5,038 results
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Plasmid#175363PurposeZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cellsDepositorInsertsgGFP
ExpressionInsectMutationF64L, S65C, and I167TPromoterFused Actin-HSP70Available SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual
Plasmid#216377PurposeBaculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)DepositorInsertLDL receptor related protein 6 (LRP6 Human)
UseBaculovirusTags9xHis and HA signal sequence-FLAG-3C cleavage sit…ExpressionInsectPromoterPolyhedrin (LRP6)/p10 (MESD)Available SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAC-DsRed-QF2-hsp70
Plasmid#104876PurposeGeneral purpose vector for cloning enhancer/promoter regions to drive QF2 expression; used for mosquitoDepositorInsertQF2
ExpressionInsectAvailable SinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HRW-CRY2olig-VHH(GFP)
Plasmid#126026Purposeexpress RFP-tagged LARIAT variant for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHRolig-VHH(GFP)
TagsRFPExpressionInsectMutationCRY2 E490GPromoterHsp70Available SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opie2-NeoR
Plasmid#131614PurposeExpresses NeoR and dsRed.DepositorInsertsNeoR
dsRed
ExpressionInsectPromoterOpie2 and hr5Ie1Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT mCherry-CaaX
Plasmid#126669PurposeInducible expression of mCherry-CaaX in S2 cellsDepositorInsertmCherry-CaaX
ExpressionInsectAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-PA-Rac1
Plasmid#22024PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
Tags6X HisExpressionBacterial, Insect, and Mamm…MutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[closed]
Plasmid#71810PurposeGenerating transgenic flies for FLP-inducible DamIDDepositorInsertmin.hsp70P-FRT-STOP#1-FRT-Dam-Myc[closed]
ExpressionInsectAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-3xFLAG-hSpCas9
Plasmid#162161PurposeExpresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)DepositorInsertshSpCas9
sgRNA
UseCRISPRTags3xFLAGExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1C-eIF3ac
Plasmid#240039PurposeHuman eIF3a and eIF3c subunits for further cloning or expression in insect cells for purificationDepositorInserteIF3a, c
TagseIF3a: TEV_3xFLAG and eIF3c: 3C_STREP IIExpressionInsectMutationWTPromoterPolyhedrinAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBIG1e-CDK9/Cyclin T1
Plasmid#231732PurposeProtein expression in insect cellsDepositorAvailable SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFL-EGFP-3xFlag-HLTF
Plasmid#193055PurposeBaculovirus expression of human HLTF for recombinant protein productionDepositorAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
6X-STAT-synthetic luciferase
Plasmid#37392DepositorInsertsix STAT consensus sites
UseLuciferaseExpressionInsectPromoterHsp70Available SinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHY002_Strep_Avi_hsDCAF15
Plasmid#174479PurposeExpresses DCAF15 in insect cellsDepositorAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUA
Plasmid#58372PurposeUAS vector to express gene of interest in DrosophilaDepositorTypeEmpty backboneExpressionInsectAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAS-PE2-attB
Plasmid#149550PurposeExpression of PE2 enzyme under control of the Gal4-regulated UAS promoter. Can be used to generate transgenic flies with white+ selection.DepositorInsertPE2
UseCRISPRExpressionInsectPromoterUASAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
13XLexAop2 IVS ChrimsonR mCherry_tr
Plasmid#111548PurposeOptogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tubp-Gal4 in pCaSpeR4
Plasmid#17747DepositorAvailable SinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSPLp10
Plasmid#170462PurposeEarlyBac insect cell expression systemDepositorInsertNew vector
ExpressionInsectPromoterdHsp70Available SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
tRECs/3xUAS-FP attB
Plasmid#220330Purposeencodes three Fluorescent Protein FLP-OUT cassettes responsive to three different recombinases (mFLP5, B3 and KD)DepositorInsertsB3RT-STOP-B3RT-TagBFP-3xHA
KDRT-STOP-KDRT-GFP-HSV
FRTm71-STOP-FRTm71-mCherry
ExpressionInsectPromoterUASAvailable SinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE2-puro
Plasmid#208032PurposeEnables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.DepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVL1392 TMEM97
Plasmid#157748PurposeBaculovirus transfer vectors to create a virus expressing human TMEM97DepositorAvailable SinceAug. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP-Rab5
Plasmid#31733DepositorAvailable SinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJFRC12-10XUAS-IVS-myr::GFP
Plasmid#26222DepositorInsertmyr::GFP
ExpressionInsectAvailable SinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A-mMD2-proA
Plasmid#187878Purposebaculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tagDepositorAvailable SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
PRExpress
Plasmid#122486PurposeA non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)DepositorInsertsLacZ
poly-PRE
ExpressionInsectAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::Vkg::mCherry
Plasmid#163929PurposeExpression of GrabFP-ECM under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 nanobody with Viking (Collagen IV) protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationG1767DAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHsp83-NLS-HA-2xMCP-2xTagRFP-T
Plasmid#71242PurposeUsed to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.DepositorInsertNLS-2xMCP-2xTagRFP-T
UseP element-mediated germline transformationTagsTagRFP-TExpressionInsectPromoterHsp83Available SinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTiger-OptoSOS
Plasmid#86439PurposeSingle-construct blue light optogenetic Ras activator for use in DrosophilaDepositorInsertOptoSOS
TagstagRFPExpressionInsectPromoterGal4-UASAvailable SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18c
Plasmid#135555PurposeExpress mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.DepositorInsertMunc18c
Tags6xHis tag and TEV siteExpressionInsectPromoterpolyhedrin promoterAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
UAS-<FRT.STOP<Bxb1
Plasmid#87643PurposeFor creating drosophila transgenics expressing UAS-<FRT.STOP<Bxb1DepositorInsertBxb1
ExpressionInsectAvailable SinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBdest-GST-PI3Kc1
Plasmid#187992PurposePlasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326DepositorTagsGSTExpressionBacterial and InsectAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-Q63L_mScarlet-i_SGFP2
Plasmid#85072PurposeGreen/red FRET biosensor for RhoA in the GTP locked constitutively active stateDepositorInsertRhoA Biosensor constitutively active (Q63L) (RHOA Human)
Tags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…MutationQ63LPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hPMS2
Plasmid#129425PurposeExpression human PMS2 protein using baculovirusDepositorAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-15-LexABD-G4MD-QFAD
Plasmid#46105DepositorInsertLexABD-G4MD-QFAD
ExpressionInsectAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pECIA14
Plasmid#47051PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tagsDepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsAlkaline Phosphatase (human, placental), Flag, HR…ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
R57C10-Flp2
Plasmid#63172PurposeR57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusionDepositorInsertyeast wild type containing a Glycine at amino acid 5 of the protein sequence
ExpressionInsectAvailable SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-URH49 WT
Plasmid#110127PurposeExpresses Flag-tagged human URH49 (DDX39A)DepositorAvailable SinceApril 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR660
Plasmid#65216PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-8 cap
UseAAVExpressionInsectPromoterP10 and polyhedrinAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>QF2
Plasmid#80275PurposeGAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.DepositorInsertQF2
ExpressionInsectAvailable SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_FLAG-BPTF
Plasmid#102242PurposeExpression of human Flag-BPTF using baculovirus from pFastBac1DepositorAvailable SinceDec. 11, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBC1FG
Plasmid#31346DepositorAvailable SinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (11C)
Plasmid#48296DepositorTypeEmpty backboneTagsHis6 and MBPExpressionInsectAvailable SinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac WT SOX
Plasmid#192055PurposeExpression vector for WT SOXDepositorInsertWT SOX (ORF37)
ExpressionInsectPromoterPolyhedrinAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-melHis10TEV
Plasmid#159428Purposeempty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sitesDepositorTypeEmpty backboneTagsmelittin signal sequence (removed in vivo by sign…ExpressionInsectPromoterpolyhedrinAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B-Aalb_743
Plasmid#176676PurposeExpression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIZ-Flag6His-Siwi
Plasmid#50560PurposeExpresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect cellsDepositorAvailable SinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abc
Plasmid#80617PurposepBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLIB-FLAG-GST(N)-RPB1-CTD(1586-1971)
Plasmid#231721PurposeProtein expression in insect cellsDepositorAvailable SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBP2MF
Plasmid#31279DepositorAvailable SinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only