kanamycin Resistance Marker.
    Depositor
    Christopher Reisch
    Article
    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629
    Insert
    RhaS-RhaR-GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaBAD
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    149474-plasmid-map-sequence-id-313276

    Enlarge

  28. pH6-TEV-mCherry

    Plasmid
    #132430
    Purpose
    Expresses His6-tagged mCherry in E. coli using T7 promoter
    Depositor
    Mark Arbing
    Insert
    mCherry
    Use
    Tags
    N-terminal His6 tag with TEV protease site
    Expression
    Bacterial
    Mutation
    Codon optimized
    Promoter
    T7
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    132430-plasmid-map-sequence-id-258769

    Enlarge

  31. pNDGG004

    Plasmid
    #231317
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Tc-par-ELT4; GG assembly from pOGG011, pOGG012, pOGG014, pOGG042, and pOGG004, TcR
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231317-plasmid-map-sequence-id-463766

    Enlarge

  32. pNDGG051

    Plasmid
    #231320
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Sp-par-ELT4; GG assembly from pOGG004, pNDGG002, pOGG011, pOGG012, and pOGG014, SpR
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231320-plasmid-map-sequence-id-463765

    Enlarge

  33. pNDGG003

    Plasmid
    #231316
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Tc-par-ELT4; GG assembly from pOGG010, pOGG012, pOGG014, pOGG042, and pOGG004, TcR
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231316-plasmid-map-sequence-id-463764

    Enlarge

  34. pNDGG053

    Plasmid
    #231322
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG011, pOGG012, and pOGG014, NmR/KmR
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231322-plasmid-map-sequence-id-462969

    Enlarge

  39. pRMC2-tat:msfGFP

    Plasmid
    #194915
    Purpose
    Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194915-plasmid-map-sequence-id-384626

    Enlarge

  40. pCgsgRNA_crtYf

    Plasmid
    #153338
    Purpose
    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19
    Insert
    crRNA targeting crtYf
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153338-plasmid-map-sequence-id-306018

    Enlarge

  42. pDUAL_2_araBAD

    Plasmid
    #155176
    Purpose
    see pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155176-plasmid-map-sequence-id-310563

    Enlarge

Showing: 4551 - 4600 of 29729 results