5,038 results
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Plasmid#46294PurposeA codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.DepositorInsertcodon optimized Cas9
UseCRISPRTags3x FlagExpressionInsectMutationSilent change from C to A at bp 834 of insertPromoterHsp70Available SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBP-split-intein-Gal4[21-881]-C-int
Plasmid#199202PurposeGateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expressionDepositorInsertsplit-intein-Gal4[21-881]-C-int
ExpressionInsectAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK33/Cas9
Plasmid#133781PurposeExpresses drosophila-optimized Cas9DepositorInsertDrosophila-optimized Cas9
ExpressionInsectAvailable SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASt-GBP-Trbo
Plasmid#179996PurposeExpresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASt version is ideal for expression in somatic tissues.DepositorInsertGBP nanobody
TagsTrboExpressionInsectAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac LIC cloning vector (4A)
Plasmid#30111DepositorTypeEmpty backboneExpressionInsectAvailable SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-wt_mScarlet-i_SGFP2
Plasmid#85071PurposeGreen/red FRET biosensor to monitor RhoA nucleotide loading state and activityDepositorInsertRhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
Tags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLIB-HIS-TEV-Cyclin-B
Plasmid#177012PurposeGenerate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEVDepositorInsertCyclin B1 (CCNB1 Human)
TagsPolyhistidine-TEVExpressionInsectMutationcodon-optimised for insect cell expressionAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBP-split-intein-Gal4[1-20]-N-int
Plasmid#199201PurposeGateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expressionDepositorInsertsplit-intein-Gal4[1-20]-N-int
ExpressionInsectAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIB-HIS-TEV-CKS1
Plasmid#177013PurposeGenerate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEVDepositorInsertCKS1 (CKS1B Human, Synthetic)
TagsPolyhistidine-TEVExpressionInsectMutationcodon-optimised for insect cell expressionAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWALIUM-intron 3x UAS attB
Plasmid#206360Purposeincludes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequencesDepositorTypeEmpty backboneExpressionInsectAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)
Plasmid#55222DepositorTypeEmpty backboneTagsSNAPf and TwinStrepIIExpressionInsectAvailable SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFL
Plasmid#110739PurposePlasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP siteDepositorTypeEmpty backboneExpressionInsectPromoterPolyhedrin and p10 promotersAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLIB-HIS-TEV-scCAK1
Plasmid#177014PurposeGenerate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEVDepositorInsertCAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
TagsPolyhistidine-TEVExpressionInsectAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pJFRC156-21XUAS-B2RT>-dSTOP-B2RT>-myr::RFP
Plasmid#32135DepositorInsertB2RT> STOP STOP B2RT>
ExpressionInsectAvailable SinceNov. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
LexAop2-myr::4xSNAPf
Plasmid#87638PurposeFor creating drosophila transgenics expressing LexAop2-myr4x::SNAPfDepositorInsertmyr::4xSNAPf
ExpressionInsectAvailable SinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBPLexA::p65Uw
Plasmid#26231DepositorInsertLexA::p65 (RELA Human)
ExpressionInsectAvailable SinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
Dll3ICD-HPC4 pBacPAK8-3
Plasmid#17334DepositorAvailable SinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBPGAL80Uw-4
Plasmid#26235DepositorInsertGAL80 (GAL80 Budding Yeast)
ExpressionInsectAvailable SinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NTOM20-LOV2
Plasmid#81009PurposeExpression of LOV2 anchored to mitochondriaDepositorInsertLOV2
TagsNTOM20ExpressionBacterial, Insect, and Mamm…PromoterCMV, p10Available SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
20XUAS IVS Jaws mCherry_tr
Plasmid#111549PurposeOptogenetics in Drosophila: UAS-induced expression of Jaws mCherryDepositorInsertJaws_mCherry_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pBPnlsLexA::GADflUw
Plasmid#26232DepositorInsertnlsLexA::GADfl
ExpressionInsectAvailable SinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGE-attBTT-loxP-DsRed (pTL780)
Plasmid#115160PurposeIntegration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.DepositorInsertattB-TT loxP 3xP3-DsRed
UseCre/LoxExpressionInsectAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVP16 AD
Plasmid#165822PurposeVP16 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domainDepositorInsertVP16 AD
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFBMBD4-C-GFP (pc1567)
Plasmid#211714PurposeExpresses fl. MBD4 GFP tagged in insect cellsDepositorInsertMBD4
TagsGFPExpressionInsectPromoterPolyhedrin PromoterAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-PA-Rac1
Plasmid#22027PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
Tags6xHis and mCherryExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-Cdc42-2G
Plasmid#68814PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)DepositorInsertCdc42 activation reporter
TagsHis and MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPINEneo-3C-GFP
Plasmid#53534PurposepTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus systemDepositorInsertGFP
TagsGFP-His8ExpressionBacterial, Insect, and Mamm…Available SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST-pHuji-GINKO2
Plasmid#177117PurposeGenetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusionDepositorInsertpHuji-GINKO2
ExpressionInsectPromoterhsp70 promoterAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFB-His-SKP1
Plasmid#228368Purposeexpress human SKP1 in insect cells, such as Trichoplusia ni Hi5DepositorAvailable SinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pF1-Hs Cdk1/cyclin B1
Plasmid#177330PurposeCo-expression of the human active Cdk1/cyclin B1 kinase complexDepositorTags2 x Strep-tag and 8 x HisExpressionInsectAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG2ab
Plasmid#80616PurposepBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-Bip-GFP:V5:KDEL
Plasmid#69917PurposeAn endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.DepositorInsertGFP
TagsDrosophila BiP signal sequence, KDEL ER targeting…ExpressionInsectPromoterpMTAvailable SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual His6 MBP N10 TEV LIC cloning vector (5C)
Plasmid#30123DepositorTypeEmpty backboneTagsHis6-MBP-N10-TEVExpressionInsectAvailable SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-PUbECFP
Plasmid#47917PurposeDonor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.DepositorInsertECFP
TagsNLSExpressionInsectPromoterAedes aegypti polyubiquitin (PUb)Available SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWal10-roe-sfGFP
Plasmid#240239PurposeGateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.DepositorTypeEmpty backboneUseGateway CloningExpressionInsectAvailable SinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAC-ie1-DsRed-ObirOrco-QF2-15xQUAS-GCaMP6s
Plasmid#200400PurposeExpresses DsRed broadly, expresses QF2 and GCaMP6s in OSNs of the clonal raider antDepositorInsertsDiscosoma red fluorescent protein
Q factor 2
GCaMP6s
ExpressionInsectPromoter15x QUAS, ObirOrco (Oocearaea biroi Orco promoter…Available SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-20XUAS-IVS-mCD8::GFP
Plasmid#26220DepositorInsertmCD8::GFP
ExpressionInsectAvailable SinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFastbac1 Nterminal GST
Plasmid#128058PurposeBaculovirus expression vector with N terminal GSTDepositorTypeEmpty backboneTagsGSTExpressionInsectAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA FLARE.sc Biosensor WT
Plasmid#12150DepositorAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-pGC-A
Plasmid#186626PurposepFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)DepositorInsertNPR1 (NPR1 Human)
Tagshemagglutinin (HA) signal peptide + His10-tag + T…ExpressionInsectMutationdeleted amino acids 1-32PromoterpolyhedringAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGS-M1-BRG1-3xFLAG-BAF57-BAF60A
Plasmid#109190PurposePlasmid for expression of C-terminally 3xFLAG-tagged BRG1, untagged BAF57 and untagged BAF60A under the control of a p10 promoter in baculovirus/insect cell systemsDepositorTags3xFLAGExpressionInsectPromoterp10Available SinceJuly 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSR646
Plasmid#65215PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-6 cap
UseAAVExpressionInsectPromoterP10 and polyhedrinAvailable SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10
Plasmid#218866PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8f
Tags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationQ315LPromoterhsp70Available SinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC102
Plasmid#118883Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C proteaseDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tubp-Gal80 in pCaSpeR4
Plasmid#17748DepositorAvailable SinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBIG1D-eIF3bgi
Plasmid#240040PurposeHuman eIF3b, eIF3g and eIF3i subunits for further cloning or expression in insect cells for purificationDepositorInserteIF3b, g, i
TagseIF3i: HIS10ExpressionInsectMutationWTPromoterPolyhedrinAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10
Plasmid#162386PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8s
Tags6xHisExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
RP125 pUAST EGFP:Orco
Plasmid#53441PurposeN-terminal fusion of EGFP to Drosophila melanogaster OrcoDepositorAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only