5,017 results
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Plasmid#134903PurposeBaculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tagDepositorTypeEmpty backboneTags3x-FlagExpressionInsectPromoterpolyhedronAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only
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pUb PspCas13b + PspCas13b guide RNA
Plasmid#176305PurposeExpresses PspCas13b and its associated guide RNA in Drosophila cellsDepositorInsertPspCas13b
TagsHA tagExpressionInsectMutationE113K, N265S- please depositor commentsPromoterUbi-p63eAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
ANO10 (TMEM16KA-c001)
Plasmid#110267PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab5
Plasmid#31733DepositorAvailable SinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_wt_Venus_wt
Plasmid#39813DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
R989-X31-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L
Plasmid#159558PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61LDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L (KRAS Human)
TagsHis6-MBP-tevExpressionInsectMutationQ61LAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1067A1
Plasmid#164847PurposeExpresses four gRNA targeting BTub gene with 3xp3-eGFP markerDepositorInsert4 gRNAs and 3xP3-eGFP
ExpressionInsectAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Ac-AChBP
Plasmid#63128PurposeExpression of Aplysia californica AChBPDepositorInsertAChBP
ExpressionInsectPromoterPolyhedrinAvailable SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-UAP56 WT
Plasmid#110125PurposeExpresses Flag-tagged human UAP56 (DDX39B)DepositorAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHoY110_pAC8_hsDCAF5_FL
Plasmid#249183PurposeExpress DCAF5(full length) in Insect cellsDepositorInsertDCAF5 full length (DCAF5 Human)
TagsStrep_BirA_short-linker-TEVExpressionInsectMutationN/AAvailable SinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-TBK1
Plasmid#221331PurposeTBK1 protein overexpression in Sf9 insect cellsDepositorAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Rab10wt
Plasmid#135557PurposeExpress mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.DepositorAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1067K
Plasmid#164848PurposeExpresses four gRNA targeting Mhc gene with 3xp3-tdTomato markerDepositorInsert4 gRNAs and 3xP3-tdTomato
ExpressionInsectAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-874PA
Plasmid#164846PurposeExpresses AAEL005635-Cas9 with OpIE2-CFP markerDepositorInsertP10-3'UTR OpIE2-CFP
ExpressionInsectAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJFRC150-20XUAS-IVS-Flp1::PEST
Plasmid#32132DepositorInsertFlippase1::PEST
TagsPESTExpressionInsectMutationAspartic Acid at Amino Acid 5Available SinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
UAS-3xHalo7::CAAX
Plasmid#87646PurposeFor creating drosophila transgenics expressing UAS-3xHalo7::CAAXDepositorInsert3xHalo7::CAAX
ExpressionInsectAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2
Plasmid#87644PurposeFor creating drosophila transgenics expressing UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2DepositorInsertmyr::SNAPf>myr::CLIPf>myr::Halo2
ExpressionInsectAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDR121
Plasmid#37148DepositorAvailable SinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTiger-OptoSOS
Plasmid#86439PurposeSingle-construct blue light optogenetic Ras activator for use in DrosophilaDepositorInsertOptoSOS
TagstagRFPExpressionInsectPromoterGal4-UASAvailable SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQF2WB
Plasmid#61312PurposeEnhancer trap version of QF2, analogous to pGAWBDepositorInsertQF2
ExpressionInsectAvailable SinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-frame_selector_0,1,2
Plasmid#131152PurposeDrosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-inDepositorInsertCRISPaint frame selector sgRNAs 0,1,2
UseCRISPRExpressionInsectPromoterdU6:3Available SinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
AeU6 3p3 GFP LgRNA
Plasmid#182208PurposeFor generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegyptiDepositorInsert3xP3-GFP
UseCRISPRExpressionInsectPromoterU6Available SinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_FLAG-BPTF
Plasmid#102242PurposeExpression of human Flag-BPTF using baculovirus from pFastBac1DepositorAvailable SinceDec. 11, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAC-dCLK
Plasmid#33153DepositorAvailable SinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRDB_053
Plasmid#216083Purposefor transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vectorDepositorHas ServiceCloning Grade DNAInsertgypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
UseDestinationExpressionInsectAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pFastBac Dual α1B/β3 Tubulin
Plasmid#170552PurposeEncodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.DepositorTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-IMD-V5-His
Plasmid#63748PurposeExpresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoterDepositorInsertimmune deficiency (imd Fly)
TagsV5-6XHisExpressionInsectMutation*see comment belowPromoterpMTAvailable SinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLIB-WDR61
Plasmid#231729PurposeProtein expression in insect cellsDepositorInsertWDR61 (SKIC8 Human)
ExpressionInsectAvailable SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSR660
Plasmid#65216PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-8 cap
UseAAVExpressionInsectPromoterP10 and polyhedrinAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pWalium-ChtVis-Tomato
Plasmid#67756PurposeExpresses a fluorescent chitin reporter under Gal4 control in Drosophila.DepositorInsertdyl - chitin binding domain - tdTomato - dyl
Tagstd-tomatoExpressionInsectAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHD-T2A-split-intein-Gal4[1-20]-N-int
Plasmid#199203PurposeTo create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-intDepositorInsertT2A-split-intein-Gal4[1-20]-N-int
ExpressionInsectAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAST-attB-yki:V5-S111A, S168A, S250A
Plasmid#85620PurposeExpresses V5 tagged triple mutant yki in Drosophila.DepositorInsertyki-V5-S111A,S168,S250A
TagsV5ExpressionInsectAvailable SinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
RP156 pUAST EGFP:Gr21a
Plasmid#59524PurposeN-terminal fusion of EGFP to Drosophila melanogaster Gr21aDepositorAvailable SinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-LIC_DDB1:1-1140_delta:396-689
Plasmid#210897PurposeInsect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.DepositorInsertDDB1:M1-H1140_delta:I396-D689 (DDB1 Human)
ExpressionInsectMutationloop deletion amino acids I396 to D689PromoterPolyhedrinAvailable SinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUAS-kkv::NG
Plasmid#138953PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectAvailable SinceNov. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_SEC31A:1-338
Plasmid#210925PurposeInsect expression of human SEC31A fragment M1 to T338. N-terminal 6X His tag with TEV cleavage.DepositorInsertSEC31A:M1-T338 (SEC31A Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCoofy27
Plasmid#44003DepositorTypeEmpty backboneTagsHis6ExpressionInsectPromoterPPHAvailable SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
1154B_gFLE_pB_VTK_ActG
Plasmid#187238PurposeAn. gambiae transgenesis plasmid. PiggyBac with transposase in backbone. Expresses two gRNAs targeting fleDepositorInsertFemaleless
UseCRISPRExpressionInsectAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-QtgRNA2.1-8R
Plasmid#170514PurposegRNA cloning vector containing a Gal80 Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6:3Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_SNF2L-opt_p48
Plasmid#102243PurposeExpression of human codon optimized human SNF2L and pRbAp48 from pFastBac dualDepositorExpressionInsectMutationcodon optimized for expressionPromoterPolyhedrin and p10Available SinceNov. 1, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAc5.1B-EGFP-MBP_V
Plasmid#147736PurposeInsect Expression vectorDepositorTypeEmpty backboneExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1
Plasmid#213025PurposeExpresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cellsDepositorInsertLINE-1 ORF2p (1-1275)
Tags3C-3xFlag (ORF2p)ExpressionInsectPromoterPolyhedrinAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-NS
Plasmid#149546PurposeExpression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6Available SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMi[3xP3::mCherry-SV40, Plodia-FibL::mBaojin-P10]
Plasmid#240410PurposeFor Minos transgenesis in insect, expresses the 3xP3::mCherry eye/glia transgenesis, and the mBaoJin in Posterior Silk Glands (under the control of the Fibroin-L promoter from Plodia interpunctella)DepositorInsertsExpressionInsectPromoter3xP3 and Fibroin-L proximal promoter (Plodia inte…Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-10H-SIII
Plasmid#175444PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneTags3C-10His-TwinStrep and BiP Secretion PeptideExpressionInsectPromoterFused Actin-HSP70Available SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
LRP1A-c004
Plasmid#205112PurposeLRP1 protein expressionDepositorAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -