Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

Your Selections

Insect Expression

4,932 results

Showing: 401 - 440 of 4932 results

  1. pOCC102-His-MBP-DDX3X(WT)

    Plasmid
    #219988
    Purpose
    DDX3X(WT) protein purification
    Depositor
    Simon Alberti
    Insert
    DDX3X(WT) (DDX3X Human)
    Tags
    6xHis and MBP
    Expression
    Insect
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219988-plasmid-map-sequence-id-436790

    Enlarge

  3. pCFD5-NS

    Plasmid
    #149546
    Purpose
    Expression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.
    Depositor
    Norbert Perrimon
    Article
    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Insect
    Promoter
    U6
    Available Since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    149546-plasmid-map-sequence-id-306364

    Enlarge

  5. pSR660

    Plasmid
    #65216
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-8 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    P10 and polyhedrin
    Available Since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    65216-plasmid-map-sequence-id-115626

    Enlarge

  11. pOCC5

    Plasmid
    #118863
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118863-plasmid-map-sequence-id-232276

    Enlarge

  12. DDB1

    Plasmid
    #25246
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DDB1_1 (DDB1 Human)
    Use
    Baculovirus expression
    Tags
    His
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25246-plasmid-map-sequence-id-275348

    Enlarge

  15. pExpreS2-1-C3-PA-10H-SIII

    Plasmid
    #175443
    Purpose
    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Tags
    3C-Protein A-10His-TwinStrep and BiP Secretion Pe…
    Expression
    Insect
    Promoter
    Fused Actin-HSP70
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    175443-plasmid-map-sequence-id-360135

    Enlarge

  18. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-StrepII (Human autophagy E3-like enzyme)

    Plasmid
    #169076
    Purpose
    Insect codon optimized ATG genes (excluded tags):10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-TEV-StrepII
    Depositor
    Sascha Martens
    Article
    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151
    Inserts
    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16 (ATG16L1 Human)
    Tags
    GGlinker, TEV cleavage site, StrepII and HIS10 Ta…
    Expression
    Bacterial and Insect
    Promoter
    Polyhedrin promoter
    Available Since
    May 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169076-plasmid-map-sequence-id-416841

    Enlarge

  26. RABGEF1A-c014

    Plasmid
    #175478
    Purpose
    Protein expression in insect cells. Entire CDS, M1-G491. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    RABGEF1 (RABGEF1 Human)
    Tags
    His6-Zbasic-TEV
    Expression
    Insect
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175478-plasmid-map-sequence-id-353251

    Enlarge

  27. pDest-633

    Plasmid
    #161877
    Purpose
    Standard Gateway (attR1-attR2) vector for expression of His6-GST fusions using the Bac-to-Bac insect cell system
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Tags
    His6-GST
    Expression
    Insect
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    161877-plasmid-map-sequence-id-327078

    Enlarge

  33. PPT1

    Plasmid
    #25205
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PPT1_02 (PPT1 Human)
    Tags
    His
    Expression
    Insect
    Available Since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. PSL1180polyUBdsRED

    Plasmid
    #49327
    Purpose
    Donor plasmid for homologous recombination that expresses dsRED constitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
    Depositor
    Leslie Vosshall
    Insert
    dsRED
    Expression
    Insect
    Promoter
    Aedes polyubiquitin
    Available Since
    Nov. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    49327-plasmid-map-sequence-id-73022

    Enlarge

  39. pFastBac1-His10TEV

    Plasmid
    #159427
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Tags
    TEV-protease-cleavable His10-tag
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    159427-plasmid-map-sequence-id-333995

    Enlarge

Showing: 401 - 440 of 4932 results